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1.

001-es BibID:BIBFORM004703
035-os BibID:(scopus)0037013728 (wos)000176059200014
Első szerző:Damjanovich Sándor (biofizikus)
Cím:Does mosaicism of the plasma membrane at molecular and higher hierarchical levels in human lymphocytes carry information on the immediate history of cells? / Damjanovich, S., Matyus, L., Damjanovich, L., Bene, L., Jenei, A., Matko, J., Gaspar, R., Szollosi, J.
Dátum:2002
Megjegyzések:A theoretical analysis of experimental data is presented in this mini-review on non-random homo- and hetero-associations of cell surface receptors, which can be recruited in the plasma membrane or at the surface of the rough endoplasmic reticulum during the protein synthesis. In the latter case, the likely genetic origin of these supramolecular formations is analyzed, contrasting this concept to the mobility of the cell surface proteins. A model is offered which, on the one hand, allows the mobility in a restricted way even among microdomain-confined receptor proteins through 'swapping partners'. On the other hand, the lack of mixing molecular components of protein clusters will be analyzed, when homo-and hetero-associations are studied through cell fusion experiments. The most frequently studied cell surface patterns have included lipid raft organized HLA class I and II, ICAM-1, tetraspan molecules, IL2 and IL15 and other receptors, as well. On the contrary coated pit-associated transferrin receptors would not mix with the above lipid raft associated receptor patterns, although transferrin receptor would readily oligomerize into homo-associates. The functional consequences of these superstructures are also analyzed. On the 30th anniversary of the Singer-Nicolson fluid mosaic membrane model one has to pay tribute to the authors, because of their deep insight emphasizing also the mosaicism of the membranes in general and that of the plasma membrane, in particular.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
analysis
Biophysics
Cell Fusion
Cells
Human
Hungary
Lymphocytes
Proteins
egyetemen (Magyarországon) készült közlemény
Megjelenés:Immunology Letters. - 82 : 1-2 (2002), p. 93-99. -
További szerzők:Mátyus László (1956-) (biofizikus) Damjanovich László (1960-) (általános sebész) Bene László (1963-) (biofizikus) Jenei Attila (1966-) (biofizikus) Matkó János (1952-) (biológus) Gáspár Rezső (1944-) (biofizikus) Szöllősi János (1953-) (biofizikus)
Internet cím:DOI
elektronikus változat
Borító:

2.

001-es BibID:BIBFORM015619
Első szerző:Fábián Ákos István (aneszteziológus)
Cím:Strength in numbers : effects of Acceptor Abundance on FRET Efficiency / Ákos I. Fábián, Tünde Rente, János Szöllősi, László Mátyus, Attila Jenei
Dátum:2010
Megjegyzések:Fluorescence resonance energy transfer (FRET) is a strongly distance-dependent process between a donor and an acceptor molecule, which can be used for sensitive distance measurements and characterization of molecular interactions at the nanometer level. The original mathematical description of this process, however, is only valid for the interaction of one donor with one acceptor. This criterion is not always met, especially in biological systems, where multiple structures can interact simultaneously, often making distance estimations based on transfer efficiency values error-prone. Herein we investigate how the interaction of multiple acceptors and donors influences the transfer efficiency value in an intramolecular cellular FRET system by manipulating the fluorophore/protein ratio of the fluorophore-conjugated antibodies. We show that the labeling ratio of the acceptor has the largest influence on measured transfer efficiency and decreasing or increasing the acceptor labeling ratio can be utilized to manipulate the FRET response of the acceptor-donor pair and therefore is a tool for optimizing sensitivity of FRET measurements
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Antibodies
article
BINDING-SITE
BY-CELL BASIS
cell surface receptors
chemistry
Energy Transfer
Molekuláris Medicina
FLOW
Flow Cytometry
Fluorescence
FLUORESCENCE RESONANCE
Fluorescence Resonance Energy Transfer
FRET
HIGH-THROUGHPUT FRET
Hungary
IMAGING MICROSCOPY
LIVING CELLS
molecular interaction
PROTEIN INTERACTIONS
resonance energy transfer
RESONANCE ENERGY-TRANSFER
SPECTROSCOPIC RULER
OTKA::1
MAB::3.1
egyetemen (Magyarországon) készült közlemény
Megjelenés:Chemphyschem. - 11 : 17 (2010), p. 3713-3721. -
További szerzők:Rente Tünde Szöllősi János (1953-) (biofizikus) Mátyus László (1956-) (biofizikus) Jenei Attila (1966-) (biofizikus)
Pályázati támogatás:TÁMOP-4.2.1/B-09/1/KONV-2010-0007
TÁMOP
Membrán dinamika
K 77600
OTKA
K 68763
OTKA
TÁMOP-4.2.2-08/1-2008-0019
TÁMOP
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
DOI
Borító:

3.

001-es BibID:BIBFORM114595
035-os BibID:(scopus)85169450797 (WOS)001058799600001
Első szerző:Kormos József (fizikus)
Cím:HLA DQ protein changes the cell surface distribution pattern of HLA proteins as monitored by Förster resonance energy transfer and high-resolution electron microscopy / Kormos József, Veres Adrienn J., Imre László, Mátyus László, Benkő Szilvia, Szöllősi János, Jenei Attila
Dátum:2023
ISSN:1552-4922 1552-4930
Megjegyzések:Peptide presentation by MHC class I and MHC class II molecules plays important roles in the regulation of the immune response. One factor in these displays is the density of antigen, which must exceed a critical threshold for the effective activation of T cells. Nonrandom distribution of MHC class I and class II has already been detected at the nanometer level and at higher hierarchical levels. It is not clear how the absence and reappearance of some protein molecules can influence the nonrandom distribution. Therefore, we performed experiments on HLA II-deficient bare lymphocyte syndrome (BLS1) cells: we created a stable transfected cell line, tDQ6-BLS-1, and were able to detect the effect of the appearance of HLA-DQ6 molecules on the homo and heteroassociation of different cell surface molecules by comparing Förster resonance energy transfer (FRET) efficiency on transfected cells to that on nontransfected BLS-1 and JY human B-cell lines. Our FRET results show a decrease in homoassociation FRET between HLA I chains in HLA-DQ6-transfected tDQ6-BLS-1 cells compared with the parent BLS-1 cell line and an increase in heteroassociation FRET between HLA I and HLA II (compared with JY cells), suggesting a similar pattern of antigen presentation by the HLA-DQ6 allele. Transmission electron microscopy (TEM) revealed that both HLA class I and class II molecules formed clusters at higher hierarchical levels on the tDQ6-BLS-1 cells, and the de novo synthesized HLA DQ molecules did not intersperse with HLA class I islands. These observations could be important in understanding the fine tuning of the immune response.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
BLS-1
FRET
HLA-DQ6
immunogold labeling
MHC
TEM
Megjelenés:Cytometry Part A. - 103 : 12 (2023), p. 978-991. -
További szerzők:Veres Adrienn J. (biofizikus) Imre László (1979-) (biológus) Mátyus László (1956-) (biofizikus) Benkő Szilvia (1973-) (molekuláris biológus) Szöllősi János (1953-) (biofizikus) Jenei Attila (1966-) (biofizikus)
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

4.

001-es BibID:BIBFORM023492
Első szerző:Matkó János (biológus)
Cím:Analysis of cell surface molecular distributions and cellular signaling by flow cytometry / J. Matkó, L. Mátyus, J. Szöllősi, L. Bene, A. Jenei, P. Nagy, A. Bodnár, S. Damjanovich
Dátum:1994
ISSN:1053-0509
Megjegyzések:Flow cytometry is a fast analysis and separation method for large cell populations, based on collection and processing of optical signals gained on a cell-by-cell basis. These optical signals are scattered light and fluorescence. Owing to its unique potential ofStatistical data analysis and sensitive monitoring of (micro)heterogeneities in large cell populations, flow cytometry?in combination with microscopic imaging techniques?is a powerful tool to study molecular details of cellular signal transduction processes as well. The method also has a widespread clinical application, mostly in analysis of lymphocyte subpopulations for diagnostic (or research) purposes in diseases related to the immune system. A special application of flow cytometry is the mapping of molecular interactions (proximity relationships between membrane proteins) at the cell surface, on a cell-by-cell basis. We developed two approaches to study such questions; both are based ondistance-dependent quenching of excited state fluorophores (donors) by fluorescent or dark (nitroxide radical) acceptors via Förstertype dipole-dipole resonance energy transfer (FRET) and long-range electron transfer (LRET) mechanisms, respectively. A critical evaluation of these methods using donor- or acceptor-conjugated monoclonal antibodies (or their Fab fragments) to select the appropriate cell surface receptor or antigen will be presented in comparison with other approaches for similar purposes. The applicability of FRET and LRET for two-dimensional antigen mapping as well as for detection of conformational changes in extracellular domains of membrane-bound proteins is discussed and illustrated by examples of several lymphoma cell lines. Another special application area of flow cytometry is the analysis of different aspects of cellular signal transduction, e.g., changes of intracellular ion (Ca2+, H+, Na+) concentrations, regulation of ion channel activities, or more complex physiological responses of cell to external stimuli via correlated fluorescence and scatter signal analysis, on a cell-by-cell basis. This way different signaling events such as changes in membrane permeability, membrane potential, cell size and shape, ion distribution, cell density, chromatin structure, etc., can be easily and quickly monitored over large cell populations with the advantage of revealing microheterogeneities in the cellular responses. Flow cytometry also offers the possibility to follow the kinetics of slow (minute- and hour-scale) biological processes in cell populations. These applications are illustrated by the example of complex flow cytometric analysis of signaling in extracellular ATP-triggered apoptosis (programmed cell death) of murine thymic lymphocytes.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
fluorescence
flow cytometry
energy transfer
electron transfer
protein-protein interaction
signal transduction
egyetemen (Magyarországon) készült közlemény
Megjelenés:Journal Of Fluorescence 4 : 4 (1994), p. 303-314. -
További szerzők:Mátyus László (1956-) (biofizikus) Szöllősi János (1953-) (biofizikus) Bene László (1963-) (biofizikus) Jenei Attila (1966-) (biofizikus) Nagy Péter (1971-) (biofizikus) Dóczy-Bodnár Andrea (1970-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus)
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DOI
Intézményi repozitóriumban (DEA) tárolt változa
Borító:

5.

001-es BibID:BIBFORM005089
Első szerző:Mátyus László (biofizikus)
Cím:Steady-state fluorescence quenching applications for studying protein structure and dynamics / Matyus, L., Szollosi, J., Jenei, A.
Dátum:2006
ISSN:011-1344 (Print)
Megjegyzések:Fluorescence quenching methods are useful to obtain information about the conformational and/or dynamic changes of proteins in complex macromolecular systems. In this review steady-state methods are described and the data interpretation is thoroughly discussed. As a special case of fluorescence quenching mechanism, fluorescence resonance energy transfer (FRET) phenomenon is also presented. Application of a FRET based method to characterize the temperature dependence of the flexibility of protein matrix is clearly demonstrated.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Acrylamide
Algorithms
Biophysics
Cesium
chemistry
Chlorides
Energy Transfer
Fluorescence
Fluorescence Resonance Energy Transfer
Hungary
Macromolecular Systems
metabolism
methods
Protein Conformation
Proteins
Research
Support
Temperature
Tryptophan
egyetemen (Magyarországon) készült közlemény
Megjelenés:Journal of Photochemistry and Photobiology. B, Biology. - 83 : 3 (2006), p. 223-236. -
További szerzők:Szöllősi János (1953-) (biofizikus) Jenei Attila (1966-) (biofizikus)
Internet cím:elektronikus változat
DOI
Borító:

6.

001-es BibID:BIBFORM044572
Első szerző:Nagy Péter (biofizikus)
Cím:Activation-dependent clustering of the erbB2 receptor tyrosine kinase detected by scanning near-field optical microscopy / Péter Nagy, Attila Jenei, Achim K. Kirsch, János Szöllősi, Sándor Damjanovich, Thomas M. Jovin
Dátum:1999
Megjegyzések:ErbB2 (HER2, Neu), a member of the epidermal growth factor (EGF) receptor tyrosine kinase family, is often overexpressed in breast cancer and other malignancies. ErbB2 homodimerizes but also presents as a common auxiliary subunit of the EGF and heregulin receptors (erbB1 or EGFR; and erbB3-4, respectively), with which it heteroassociates. ErbB2 is generally regarded as an orphan (ligand-less) receptor with a very potent kinase domain activated either via its associated partners or constitutively as a consequence of discrete mutations. It follows that the extent and regulation of its cell surface interactions are of central importance. We have studied the large-scale association pattern of erbB2 in quiescent and activated cells labeled with fluorescent anti-erbB2 monoclonal antibodies using scanning near-field optical microscopy (SNOM). ErbB2 was found to be concentrated in irregular membrane patches with a mean diameter of approx. 0.5 microm in nonactivated SKBR3 and MDA453 human breast tumor cells. The average number of erbB2 proteins in a single cluster on nonactivated SKBR3 cells was about 10(3). Activation of SKBR3 cells with EGF, heregulin as well as a partially agonistic anti-erbB2 monoclonal antibody led to an increase in the mean cluster diameter to 0.6-0.9 microm, irrespective of the ligand. The EGF-induced increase in the erbB2 cluster size was inhibited by the EGFR-specific tyrosine kinase inhibitor PD153035. The average size of erbB2 clusters on the erbB2-transfected line of CHO cells (CB2) was similar to that of activated SKBR3 cells, a finding correlated with the increased base-line tyrosine phosphorylation of erbB2 in cells expressing only erbB2. We conclude that an increase in cluster size may constitute a general phenomenon in the activation of erbB2.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Animal
antagonists and inhibitors
chemistry
Cho Cells
Enzyme Activation
Enzyme Inhibitors
Hamsters
Human
metabolism
methods
Microscopy
Microscopy, Atomic Force
Microscopy, Confocal
pharmacology
Quinazolines
Receptor Protein-Tyrosine Kinases
Receptor, Epidermal Growth Factor
Receptor, erbB-2
Support, Non-U.S.Gov't
Tumor Cells, Cultured
egyetemen (Magyarországon) készült közlemény
Megjelenés:Journal of Cell Science 112 : Pt 11 (1999), p. 1733-1741. -
További szerzők:Jenei Attila (1966-) (biofizikus) Kirsch, Achim K. Szöllősi János (1953-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Jovin, Thomas M.
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
Szerző által megadott URL
Borító:

7.

001-es BibID:BIBFORM004687
Első szerző:Nagy Péter (biofizikus)
Cím:Cell fusion experiments reveal distinctly different association characteristics of cell-surface receptors / Péter Nagy, László Mátyus, Attila Jenei, György Panyi, Sándor Varga, János Matkó, János Szöllősi, Rezső Gáspár, Thomas M. Jovin, Sándor Damjanovich
Dátum:2001
Megjegyzések:The existence of small- and large-scale membrane protein clusters, containing dimers, oligomers and hundreds of proteins, respectively, has become widely accepted. However, it is largely unknown whether the internal structure of these formations is dynamic or static. Cell fusion was used to perturb the distribution of existing membrane protein clusters, and to investigate their mobility and associations. Scanning near-field optical microscopy, confocal and electron microscopy were applied to detect the exchange of proteins between large-scale protein clusters, whereas photobleaching fluorescence energy transfer was used to image the redistribution of existing small-scale membrane protein clusters. Large-scale clusters of major histocompatibility complex (MHC)-I exchanged proteins with each other and with MHC-II clusters. Similarly to MHC-I, large-scale MHC-II clusters were also dynamic. Exchange of components between small-scale protein clusters was not universal: intermixing did not take place in the case of MHC-II homoclusters; however, it was observed for homoclusters of MHC-I and for heteroclusters of MHC-I and MHC-II. These processes required a fluid state of the plasma membrane, and did not depend on endocytosis-mediated recycling of proteins. The redistribution of large-scale MHC-I clusters precedes the intermixing of small-scale clusters of MHC-I indicating a hierarchy in protein association. Investigation of a set of other proteins (alpha subunit of the interleukin 2 receptor, CD48 and transferrin receptor) suggested that a large-scale protein cluster usually exchanges components with the same type of clusters. These results offer new insight into processes requiring time-dependent changes in membrane protein interactions.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Biophysics
Cell Fusion
Cell Line
Cell Membrane
chemistry
Dyes
Energy Transfer
Fluorescence
Fluorescent Dyes
Gold Colloid
Histocompatibility Antigens
Histocompatibility Antigens Class I
Histocompatibility Antigens Class II
Human
Hungary
Interleukin-2
Major Histocompatibility Complex
Membrane Microdomains
metabolism
methods
Microscopy
Microscopy,Fluorescence
physiology
Proteins
Receptor Aggregation
Receptors,Cell Surface
Receptors,Interleukin-2
Support,Non-U.S.Gov't
Megjelenés:Journal of Cell Science 114 : Pt 22 (2001), p. 4063-4071. -
További szerzők:Mátyus László (1956-) (biofizikus) Jenei Attila (1966-) (biofizikus) Panyi György (1966-) (biofizikus) Varga Sándor (1943-) (biofizikus) Matkó János (1952-) (biológus) Szöllősi János (1953-) (biofizikus) Gáspár Rezső (1944-) (biofizikus) Jovin, Thomas M. Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:elektronikus változat
Szerző által megadott URL
Borító:

8.

001-es BibID:BIBFORM004650
035-os BibID:(scopus)0033367737
Első szerző:Nagy Péter (biofizikus)
Cím:Complexity of signal transduction mediated by ErbB2 : clues to the potential of receptor-targeted cancer therapy / Nagy, P., Jenei, A., Damjanovich, S., Jovin, T. M., Szollosi, J.
Dátum:1999
Megjegyzések:The erbB2 oncogene belongs to the type I trans-membrane tyrosine kinase family of receptors. Its medical importance stems from its widespread over-expression in breast cancer. This review will focus on the signal transduction through this protein, and explains how the overexpression of erbB2 may result in poor prognosis of breast cancer, and finally it will summerize our current understanding about the therapeutic potential of receptor-targeted therapy in breast cancer. ErbB2 does not have any known ligand which is able to bind to it with high affinity. However the kinase activity of erbB2 can be activated without any ligand, if it is overexpressed, and by heteroassociation with other members of the erbB family (erbB1 or epidermal growth factor receptor, erbB3 and erbB4). This interaction substantially increases the efficiency and diversity of signal transduction through these receptor complexes. In addition, erbB2 forms large scale receptor clusters containing hundreds of proteins. These receptor islands may take part in recruiting cytosolic factors which relay the signal towards the nucleus or the cytoplasm. Overexpression of erbB2 was linked to higher transforming activity, increased metastatic potential, angiogenesis and drug resistence of breast tumor in laboratory experiments. As a corollary of these properties, erbB2 amplification is generally thought to be associated with a poor prognosis in breast cancer patients. These early findings lead to the development of antibodies that down-regulate erbB2. Such a therapeutic approach has already been found effective in experimental tumor models and in clinical trials as well. Further understanding of the importance of erbB2 and growth factor receptors in the transformation of normal cells to malignant ones may once give us a chance to cure erbB2 over-expressing breast cancer
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Animal
Antibodies, Monoclonal
Antineoplastic Agents
Breast Neoplasms
Cytoplasm
drug therapy
Female
Gene Therapy
Genes, erbB-2
genetics
Human
Hungary
Neoplasms
physiology
Receptor, erbB-2
Signal Transduction
Support, Non-U.S.Gov't
therapeutic use
therapy
egyetemen (Magyarországon) készült közlemény
Megjelenés:Pathology and Oncology Research. - 5 : 4 (1999), p. 255-271. -
További szerzők:Jenei Attila (1966-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Jovin, Thomas M. Szöllősi János (1953-) (biofizikus)
Internet cím:DOI
elektronikus változat
Borító:

9.

001-es BibID:BIBFORM062699
035-os BibID:(scopus)84925853989 (wos)000354040400007
Első szerző:Shrestha, Dilip (biológus)
Cím:Understanding FRET as a Research Tool for Cellular Studies / Dilip Shrestha, Attila Jenei, Péter Nagy, György Vereb, János Szöllősi
Dátum:2015
ISSN:1661-6596 1422-0067
Megjegyzések:Communication of molecular species through dynamic association and/or dissociation at various cellular sites governs biological functions. Understanding these physiological processes require delineation of molecular events occurring at the level of individual complexes in a living cell. Among the few non-invasive approaches with nanometer resolution are methods based on Förster Resonance Energy Transfer (FRET). FRET is effective at a distance of 1?10 nm which is equivalent to the size of macromolecules, thus providing an unprecedented level of detail on molecular interactions. The emergence of fluorescent proteins and SNAP- and CLIP- tag proteins provided FRET with the capability to monitor changes in a molecular complex in real-time making it possible to establish the functional significance of the studied molecules in a native environment. Now, FRET is widely used in biological sciences, including the field of proteomics, signal transduction, diagnostics and drug development to address questions almost unimaginable with biochemical methods and conventional microscopies. However, the underlying physics of FRET often scares biologists. Therefore, in this review, our goal is to introduce FRET to non-physicists in a lucid manner. We will also discuss our contributions to various FRET methodologies based on microscopy and flow cytometry, while describing its application for determining the molecular heterogeneity of the plasma membrane in various cell types.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
FRET
Megjelenés:International Journal Of Molecular Sciences. - 16 : 4 (2015), p. 6718-6756. -
További szerzők:Jenei Attila (1966-) (biofizikus) Nagy Péter (1971-) (biofizikus) Vereb György (1965-) (biofizikus, orvos) Szöllősi János (1953-) (biofizikus)
Pályázati támogatás:MTA-DE
MTA
Sejtbiológiai és Jelátvitel Kutatócsoport
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

10.

001-es BibID:BIBFORM048687
035-os BibID:PMID:22027563 WOS:000300859500001
Első szerző:Shrestha, Dilip (biológus)
Cím:Bare lymphocyte syndrome : an opportunity to discover our immune system / Shrestha Dilip, Szöllősi János, Jenei Attila
Dátum:2012
ISSN:0165-2478
Megjegyzések:Bare lymphocyte syndrome (BLS) is a rare immunodeficiency disorder manifested by the partial or complete disappearance of major histocompatibility complex (MHC) proteins from the surface of the cells. Based on this specific feature, it is categorized into three different types depending on which type of MHC protein is affected. These proteins are mainly involved in generating the effective immune responses by differentiating 'self' from 'non-self' antigens through a process referred to as antigen presentation. Investigations on BLS have immensely contributed to our understanding of the transcriptional regulation of these molecules and have led to the discovery of several important proteins of the antigen presentation pathway. Reviews on this subject consistently project type II BLS, MHC II deficiency as BLS syndrome, although literatures' document cases of other types of BLS too. Therefore, in this article, we have assembled information on the BLS syndrome to produce a systematic narration while emphasizing the importance of BLS system in studying various aspects of immune biology.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Bare lymphocyte syndrome
MHC
HLA
Transporter associated with antigen
processing
Class II trans-activator
Regulatory factor X complex
Molekuláris Medicina
Megjelenés:Immunology Letters. - 141 : 2 (2012), p. 147-157. -
További szerzők:Szöllősi János (1953-) (biofizikus) Jenei Attila (1966-) (biofizikus)
Pályázati támogatás:TÁMOP-4.2.1/B-09/1/KONV-2010-0007
TÁMOP
Membrán dinamika
K68763
Egyéb
TÁMOP-4.2.2-08/1-2008-0019
TÁMOP
TÁMOP-4.2.1/B-09/1/KONV-2010-0007
TÁMOP
Receptor tirozin kinázok mint terápiás célpontok: működésük szabályozásának, és a közöttük fellépő molekuláris kölcsönhatások vizsgálata
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DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

11.

001-es BibID:BIBFORM048515
Első szerző:Shrestha, Dilip (biológus)
Cím:CD1d favors MHC neighborhood, GM1 ganglioside proximity and low detergent sensitive membrane regions on the surface of B lymphocytes / Dilip Shrestha, Mark A. Exley, György Vereb, János Szöllősi, Attila Jenei
Dátum:2014
ISSN:0304-4165
Megjegyzések:BACKGROUND: Cluster of differentiation 1 (CD1) represents a family of proteins which is involved in lipid-based antigen presentation. Primarily, antigen presenting cells, like B cells, express CD1 proteins. Here, we examined the cell-surface distribution of CD1d, a subtype of CD1 receptors, on B lymphocytes. METHODS: Fluorescence labeling methods, including fluorescence resonance energy transfer (FRET), were employed to investigate plasma membrane features of CD1d receptors. RESULTS: High FRET efficiency was observed between CD1d and MHC I heavy chain (MHC I-HC), beta2-microglobulin (beta2m) and MHC II proteins in the plasma membrane. In addition, overexpression of CD1d reduced the expression of MHC II and increased the expression of MHC I-HC and beta2m proteins on the cell-surface. Surprisingly, beta2m dependent CD1d isoform constituted only ~15% of the total membrane CD1d proteins. Treatment of B cells with methyl-beta-cyclodextrin (MbetaCD) / simvastatin caused protein rearrangement; however, FRET demonstrated only minimal effect of these chemicals on the association between CD1d and GM1 ganglioside on cell-surface. Likewise, a modest effect was only observed in a co-culture assay between MbetaCD/simvastatin treated C1R-CD1d cells and invariant natural killer T cells on measuring secreted cytokines (IFNgamma and IL4). Furthermore, CD1d rich regions were highly sensitive to low concentration of Triton X-100. Physical proximity between CD1d, MHC and GM1 molecules was also detected in the plasma membrane. CONCLUSIONS: An intricate relationship between CD1d, MHC, and lipid species were found on the membrane of human B cells. General significance Organization of CD1d on the plasma membrane might be critical for its biological functions.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
CD1d
MHC
FRET
Rafts
Simvastatin
Megjelenés:Biochimica et Biophysica Acta (BBA). General Subjects. - 1840 : 1 (2014), p. 667-680. -
További szerzők:Exley, Mark A. (1961-) (immunológus) Vereb György (1965-) (biofizikus, orvos) Szöllősi János (1953-) (biofizikus) Jenei Attila (1966-) (biofizikus)
Internet cím:Szerző által megadott URL
DOI
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Borító:

12.

001-es BibID:BIBFORM043016
035-os BibID:PMID:22797718
Első szerző:Shrestha, Dilip (biológus)
Cím:Comparative study of the three different fluorophore antibody conjugation strategies / Dilip Shrestha, Adrienn Bagosi, János Szöllősi, Attila Jenei
Dátum:2012
Megjegyzések:The progression in bioconjugational chemistry has significantly contributed to the evolution and success of protein biology. Mainly, antibody chemistry has been a subject of intensive study owing to the expansion of research areas warranted by using various derivatives of conjugated antibodies. Three reactive moieties (amine, sulfhydryl and carbohydrate) in the antibodies are chiefly favored for the conjugational purpose. This feature is known for decades, nevertheless, amine based conjugation is still the most preferred strategy despite the appreciation the other two methods receive in conserving the antigen binding affinity (ABA). No single report has been published, according to our knowledge, where these three conjugation strategies were applied to the same fluorophore antibody systems. In this study, we evaluated conjugation yield, time demand and cost efficiency of these conjugation procedures. Our results showed that amine based conjugations was by far the best technique due to its simplicity, rapidity, ease of operation, higher conjugate yield, cheaper cost and potential for larger fluorophore/protein labeling ratio without having much effect in ABA. Furthermore, sulfhydryl labeling clearly excelled in terms of reduced non-specific binding and mild effect in ABA but was usually complicated by an asymmetric antibody reduction due to mercaptoethylamine while carbohydrate oxidation based strategy performed the worst during our experiment
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Antibodies
Antibody conjugation
article
binding affinity
Biophysics
cell biology
chemistry
Comparative Study
Hungary
methods
Research
Research Support
Support
time
egyetemen (Magyarországon) készült közlemény
Megjelenés:Analytical and Bioanalytical Chemistry - 404 : 5 (2012), p. 1449-1463. -
További szerzők:Bagosi Adrienn Szöllősi János (1953-) (biofizikus) Jenei Attila (1966-) (biofizikus)
Pályázati támogatás:TÁMOP-4.2.2-08/1-2008-0019
TÁMOP
TÁMOP-4.2.1/B-09/1/KONV-2010-007
TÁMOP
TÁMOP-4.2.2/B-10/ 1-2010-0024
TÁMOP
Internet cím:DOI
Intézményi repozitóriumban (DEA) tárolt változat
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