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001-es BibID:	BIBFORM104362
Első szerző:	Kaszás László (kertészmérnök)
Cím:	Finding valuable bioactive components from Jerusalem artichoke (Helianthus tuberosus L.) 2 leaf protein concentrate in a green biorefinery concept / László Kaszás, Tarek Alshaal, Hassan El-Ramady, Zoltán Kovács, Judit Koroknai, Nevien Elhawat, Éva Nagy, Zoltán Cziáky, Miklós Fári, Éva Domokos-Szabolcsy
Dátum:	2019
Megjegyzések:	Jerusalem artichoke is widely known for its inulin-enriched tubers. Recently the opportunity has been arisen to involve the whole plant in biorefinery concept due to its high lignocellulose biomass and tuber production. This paper focuses on the repeatedly harvestable green biomass of Jerusalem artichoke. Ultra-High Performance Liquid Chromatography-Electrospray Ionization/Mass Spectrometry (UHPLC-ESI-MS) was applied to identify the phytochemicals in Jerusalem artichoke leaf protein concentrate (JAPC) thermally extracted from green biomass of three clones, i.e., Alba, Fuseau and Kalevala. Amino acid and fatty acid profiles as well as yield of JAPC were also analyzed. The UHPLC-ESI-MS analyses showed that no toxic phytochemicals were identified in JAPC. The results revealed, also, that JAPC is not only essential-amino acids-rich but also contains substantial amounts of polyunsaturated fatty acids (66-68%) such as linolenic and linoleic acids. Linolenic acid represented 39-43% of total lipid content; moreover, the ratio between {omega}-6 and {omega}-3 essential fatty acids in JAPC was ~0.6: 1. Using UHPLC-ESI-MS, the following hydroxylated methoxyflavones were for the first time identified in JAPC, i.e., dimethoxy-tetrahydroxyflavone, dihydroxy-methoxyflavone, hymenoxin and nevadensin. These compounds are medically important since they are referred to as anti-cancer, anti-inflammatory and antioxidants. Also, liquiritigenin - estrogenic-like compound - was identified in JAPC alongside the following terpenes, i,e., loliolide and dihydroactinidiolide. However, no remarkable differences of phytochemicals, fatty acids and amino acids composition were seen among Jerusalem artichoke clones. The green biomass of tested clones ranged between 5 to 5.6 kg m-2 and JAPC yield varied from 28.6 to 31.2 g DM kg-1 green biomass with total protein content, on average, of 33.3%. According to our knowledge, this paper is the first scientific report highlighting bioactive substances in JAPC such as PUFA phytochemicals. These results clearly prove that JAPC is a valuable product which can direct towards human and animal nutrition as well as it can serve as basic material for different industrial purposes.
Tárgyszavak:	Agrártudományok Növénytermesztési és kertészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Jerusalem artichoke leaf protein concentrate polyunsaturated fatty acids phytochemicals amino acids UHPLC-ESI-MS
Megjelenés:	bioRxiv Plant Biology. - [Epub ahead of print] (2019). -
További szerzők:	Alshaal, Tarek Ali Ahmed Ibrahim (1980-) (Soil Scientist) El-Ramady, Hassan (1969-) (agrármérnök) Kovács Zoltán (1992-) (biotechnológus) Koroknai Judit Elhawat, Nevien Adel (1984-) (agrármérnök) Nagy Éva Cziáky Zoltán Fári Miklós (1953-) (kertészmérnök) Domokos-Szabolcsy Éva (1978-) (molekuláris biológus)
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001-es BibID:	BIBFORM087128
035-os BibID:	(cikkazonosító)889 (WOS)000558768700001 (Scopus)85087945888
Első szerző:	Kaszás László (kertészmérnök)
Cím:	Identification of Bioactive Phytochemicals in Leaf Protein Concentrate of Jerusalem Artichoke (Helianthus tuberosus L.) / László Kaszás, Tarek Alshaal, Hassan El-Ramady, Zoltán Kovács, Judit Koroknai, Nevien Elhawat, Éva Nagy, Zoltán Cziáky, Miklós Fári, Éva Domokos-Szabolcsy
Dátum:	2020
ISSN:	2223-7747
Tárgyszavak:	Agrártudományok Növénytermesztési és kertészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk circular economy green biorefinery polyunsaturated fatty acids phytochemicals amino acids food and feed UHPLC-ESI-ORBITRAP-MS/MS Növényélettani és produkcióbiológiai folyamatok szerepe az abiotikus és biotikus stressz hatások meghatározásában
Megjelenés:	Plants-Basel. - 9 : 7 (2020), p. 1-17. -
További szerzők:	Alshaal, Tarek Ali Ahmed Ibrahim (1980-) (Soil Scientist) El-Ramady, Hassan (1969-) (agrármérnök) Kovács Zoltán (1992-) (biotechnológus) Koroknai Judit Elhawat, Nevien Adel (1984-) (agrármérnök) Nagy Éva (1990-) (agrár) Cziáky Zoltán Fári Miklós (1953-) (kertészmérnök) Domokos-Szabolcsy Éva (1978-) (molekuláris biológus)
Pályázati támogatás:	EFOP-3.6.2-16-2017-00001 EFOP NKFIH-1150-6/2019 Egyéb János Bolyai Research Scholarship of the Hungarian Academy of Sciences Egyéb
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001-es BibID:	BIBFORM084846
035-os BibID:	(Scopus)85082927583
Első szerző:	Kaszás László (kertészmérnök)
Cím:	Refining high-quality leaf protein and valuable co-products from green biomass of Jerusalem artichoke (Helianthus tuberosus L.) for sustainable protein supply / László Kaszás, Tarek Alshaal, Zoltán Kovács, Judit Koroknai, Nevien Elhawat, Éva Nagy, Hassan El-Ramady, Miklós Fári, Éva Domokos-Szabolcsy
Dátum:	2020
ISSN:	2190-6815 2190-6823
Megjegyzések:	The present study evaluates the green biomass of Jerusalem artichoke (JA) as an alternative green protein. A leaf protein concentrate (LPC) was prepared from leafy shoots using biotechnological methods. Seven clones were compared to assess the importance of the genetic basis of JA, and alfalfa served as the control. The LPC content of JA was an average 39 g kg?1 of fresh biomass, while that of alfalfa was 32 g kg?1 . The JA can produce up to 936 kg of protein ha?1 year?1 without fertilization under rainfed conditions. The crude protein content of the LPC varied from 24.2 to 31.4 m/m%, depending on clones and harvesting time, which was comparable to that of alfalfa LPC (~ 32.3%). The amino acid profile of the LPC of JA, particularly of the essential amino acids, was similar to alfalfa and soybean. In addition, our results confirmed that the polyunsaturated fatty acid (PUFA) content varied between 64 and 68% in the LPC fraction, regardless of the clone that was used, with linoleic acid and linolenic acid being the predominant PUFAs. In addition, unlike alfalfa, the content of arachidonic acid was 0.5% in the JA LPC. The tuber yield was significantly reduced because of the repeated harvesting of the shoot parts; however, the tubers obtained were sufficient to regenerate the plantation in the subsequent year, thus ensuring the renewable ability and sustainability of the green biomass of JA.
Tárgyszavak:	Agrártudományok Növénytermesztési és kertészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Leaf protein concentrate Jerusalem artichoke Clones Amino acids Polyunsaturated fatty acids Circular economy
Megjelenés:	Biomass Conversion and Biorefinery. - 10 (2020), p. 1-16. -
További szerzők:	Alshaal, Tarek Ali Ahmed Ibrahim (1980-) (Soil Scientist) Kovács Zoltán (1992-) (biotechnológus) Koroknai Judit Elhawat, Nevien Adel (1984-) (agrármérnök) Nagy Éva (1990-) (agrár) El-Ramady, Hassan (1969-) (agrármérnök) Fári Miklós (1953-) (kertészmérnök) Domokos-Szabolcsy Éva (1978-) (molekuláris biológus)
Pályázati támogatás:	ÚNKP-19-4-DE-281 Egyéb EFOP-3.6.2-16-2017-00001 EFOP
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