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001-es BibID:	BIBFORM004648
Első szerző:	Jenei Attila (biofizikus)
Cím:	Picosecond multiphoton scanning near-field optical microscopy / Jenei, A., Kirsch, A. K., Subramaniam, V., Arndt-Jovin, D. J., Jovin, T. M.
Dátum:	1999
Megjegyzések:	We have implemented simultaneous picosecond pulsed two- and three-photon excitation of near-UV and visible absorbing fluorophores in a scanning near-field optical microscope (SNOM). The 1064-nm emission from a pulsed Nd:YVO4 laser was used to excite the visible mitochondrial specific dye MitoTracker Orange CM-H2TMRos or a Cy3-labeled antibody by two-photon excitation, and the UV absorbing DNA dyes DAPI and the bisbenzimidazole BBI-342 by three-photon excitation, in a shared aperture SNOM using uncoated fiber tips. Both organelles in human breast adenocarcinoma cells (MCF 7) and specific protein bands on polytene chromosomes of Drosophila melanogaster doubly labeled with a UV and visible dye were readily imaged without photodamage to the specimens. The fluorescence intensities showed the expected nonlinear dependence on the excitation power over the range of 5-40 mW. An analysis of the dependence of fluorescence intensity on the tip-sample displacement normal to the sample surface revealed a higher-order function for the two-photon excitation compared to the one-photon mode. In addition, the sample photobleaching patterns corresponding to one- and two-photon modes revealed a greater lateral confinement of the excitation in the two-photon case. Thus, as in optical microscopy, two-photon excitation in SNOM is confined to a smaller volume
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban analysis Animal Antibodies Cells chemistry Chromosomes Dna Drosophila melanogaster Dyes Fluorescence Fluorescent Dyes genetics Human Lasers metabolism methods Microscopy Microscopy, Fluorescence Mitochondria Photons Support, Non-U.S.Gov't Tumor Cells,Cultured ultrastructure Ultraviolet Rays külföldön készült közlemény
Megjelenés:	Biophysical Journal. - 76 : 2 (1999), p. 1092-1100. -
További szerzők:	Kirsch, Achim K. Subramaniam, Vinod Arndt-Jovin, Donna J. Jovin, Thomas M.
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001-es BibID:	BIBFORM043709
035-os BibID:	PM:10999315
Első szerző:	Kirsch, Achim K.
Cím:	Fluorescence resonance energy transfer detected by scanning near-field optical microscopy / Kirsch, A. K., Subramaniam, V., Jenei, A., Jovin, T. M.
Dátum:	1999
ISSN:	0022-2720
Megjegyzések:	Fluorescence resonance energy transfer (FRET) between excited fluorescent donor and acceptor molecules occurs via the Forster mechanism over a range of 1-10 nm. Because of the strong (sixth power) distance dependence of the signal, FRET has been used to assess the proximity of molecules in biological systems. We used a scanning near-field optical microscope (SNOM) operated in the shared-aperture mode using uncoated glass fibre tips to detect FRET between dye molecules embedded in polyvinyl alcohol films and bound to cell surfaces. FRET was detected by selective photobleaching of donor and acceptor fluorophores. We also present preliminary results on pixel-by-pixel energy transfer efficiency measurements using SNOM
Tárgyszavak:	Természettudományok Fizikai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Journal Of Microscopy-Oxford. - 194 : Pt 2/3 (1999), p. 448-454. -
További szerzők:	Subramaniam, Vinod Jenei Attila (1966-) (biofizikus) Jovin, Thomas M.
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001-es BibID:	BIBFORM044572
Első szerző:	Nagy Péter (biofizikus)
Cím:	Activation-dependent clustering of the erbB2 receptor tyrosine kinase detected by scanning near-field optical microscopy / Péter Nagy, Attila Jenei, Achim K. Kirsch, János Szöllősi, Sándor Damjanovich, Thomas M. Jovin
Dátum:	1999
Megjegyzések:	ErbB2 (HER2, Neu), a member of the epidermal growth factor (EGF) receptor tyrosine kinase family, is often overexpressed in breast cancer and other malignancies. ErbB2 homodimerizes but also presents as a common auxiliary subunit of the EGF and heregulin receptors (erbB1 or EGFR; and erbB3-4, respectively), with which it heteroassociates. ErbB2 is generally regarded as an orphan (ligand-less) receptor with a very potent kinase domain activated either via its associated partners or constitutively as a consequence of discrete mutations. It follows that the extent and regulation of its cell surface interactions are of central importance. We have studied the large-scale association pattern of erbB2 in quiescent and activated cells labeled with fluorescent anti-erbB2 monoclonal antibodies using scanning near-field optical microscopy (SNOM). ErbB2 was found to be concentrated in irregular membrane patches with a mean diameter of approx. 0.5 microm in nonactivated SKBR3 and MDA453 human breast tumor cells. The average number of erbB2 proteins in a single cluster on nonactivated SKBR3 cells was about 10(3). Activation of SKBR3 cells with EGF, heregulin as well as a partially agonistic anti-erbB2 monoclonal antibody led to an increase in the mean cluster diameter to 0.6-0.9 microm, irrespective of the ligand. The EGF-induced increase in the erbB2 cluster size was inhibited by the EGFR-specific tyrosine kinase inhibitor PD153035. The average size of erbB2 clusters on the erbB2-transfected line of CHO cells (CB2) was similar to that of activated SKBR3 cells, a finding correlated with the increased base-line tyrosine phosphorylation of erbB2 in cells expressing only erbB2. We conclude that an increase in cluster size may constitute a general phenomenon in the activation of erbB2.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Animal antagonists and inhibitors chemistry Cho Cells Enzyme Activation Enzyme Inhibitors Hamsters Human metabolism methods Microscopy Microscopy, Atomic Force Microscopy, Confocal pharmacology Quinazolines Receptor Protein-Tyrosine Kinases Receptor, Epidermal Growth Factor Receptor, erbB-2 Support, Non-U.S.Gov't Tumor Cells, Cultured egyetemen (Magyarországon) készült közlemény
Megjelenés:	Journal of Cell Science 112 : Pt 11 (1999), p. 1733-1741. -
További szerzők:	Jenei Attila (1966-) (biofizikus) Kirsch, Achim K. Szöllősi János (1953-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Jovin, Thomas M.
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat Szerző által megadott URL
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001-es BibID:	BIBFORM034563
Első szerző:	Subramaniam, Vinod
Cím:	Scanning Near-Field Optical Imaging and Spectroscopy In Cell Biology / Subramaniam, V., Kirsch, A. K., Jenei, A., Jovin, T. M.
Dátum:	2000
ISSN:	978-0-471-31575-9
Tárgyszavak:	Orvostudományok Elméleti orvostudományok könyvfejezet analysis külföldön készült közlemény cell biology
Megjelenés:	Emerging Tools for Single-Cell Analysis : Advances in Optical Measurement Technologies / eds. Durack, G.; Robinson, J. P. - p. 271-290. -
További szerzők:	Kirsch, Achim K. Jenei Attila (1966-) (biofizikus) Jovin, Thomas M.
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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