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001-es BibID:	BIBFORM043761
Első szerző:	Fári Miklós (kertészmérnök)
Cím:	Agrobacterium-mediated genetic-transformation and plant-regeneration via organogenesis and somatic embryogenesis from cotyledon leaves in eggplant (solanum-melongena L CV kecskemeti-lila / Fari M., Nagy I., Csanyi ., Mityko J., Andrasfalvy A.
Dátum:	1995
ISSN:	0721-7714
Megjegyzések:	Novel and efficient protocols for plant regeneration genetic transformation longitudinally-halved cotyledons of in vitro seedlings in eggplant (Solanum melongena L.) are described. After co-cultivation with Agrobacterium vectors harboring neomycin phosphotransferase (nptll) as selectable marker, transgenic plantlets were regenerated on selective media containing 100 mg/l kanamycin. Transformants were recovered from embryogenic calli induced by 4 mg/l alpha-naphthaleneacetic acid (NAA), and from organogenic calli induced by the addition of 2 mg/l zeatin plus 0.01 mg/l NAA. Nineteen independent transgenic lines were grown to maturity. The structural integrity, expression and sexual transmission of the introduced genes for neomycin phosphotransferase and beta-glucuronidase (gus) were investigated.
Tárgyszavak:	Agrártudományok Növénytermesztési és kertészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Plant cell reports. - 15 : 1-2 (1995), p. 82-86. -
További szerzők:	Nagy István (agrár) Csányi M. Mitykó J. Andrásfalvy A.
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001-es BibID:	BIBFORM043764
Első szerző:	Mitykó J.
Cím:	Anther-culture response in different genotypes and F1 hybrids of pepper (Capsicum-Annuum-L.) / Mityko J., Andrasfalvy A., Csillery G., Fari M.
Dátum:	1995
ISSN:	0179-9541
Megjegyzések:	An in vitro anther-culture method has been improved by using young mother plants and by using frequent subcultures, thus increasing the androgenic yield in different Capsicum annuum L. genotypes. An assortment of peppers was used, composed of 15 genotypes (four breeding lines, seven cultivars and four F-1 hybrids). A new system for qualifying the androgenic response was established. For use in practical breeding, a minimum of 5% of plant regeneration was proposed as the criterion for a fair response. Accordingly, one excellent, one good and eight fair responses were identified among the genotypes investigated. As compared to the standard cultivar, 2 genotypes gave a significantly better response, i.e. 'Feherozon' (75.8%) and 'Szechuan 90716' (21.0%). In comparative investigations, F-1 hybrids, produced from crosses between poor/non-responsive and responsive genotypes, showed a fair level of response, even the case of a poor response in donor parent. The ploidy level of the resulting plants was determined by flow-cytometric analysis.
Tárgyszavak:	Agrártudományok Növénytermesztési és kertészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Plant breeding. - 114 : 1 (1995), p. 78-80. -
További szerzők:	Andrásfalvy A. Csilléry Gábor Fári Miklós (1953-) (kertészmérnök)
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