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001-es BibID:	BIBFORM046502
Első szerző:	Pálvölgyi Attila
Cím:	Interplay between nitric oxide and VIP in CCK-8-induced phasic contractile activity in the rabbit sphincter of Oddi / Attila Pálvölgyi, Réka Sári, József Németh, Annamária Szabolcs, István Nagy, Péter Hegyi, János Lonovics, Zoltán Szilvássy
Dátum:	2005
ISSN:	1007-9327
Megjegyzések:	AIM: The sphincter of Oddi (SO) plays an important role indelivery of bile into the duodenum. To establish whethervasoactive intestinal polypeptide (VIP) and nitric oxide (NO)were involved in phasic contractile activity of the rabbitSO stimulated by cholecystokinin-octapeptide (CCK-8).METHODS: Isolated SO muscle rings were cleaned of fatand mounted horizontally on two small L-shaped hooksone of which was connected to a force transducer for themeasurement of isometric tension. The experiments werecarried out in a thermostatically controlled (37?0.2 )organ bath (5 mL) containing Krebs solution. The organfluid was gassed with 95% O2 and 50 mL/L CO2 to keepthe pH at 7.40?0.05. Contractile responses to CCK-8(1 ?mol/L) were evaluated in the presence and absenceof NG-nitro-L-arginine (LNNA), an inhibitor of NO synthase(100 ?mol/L), and (p-chloro-D-Phe6-Leu17)-VIP (VIPa,30 ?mol/L), a VIP receptor antagonist.RESULTS: CCK-8 stimulated the phasic activity of the SO.NO synthase inhibition increased the frequency and amplitudeof contractions with a slight increase in developed tension.Pre-incubation with VIPa also attenuated this CCK-8 effect.The combined application of LNNA and VIPa abolishedthe phasic activity of the muscle rings with a marked increasein tension in response to CCK-8.CONCLUSION: VIP and NO together contribute to anincrease in phasic activity of SO.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Sphincter of Oddi CCK VIP NO LNNA
Megjelenés:	World Journal of Gastroenterology. - 11 : 21 (2005), p. 3264-3266. -
További szerzők:	Sári Réka (farmakológus) Németh József (Pécs) Szabolcs Annamária Nagy István Hegyi Péter Jenő (belgyógyász) Lonovics János (Szeged) Szilvássy Zoltán (1957-) (belgyógyász, farmakológus, klinikai farmakológus)
Pályázati támogatás:	3.2.2.-2004-07-0001/3.0 GVOP
Internet cím:	Szerző által megadott URL Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM020575
Első szerző:	Sári Réka (farmakológus)
Cím:	Cross tolerance between nitroglycerin and neural relaxation of the rabbit sphincter of Oddi / Réka Sári, Zoltán Szilvássy, Ildikó Jakab, István Nagy, János Lonovics
Dátum:	1998
ISSN:	1043-6618
Megjegyzések:	Szegedi Tudományegyetem - Belgyógyászati Klinika We studied whether non-adrenergic, non-cholinergic (NANC) relaxation of the rabbit sphincter of Oddi was influenced by tolerance to nitroglycerin (NG)in vitro. Sphincter of Oddi (SO) muscle rings precontracted with EC50concentrations of cholecystokinin octapeptide (CCK8) were exposed to cumulative increases in NG concentrations and tested for relaxation by measurement of isometric tension. A separate group of six rings was subjected to a preceding exposure to 275 mnitroglycerin over 60 min to inducein vitrotolerance to nitroglycerin. The rings (both tolerant and non-tolerant) were subjected to electrical field stimulation (FS: 50 V, 0.1 ms, 20 Hz, 3 and 10 stimuli). The rings were then preincubated with NANC solution: phentolamine, oxprenolol and atropine (all 1 m) for 20 min and FS was applied again. FS was repeated after additional incubation withNG-nitro-l-arginine methyl ester (l-NAME), an inhibitor of NO synthase (30 m) and after a successive incubation with 3 mml-arginine (20 min). Maximum contractions produced by CCK8 in `tolerant' and 'non-tolerant' sphincters were 29.9±5.8 and 28.3±5.2 mN, respectively. The sensitivity to CCK8 also was not different between the two groups with EC50(-log M) values of 8.5±0.2 and 8.3±0.1, respectively. FS evoked twitchlike contraction followed by relaxation in the ampullary SO in both `tolerant' and `non-tolerant' preparations. Incubation in NANC solution resulted in monophasic relaxations in response to FS in non-tolerant sphincters but not in tolerant ones.l-NAME (30 m) reversed NANC relaxation in non-tolerant muscle rings whereas it failed to modify NANC contractions in the tolerant preparations.l-arginine (3 mm) reversed the inhibitory effect ofl-NAME on NANC relaxation in the `non-tolerant' rings and it was without effect on FS-induced contractions in the `tolerant' SO. As measured by radioimmunoassay, tolerance to NG was without any significant effect on tissue content of both cyclic adenosine 3:5 monophosphate (cAMP) and cyclic guanosine 3:5 monophosphate (cGMP). FS significantly increased tissue cAMP and cGMP content in 'non-tolerant' preparations. FS failed to increase the level of either cyclic nucleotide in `tolerant' tissue. We conclude that NANC relaxation of the ampullary part of the rabbit SO is significantly impaired in the state of tolerance to NG`in vitro'.
Tárgyszavak:	Orvostudományok Gyógyszerészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban cross tolerance nitroglycerin neural relaxation
Megjelenés:	Pharmacological Research. - 37 : 6 (1998), p. 505-512. -
További szerzők:	Jakab Ildikó (farmakológus) Nagy István Lonovics János (Szeged) Szilvássy Zoltán (1957-) (belgyógyász, farmakológus, klinikai farmakológus)
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM020568
Első szerző:	Szilvássy Zoltán (belgyógyász, farmakológus, klinikai farmakológus)
Cím:	Improvement of nitrergic relaxation by farnesol of the sphincter of Oddi from hypercholesterolaemic rabbits / Zoltan Szilvassy, Reka Sari, Jozsef Nemeth, Istvan Nagy, Sandor Csati, Janos Lonovics
Dátum:	1998
Megjegyzések:	AbstractField stimulation relaxed the rabbit sphincter of Oddi muscle rings after incubation with atropine (1 microM) and guanethidine (4 microM) with a threefold increase in tissue cyclic cGMP content, a response previously shown to be essentially nitrergic. Preparations from hypercholesterolaemic rabbits (1.5% dietary cholesterol load over 8 weeks increasing serum total cholesterol from pre-diet 1.4+/-0.3 to 22.6+/-3.8 mmol/l) exhibited contractions with no change in cyclic GMP content under the same conditions. The nitrergic relaxation was recaptured with a twofold increase in tissue cyclic GMP content in preparations from hypercholesterolaemic rabbits undergone a treatment with 30 microM/kg farnesol i.v. twice a day over the last 3 days of the dietary period. We conclude that farnesol treatment restores nitrergic relaxation of the sphincter of Oddi in hypercholesterolaemia.
Tárgyszavak:	Orvostudományok Gyógyszerészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban nitrergic relaxation farnesol hypercholesterolaemic
Megjelenés:	European Journal of Pharmacology. - 353 : 1 (1998), p. 75-78. -
További szerzők:	Sári Réka (farmakológus) Németh József (Pécs) Nagy István Csáti Sándor Lonovics János (Szeged)
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