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001-es BibID:BIBFORM043657
Első szerző:Váradi Csaba (molekuláris biológus)
Cím:Analysis of Haptoglobin N-glycome Alterations in Inflammatory and Malignant Lung Diseases by Capillary Electrophoresis / Cs. Varadi, S. Mittermayr, A. Szekrenyes, J. Kadas, L. Takacs, I. Kurucz, A. Guttman
Dátum:2013
ISSN:0173-0835
Megjegyzések:A capillary electrophoresis based method was introduced to compare the N-glycosylation profile ofhaptoglobin in normal and pathologic conditions. To assess the biomarker potential of glycosylationchanges in various lung diseases, haptoglobin was isolated from plasma samples of healthy, pneumonia,chronic obstructive pulmonary disease (COPD) and lung cancer patients by means of two haptoglobinspecific monoclonal antibodies. Haptoglobin N-glycans were then enzymatically released, fluorescentlylabeled and profiled by capillary electrophoresis. Disease associated changes of core and antennaryfucosylation were identified by targeted exoglycosidase digestions and their levels were compared inthe different patient groups. Terms such as of core- and arm-fucosylation degree, as well as branchingdegreewere introduced for easier characterization of the changes and statistical analysis was used toexamine which structures are responsible for the observed differences. Increased level of ?1-6fucosylated tri-antennary glycans was found in all disease groups compared to the control. Elevatedamounts of core- and arm fucosylation on tetra-antennary glycans were detected in the lung cancergroup compared to the COPD group. A larger scale study is necessary to confirm and validate thesepreliminary findings in the glycosylation changes of haptoglobin, so could then be used as biomarkers inthe diagnosis of malignant and inflammatory lung diseases.
Tárgyszavak:Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
biomarker
capillary electrophoresis
Megjelenés:Electrophoresis. - 34 : 16 (2013), p. 2287-2294. -
További szerzők:Mittermayr, Stefan (1983-) (bioinformatikus) Szekrényes Ákos (1983-) (vegyészmérnök) Kádas János (1976-) (molekuláris biológus, biokémikus, kertészmérnök) Takács László (1955-) Kurucz István Guttman András (1954-) (vegyészmérnök)
Pályázati támogatás:K-81839
OTKA
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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