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001-es BibID:	BIBFORM085922
035-os BibID:	(Cikkazonosító)43156 (WOS)000394534200001 (Scopus)85013667550
Első szerző:	Qiu, Zhijun
Cím:	Generation of Gross Chromosomal Rearrangements by a Single Engineered DNA Double Strand Break / Zhijun Qiu, Zhenhua Zhang, Anna Roschke, Tamas Varga, Peter D. Aplan
Dátum:	2017
ISSN:	2045-2322
Megjegyzések:	Gross chromosomal rearrangements (GCRs), including translocations, inversions amplifications, and deletions, can be causal events leading to malignant transformation. GCRs are thought to be triggered by DNA double strand breaks (DSBs), which in turn can be spontaneous or induced by external agents (eg. cytotoxic chemotherapy, ionizing radiation). It has been shown that induction of DNA DSBs at two defined loci can produce stable balanced chromosomal translocations, however, a single engineered DNA DSB could not. Herein, we report that although a single engineered DNA DSB in H2AX "knockdown" cells did not generate GCRs, repair of a single engineered DNA DSB in fibroblasts that had ablated H2ax did produce clonal, stable GCRs, including balanced translocations and megabase-pair inversions. Upon correction of the H2ax deficiency, cells no longer generated GCRs following a single engineered DNA DSB. These findings demonstrate that clonal, stable GCRs can be produced by a single engineered DNA DSB in H2ax knockout cells, and that the production of these GCRs is ameliorated by H2ax expression.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Scientific Reports. - 7 : 1 (2017), p. 1-10. -
További szerzők:	Zhang, Zhenhua Roschke, Anna Varga Tamás (1971-) (biológus) Aplan, Peter D.
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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