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001-es BibID:	BIBFORM109031
035-os BibID:	(cikkazonosító)e83976 (WoS)000932846100001 (Scopus)85147892491
Első szerző:	Gyöngy Zsuzsanna (molekuláris biológus)
Cím:	Nucleotide binding is the critical regulator of ABCG2 conformational transitions / Gyöngy Zsuzsanna, Mocsár Gábor, Hegedűs Éva, Stockner Thomas, Ritter Zsuzsanna, Homolya László, Schamberger Anita, Orbán Tamás I., Remenyik Judit, Szakacs Gergely, Goda Katalin
Dátum:	2023
ISSN:	2050-084X
Megjegyzések:	ABCG2 is an exporter-type ABC protein that can expel numerous chemically unrelated xeno- and endobiotics from cells. When expressed in tumor cells or tumor stem cells, ABCG2 confers multidrug resistance, contributing to the failure of chemotherapy. Molecular details orchestrating substrate translocation and ATP hydrolysis remain elusive. Here, we present methods to concomitantly investigate substrate and nucleotide binding by ABCG2 in cells. Using the conformation-sensitive antibody 5D3, we show that the switch from the inward-facing (IF) to the outward-facing (OF) conformation of ABCG2 is induced by nucleotide binding. IF-OF transition is facilitated by substrates, and hindered by the inhibitor Ko143. Direct measurements of 5D3 and substrate binding to ABCG2 indicate that the high-to-low affinity switch of the drug binding site coincides with the transition from the IF to the OF conformation. Low substrate binding persists in the post-hydrolysis state, supporting that dissociation of the ATP hydrolysis products is required to reset the high substrate affinity IF conformation of ABCG2.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	eLife. - 12 (2023), p. 1-24. -
További szerzők:	Mocsár Gábor (1981-) (biofizikus) Hegedűs Éva (1978-) (biofizikus) Stockner, Thomas Ritter Zsuzsanna Homolya László Schamberger Anita Orbán Tamás I. Gálné Remenyik Judit (1965-) (kémia tanár, okleveles vegyész) Szakács Gergely Goda Katalin (1969-) (biofizikus)
Pályázati támogatás:	K124815 OTKA GINOP-2.2.1-15-2017-00079 GINOP HunProtEx 2018?1.2.1-NKP- 2018?00005 Egyéb RRF-2.3.1-21-2022-00015 Egyéb
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001-es BibID:	BIBFORM004740
035-os BibID:	(WOS)000184609400011 (scopus)12444317206
Első szerző:	Szilágyi Ildikó (orvos)
Cím:	Non-random features of loop-size chromatin fragmentation / Szilagyi, I., Varga, T., Szekvolgyi, L., Hegedus, E., Goda, K., Kaczur, V., Bacso, Z., Nakayama, Y., Posafi, J., Pongor, S., Szabo, G.
Dátum:	2003
ISSN:	730-2312 (Print)
Megjegyzések:	Upon isolation of DNA from normal eukaryotic cells by standard methods involving extensive proteolytic treatment, a rather homogeneous population of loop-size, double-stranded DNA fragments is regularly obtained. These DNA molecules can be efficiently end-labeled by the DNA polymerase I Klenow fragment, as well as by a 3'- to -5'-exonuclease-free Klenow enzyme, but not by terminal transferase (TdT) unless the ends have been filled up by Klenow, suggesting that dominantly 5' protruding termini are generated upon fragmentation. The filled-up termini were used for cloning the distal parts of the approximately 50 kb fragments. BLAST analysis of the sequence of several clones allowed us to determine the sequence of the non-cloned side of the breakpoints. Comparison of 25, 600 bp-long breakpoint sequences demonstrated prevalence of repetitive elements. Consensus motives characteristic of the breakpoint sequences have been identified. Several sequences exhibit peculiar computed conformational characteristics, with sharp transition or center of symmetry located exactly at the breakpoint. Our data collectively suggest that chromatin fragmentation involves nucleolytic cleavages at fragile/hypersensitive sites delimiting loop-size fragments in a non-random manner. Interestingly, the sequence characteristics of the breakpoints are reminiscent of certain breakpoint cluster regions frequently subject to gene rearrangements.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk analysis Animals Base Sequence Biophysics Cells chemistry Chromatin Dna DNA Fragmentation DNA Nucleotidylexotransferase DNA Polymerase I Electrophoresis,Gel,Two-Dimensional Eukaryotic Cells HL-60 Cells Humans Hungary isolation and purification Jurkat Cells methods Mice Nih 3T3 Cells Prevalence Research Sequence Analysis,DNA Support egyetemen (Magyarországon) készült közlemény
Megjelenés:	Journal of Cellular Biochemistry. - 89 : 6 (2003), p. 1193-1205. -
További szerzők:	Varga Tamás (1971-) (biológus) Székvölgyi Lóránt (1977-) (biofizikus, biokémikus, sejtbiológus) Hegedűs Éva (1978-) (biofizikus) Goda Katalin (1969-) (biofizikus) Kaczur Viktória Bacsó Zsolt (1963-) (biofizikus) Nakayama, Yuji Pósafi János Pongor Sándor Szabó Gábor (1953-) (biofizikus)
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