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001-es BibID:	BIBFORM043763
Első szerző:	Bakos A.
Cím:	Plant-regeneration from seedling-derived callus of dodder (Cuscuta-Trifolii bab et Giggs) / Bakos A., Fari M., Toldi O., Lados M.
Dátum:	1995
ISSN:	0168-9452
Megjegyzések:	Callus was induced and plants were regenerated for Cuscuta trifolii, the obligatory parasite of Medicago sativa. Callus was initiated on young seedlings grown in liquid culture medium consisting of Murashige and Skoog basal salts, Gamborg's vitamins (MSB5), 5 mg/l kinetin and 0.75 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D). The structure of callus tissue depended on the carbohydrates supplied, Friable, fast growing calluses were obtained in the presence of maltose and sorbitol combined (1.5-1.5%) and was maintained on corresponding agar-solidified medium for over a year. Plants were regenerated from calluses grown on solidified MSB5 medium that either lacked growth regulators or contained 2 mg/l kinetin. Embryo-like forms developed continuously from this culture for over 5 months. The detached embryo-like structures were placed in liquid MSB5 medium containing 2 mg/l kinetin and they grew as seedlings would under the same conditions. Both embryo-like structures and seedlings developed aggregate structures from which shoots grew. In this way, Cuscuta plants could be maintained in vitro without the presence of a host.
Tárgyszavak:	Agrártudományok Növénytermesztési és kertészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Plant science. - 109 : 1 (1995), p. 95-101. -
További szerzők:	Fári Miklós (1953-) (kertészmérnök) Toldi O. Lados M.
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