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1.

001-es BibID:BIBFORM004925
Első szerző:Damjanovich Sándor (biofizikus)
Cím:Structural hierarchy in the clustering of HLA class I molecules in the plasma membrane of human lymphoblastoid cells / Damjanovich, S., Vereb, G., Schaper, A., Jenei, A., Matko, J., Starink, J. P., Fox, G. Q., Arndt-Jovin, D. J., Jovin, T. M.
Dátum:1995
Megjegyzések:Major histocompatibility complex (MHC) class I antigens in the plasma membranes of human T (HUT-102B2) and B (JY) lymphoma cells were probed by immunochemical reagents using fluorescence, transmission electron, and scanning force microscopies. Fluorescent labels were attached to monoclonal antibodies W6/32 or KE-2 directed against the heavy chain of HLA class I (A, B, C) and L368 or HB28 against the beta 2-microglobulin light chain. The topological distribution in the nanometer range was studied by photobleaching fluorescence resonance energy transfer (pbFRET) on single cells. A nonrandom codistribution pattern of MHC class I molecules was observed over distances of 2-10 nm. A second, nonrandom, and larger-scale topological organization of the MHC class I antigens was detected by indirect immunogold labeling and imaging by transmission electron microscopy (TEM) and scanning force microscopy (SFM). Although some differences in antigen distribution between the B- and T-cell lines were detected by pbFRET, both cell lines exhibited similar clustering patterns by TEM and SFM. Such defined molecular distributions on the surfaces of cells of the immune system may reflect an underlying specialization of membrane lipid domains and fulfill important functional roles in cell-cell contacts and signal transduction.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Adult
analysis
beta 2-Microglobulin
Cell Line
Cell Membrane
chemistry
Energy Transfer
Fluorescence
Gold Colloid
Histocompatibility Antigens Class I
Human
Immunohistochemistry
immunology
Light
Lymphocytes
Major Histocompatibility Complex
methods
Microscopy
Microscopy,Electron
Signal Transduction
Support,Non-U.S.Gov't
Tumor Cells,Cultured
ultrastructure
egyetemen (Magyarországon) készült közlemény
Megjelenés:Proceedings of the National Academy of Sciences of the United States of America. - 92 : 4 (1995), p. 1122-1126. -
További szerzők:Vereb György (1965-) (biofizikus, orvos) Schaper, Achim Jenei Attila (1966-) (biofizikus) Matkó János (1952-) (biológus) Starink, J. Pascual Fox, Geoffrey Q. Arndt-Jovin, Donna J. Jovin, Thomas M.
Internet cím:elektronikus változat
elektronikus változat
Borító:

2.

001-es BibID:BIBFORM046086
Első szerző:Dóczy-Bodnár Andrea (biofizikus)
Cím:Class I HLA oligomerization at the surface of B cells is controlled by exogenous [béta]2-microglobulin : implications in activation of cytotoxic T lymphocytes / Bodnar A., Bacso Z., Jenei A., Jovin T. M., Edidin M., Damjanovich S., Matko J.
Dátum:2003
ISSN:1460-2377
Megjegyzések:Submicroscopic molecular clusters (oligomers) of class I HLA have been detected by physical techniques [e.g. fluorescence resonance energy transfer (FRET) and single particle tracking of molecular diffusion] at the surface of various activated and transformed human cells, including B lymphocytes. Here, the sensitivity of this homotypic association to exogenous beta(2)-microglobulin (beta(2)m) and the role of free heavy chains (FHC) in class I HLA oligomerization were investigated on a B lymphoblastoid cell line, JY. Scanning near-field optical microscopy and FRET data both demonstrated that FHC and class I HLA heterodimers are co-clustered at the cell surface. Culturing the cells with excess beta(2)m resulted in a reduced co-clustering and decreased molecular homotypic association, as assessed by FRET. The decreased HLA clustering on JY target cells (antigen-presenting cells) was accompanied with their reduced susceptibility to specific lysis by allospecific CD8(+) cytotoxic T lymphocytes (CTL). JY B cells with reduced HLA clustering also provoked significantly weaker T cell activation signals, such as lower expression of CD69 activation marker and lower magnitude of TCR down-regulation, than did the untreated B cells. These results together suggest that the actual level of beta(2)m available at the cell surface can control CTL activation and the subsequent cytotoxic effector function through regulation of the homotypic HLA-I association. This might be especially important in some inflammatory and autoimmune diseases where elevated serum beta(2)m levels are reported.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:International Immunology. - 15 : 3 (2003), p. 331-339. -
További szerzők:Bacsó Zsolt (1963-) (biofizikus) Jenei Attila (1966-) (biofizikus) Jovin, Thomas M. Edidin, Michael Damjanovich Sándor (1936-2017) (biofizikus) Matkó János (1952-) (biológus)
Pályázati támogatás:T034393
OTKA
T030411
OTKA
F034487
OTKA
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

3.

001-es BibID:BIBFORM004648
Első szerző:Jenei Attila (biofizikus)
Cím:Picosecond multiphoton scanning near-field optical microscopy / Jenei, A., Kirsch, A. K., Subramaniam, V., Arndt-Jovin, D. J., Jovin, T. M.
Dátum:1999
Megjegyzések:We have implemented simultaneous picosecond pulsed two- and three-photon excitation of near-UV and visible absorbing fluorophores in a scanning near-field optical microscope (SNOM). The 1064-nm emission from a pulsed Nd:YVO4 laser was used to excite the visible mitochondrial specific dye MitoTracker Orange CM-H2TMRos or a Cy3-labeled antibody by two-photon excitation, and the UV absorbing DNA dyes DAPI and the bisbenzimidazole BBI-342 by three-photon excitation, in a shared aperture SNOM using uncoated fiber tips. Both organelles in human breast adenocarcinoma cells (MCF 7) and specific protein bands on polytene chromosomes of Drosophila melanogaster doubly labeled with a UV and visible dye were readily imaged without photodamage to the specimens. The fluorescence intensities showed the expected nonlinear dependence on the excitation power over the range of 5-40 mW. An analysis of the dependence of fluorescence intensity on the tip-sample displacement normal to the sample surface revealed a higher-order function for the two-photon excitation compared to the one-photon mode. In addition, the sample photobleaching patterns corresponding to one- and two-photon modes revealed a greater lateral confinement of the excitation in the two-photon case. Thus, as in optical microscopy, two-photon excitation in SNOM is confined to a smaller volume
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
analysis
Animal
Antibodies
Cells
chemistry
Chromosomes
Dna
Drosophila melanogaster
Dyes
Fluorescence
Fluorescent Dyes
genetics
Human
Lasers
metabolism
methods
Microscopy
Microscopy, Fluorescence
Mitochondria
Photons
Support, Non-U.S.Gov't
Tumor Cells,Cultured
ultrastructure
Ultraviolet Rays
külföldön készült közlemény
Megjelenés:Biophysical Journal. - 76 : 2 (1999), p. 1092-1100. -
További szerzők:Kirsch, Achim K. Subramaniam, Vinod Arndt-Jovin, Donna J. Jovin, Thomas M.
Internet cím:elektronikus változat
DOI
Borító:

4.

001-es BibID:BIBFORM043709
035-os BibID:PM:10999315
Első szerző:Kirsch, Achim K.
Cím:Fluorescence resonance energy transfer detected by scanning near-field optical microscopy / Kirsch, A. K., Subramaniam, V., Jenei, A., Jovin, T. M.
Dátum:1999
ISSN:0022-2720
Megjegyzések:Fluorescence resonance energy transfer (FRET) between excited fluorescent donor and acceptor molecules occurs via the Forster mechanism over a range of 1-10 nm. Because of the strong (sixth power) distance dependence of the signal, FRET has been used to assess the proximity of molecules in biological systems. We used a scanning near-field optical microscope (SNOM) operated in the shared-aperture mode using uncoated glass fibre tips to detect FRET between dye molecules embedded in polyvinyl alcohol films and bound to cell surfaces. FRET was detected by selective photobleaching of donor and acceptor fluorophores. We also present preliminary results on pixel-by-pixel energy transfer efficiency measurements using SNOM
Tárgyszavak:Természettudományok Fizikai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Journal Of Microscopy-Oxford. - 194 : Pt 2/3 (1999), p. 448-454. -
További szerzők:Subramaniam, Vinod Jenei Attila (1966-) (biofizikus) Jovin, Thomas M.
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
DOI
Borító:

5.

001-es BibID:BIBFORM044572
Első szerző:Nagy Péter (biofizikus)
Cím:Activation-dependent clustering of the erbB2 receptor tyrosine kinase detected by scanning near-field optical microscopy / Péter Nagy, Attila Jenei, Achim K. Kirsch, János Szöllősi, Sándor Damjanovich, Thomas M. Jovin
Dátum:1999
Megjegyzések:ErbB2 (HER2, Neu), a member of the epidermal growth factor (EGF) receptor tyrosine kinase family, is often overexpressed in breast cancer and other malignancies. ErbB2 homodimerizes but also presents as a common auxiliary subunit of the EGF and heregulin receptors (erbB1 or EGFR; and erbB3-4, respectively), with which it heteroassociates. ErbB2 is generally regarded as an orphan (ligand-less) receptor with a very potent kinase domain activated either via its associated partners or constitutively as a consequence of discrete mutations. It follows that the extent and regulation of its cell surface interactions are of central importance. We have studied the large-scale association pattern of erbB2 in quiescent and activated cells labeled with fluorescent anti-erbB2 monoclonal antibodies using scanning near-field optical microscopy (SNOM). ErbB2 was found to be concentrated in irregular membrane patches with a mean diameter of approx. 0.5 microm in nonactivated SKBR3 and MDA453 human breast tumor cells. The average number of erbB2 proteins in a single cluster on nonactivated SKBR3 cells was about 10(3). Activation of SKBR3 cells with EGF, heregulin as well as a partially agonistic anti-erbB2 monoclonal antibody led to an increase in the mean cluster diameter to 0.6-0.9 microm, irrespective of the ligand. The EGF-induced increase in the erbB2 cluster size was inhibited by the EGFR-specific tyrosine kinase inhibitor PD153035. The average size of erbB2 clusters on the erbB2-transfected line of CHO cells (CB2) was similar to that of activated SKBR3 cells, a finding correlated with the increased base-line tyrosine phosphorylation of erbB2 in cells expressing only erbB2. We conclude that an increase in cluster size may constitute a general phenomenon in the activation of erbB2.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Animal
antagonists and inhibitors
chemistry
Cho Cells
Enzyme Activation
Enzyme Inhibitors
Hamsters
Human
metabolism
methods
Microscopy
Microscopy, Atomic Force
Microscopy, Confocal
pharmacology
Quinazolines
Receptor Protein-Tyrosine Kinases
Receptor, Epidermal Growth Factor
Receptor, erbB-2
Support, Non-U.S.Gov't
Tumor Cells, Cultured
egyetemen (Magyarországon) készült közlemény
Megjelenés:Journal of Cell Science 112 : Pt 11 (1999), p. 1733-1741. -
További szerzők:Jenei Attila (1966-) (biofizikus) Kirsch, Achim K. Szöllősi János (1953-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Jovin, Thomas M.
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
Szerző által megadott URL
Borító:

6.

001-es BibID:BIBFORM004687
Első szerző:Nagy Péter (biofizikus)
Cím:Cell fusion experiments reveal distinctly different association characteristics of cell-surface receptors / Péter Nagy, László Mátyus, Attila Jenei, György Panyi, Sándor Varga, János Matkó, János Szöllősi, Rezső Gáspár, Thomas M. Jovin, Sándor Damjanovich
Dátum:2001
Megjegyzések:The existence of small- and large-scale membrane protein clusters, containing dimers, oligomers and hundreds of proteins, respectively, has become widely accepted. However, it is largely unknown whether the internal structure of these formations is dynamic or static. Cell fusion was used to perturb the distribution of existing membrane protein clusters, and to investigate their mobility and associations. Scanning near-field optical microscopy, confocal and electron microscopy were applied to detect the exchange of proteins between large-scale protein clusters, whereas photobleaching fluorescence energy transfer was used to image the redistribution of existing small-scale membrane protein clusters. Large-scale clusters of major histocompatibility complex (MHC)-I exchanged proteins with each other and with MHC-II clusters. Similarly to MHC-I, large-scale MHC-II clusters were also dynamic. Exchange of components between small-scale protein clusters was not universal: intermixing did not take place in the case of MHC-II homoclusters; however, it was observed for homoclusters of MHC-I and for heteroclusters of MHC-I and MHC-II. These processes required a fluid state of the plasma membrane, and did not depend on endocytosis-mediated recycling of proteins. The redistribution of large-scale MHC-I clusters precedes the intermixing of small-scale clusters of MHC-I indicating a hierarchy in protein association. Investigation of a set of other proteins (alpha subunit of the interleukin 2 receptor, CD48 and transferrin receptor) suggested that a large-scale protein cluster usually exchanges components with the same type of clusters. These results offer new insight into processes requiring time-dependent changes in membrane protein interactions.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Biophysics
Cell Fusion
Cell Line
Cell Membrane
chemistry
Dyes
Energy Transfer
Fluorescence
Fluorescent Dyes
Gold Colloid
Histocompatibility Antigens
Histocompatibility Antigens Class I
Histocompatibility Antigens Class II
Human
Hungary
Interleukin-2
Major Histocompatibility Complex
Membrane Microdomains
metabolism
methods
Microscopy
Microscopy,Fluorescence
physiology
Proteins
Receptor Aggregation
Receptors,Cell Surface
Receptors,Interleukin-2
Support,Non-U.S.Gov't
Megjelenés:Journal of Cell Science 114 : Pt 22 (2001), p. 4063-4071. -
További szerzők:Mátyus László (1956-) (biofizikus) Jenei Attila (1966-) (biofizikus) Panyi György (1966-) (biofizikus) Varga Sándor (1943-) (biofizikus) Matkó János (1952-) (biológus) Szöllősi János (1953-) (biofizikus) Gáspár Rezső (1944-) (biofizikus) Jovin, Thomas M. Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:elektronikus változat
Szerző által megadott URL
Borító:

7.

001-es BibID:BIBFORM004650
035-os BibID:(scopus)0033367737
Első szerző:Nagy Péter (biofizikus)
Cím:Complexity of signal transduction mediated by ErbB2 : clues to the potential of receptor-targeted cancer therapy / Nagy, P., Jenei, A., Damjanovich, S., Jovin, T. M., Szollosi, J.
Dátum:1999
Megjegyzések:The erbB2 oncogene belongs to the type I trans-membrane tyrosine kinase family of receptors. Its medical importance stems from its widespread over-expression in breast cancer. This review will focus on the signal transduction through this protein, and explains how the overexpression of erbB2 may result in poor prognosis of breast cancer, and finally it will summerize our current understanding about the therapeutic potential of receptor-targeted therapy in breast cancer. ErbB2 does not have any known ligand which is able to bind to it with high affinity. However the kinase activity of erbB2 can be activated without any ligand, if it is overexpressed, and by heteroassociation with other members of the erbB family (erbB1 or epidermal growth factor receptor, erbB3 and erbB4). This interaction substantially increases the efficiency and diversity of signal transduction through these receptor complexes. In addition, erbB2 forms large scale receptor clusters containing hundreds of proteins. These receptor islands may take part in recruiting cytosolic factors which relay the signal towards the nucleus or the cytoplasm. Overexpression of erbB2 was linked to higher transforming activity, increased metastatic potential, angiogenesis and drug resistence of breast tumor in laboratory experiments. As a corollary of these properties, erbB2 amplification is generally thought to be associated with a poor prognosis in breast cancer patients. These early findings lead to the development of antibodies that down-regulate erbB2. Such a therapeutic approach has already been found effective in experimental tumor models and in clinical trials as well. Further understanding of the importance of erbB2 and growth factor receptors in the transformation of normal cells to malignant ones may once give us a chance to cure erbB2 over-expressing breast cancer
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Animal
Antibodies, Monoclonal
Antineoplastic Agents
Breast Neoplasms
Cytoplasm
drug therapy
Female
Gene Therapy
Genes, erbB-2
genetics
Human
Hungary
Neoplasms
physiology
Receptor, erbB-2
Signal Transduction
Support, Non-U.S.Gov't
therapeutic use
therapy
egyetemen (Magyarországon) készült közlemény
Megjelenés:Pathology and Oncology Research. - 5 : 4 (1999), p. 255-271. -
További szerzők:Jenei Attila (1966-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Jovin, Thomas M. Szöllősi János (1953-) (biofizikus)
Internet cím:DOI
elektronikus változat
Borító:

8.

001-es BibID:BIBFORM034563
Első szerző:Subramaniam, Vinod
Cím:Scanning Near-Field Optical Imaging and Spectroscopy In Cell Biology / Subramaniam, V., Kirsch, A. K., Jenei, A., Jovin, T. M.
Dátum:2000
ISSN:978-0-471-31575-9
Tárgyszavak:Orvostudományok Elméleti orvostudományok könyvfejezet
analysis
külföldön készült közlemény
cell biology
Megjelenés:Emerging Tools for Single-Cell Analysis : Advances in Optical Measurement Technologies / eds. Durack, G.; Robinson, J. P. - p. 271-290. -
További szerzők:Kirsch, Achim K. Jenei Attila (1966-) (biofizikus) Jovin, Thomas M.
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
Borító:
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