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001-es BibID:BIBFORM042216
035-os BibID:PMID:16102115
Első szerző:Dale, George L.
Cím:Quantitation of microparticles released from coated-platelets / G. L. Dale, Gy. Remenyi, P. Friese
Dátum:2005
ISSN:1538-7933
Megjegyzések:Dual agonist stimulation of platelets with thrombin and convulxin results in generation of coated-platelets, a sub-population of cells known formerly as COAT-platelets (collagen and thrombin). Coated-platelets retain several procoagulant proteins on their surface and express phosphatidylserine (PS). In this report, we utilize a new methodology to demonstrate that coated-platelets also release microparticles. Platelets were prelabeled with 2.5 microm Bodipy-maleimide and then stimulated with convulxin plus thrombin. Microparticles, 0.3-0.5 microm in diameter, were observed by fluorescence confocal microscopy. Confocal microscopy was also used to demonstrate that microparticles were positive for glycoprotein IIb/IIIa, glycoprotein Ib, CD9, and PS, but negative for fibrinogen and thrombospondin. Furthermore, microparticles released from Bodipy-labeled platelets were observed by flow cytometry, and activation with convulxin plus thrombin produced 15 +/- 5 microparticles per coated-platelet. In contrast, platelets stimulated with thrombin or convulxin alone produced few microparticles. Phenylarsine oxide and diamide, both of which potentiate the mitochondrial permeability transition pore and coated-platelet production, significantly increased the number of microparticles released per coated-platelet.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
convulxin
brinogen
microparticle
platelet
thrombin
thrombospondin.
külföldön készült közlemény
Megjelenés:Journal of Thrombosis and Haemostasis. - 3 : 9 (2005), p. 2081-2088. -
További szerzők:Friese, Paul Reményi Gyula (1969-) (belgyógyász, haematológus)
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2.

001-es BibID:BIBFORM042215
035-os BibID:PMID:19658001
Első szerző:Dale, George L.
Cím:Tetraspanin CD9 is required for microparticle release from coated-platelets / George L. Dale, Gyula Remenyi, Paul Friese
Dátum:2009
ISSN:0953-7104
Megjegyzések:CD9, a member of the tetraspanin superfamily, is the third most abundant protein on the platelet surface, but its function remains unknown. In this report, we demonstrate that CD9 is required for the release of microparticles from coated-platelets. Coated-platelets are formed as a result of dual agonist activation with collagen and thrombin, and each coated-platelet releases 15?25 microparticles averaging 0.4 ?m in diameter. We report here that four separate monoclonal antibodies against CD9 inhibited microparticle release from coated-platelets by 72?102% with an IC50 of approximately 500 ng/mL for ALB6 and SN4. In addition, the anti-?IIb?3 monoclonal antibody AP2 also inhibited microparticle release although additional anti-?IIb?3 monoclonals did not. These data support participation of the tetraspanin CD9, together with the integrin ?IIb?3, in the membrane vesiculation process associated with platelet microparticle release.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
platelet
microparticle
CD9
coated-platelet
integrin
külföldön készült közlemény
Megjelenés:Platelets. - 20 : 6 (2009), p. 361-366. -
További szerzők:Friese, Paul Reményi Gyula (1969-) (belgyógyász, haematológus)
Internet cím:Szerző által megadott URL
DOI
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3.

001-es BibID:BIBFORM042217
035-os BibID:PMID:15591217
Első szerző:Reményi Gyula (belgyógyász, haematológus)
Cím:Role of mitochondrial permeability transition pore in coated-platelet formation / Gyula Remenyi, Robert Szasz, Paul Friese, George L. Dale
Dátum:2005
ISSN:1079-5642
Megjegyzések:OBJECTIVE:Coated-platelets are a subset of cells observed during costimulation of platelets with collagen and thrombin. Important characteristics of coated-platelets include retention of multiple alpha-granule proteins and expression of phosphatidylserine on the cell surface. The mitochondrial permeability transition pore (MPTP) is a key step in apoptosis and is suggested to be involved in some forms of platelet activation. The objective of this study was to examine the role of MPTP in the synthesis of coated-platelets.METHODS AND RESULTS:Flow cytometric analysis of coated-platelet production was used to examine the impact of pharmacological effectors of MPTP formation. Cyclosporin A, coenzyme Q, and bongkrekic acid all inhibit MPTP formation as well as production of coated-platelets. Phenylarsine oxide and diamide, both potentiators of MPTP formation, stimulate coated-platelet synthesis. Atractyloside, another inducer of MPTP formation, does not affect the percentage of coated-platelets synthesized; however, it does increase the level of phosphatidylserine exposed on the surface of coated-platelets.CONCLUSIONS:These findings indicate that MPTP formation is an integral event in the synthesis of coated-platelets. Although the exact function of the MPTP remains to be determined, these data support a growing body of evidence that apoptosis-associated events are vital components of the platelet activation process. Formation of coated-platelets involves a complex set of activation events initiated by dual agonist activation. The mitochondrial permeability transition pore (MPTP) is a key intermediate in apoptosis and has been suggested to impact platelet activation. This report demonstrates that MPTP formation is essential to production of coated-platelets.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
coated platelet
mitochondrial permeability transition pore
coenzyme Q
phosphatidylserine
cyclosporin A
phenylarsine oxide
diamide
külföldön készült közlemény
Megjelenés:Arteriosclerosis Thrombosis and Vascular Biology. - 25 : 2 (2005), p. 467-471. -
További szerzők:Szász Róbert (1972-) (belgyógyász, haematológus) Friese, Paul Dale, George L.
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DOI
Intézményi repozitóriumban (DEA) tárolt változat
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