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001-es BibID:BIBFORM004326
Első szerző:Gyöngyösi Mariann
Cím:Serial Noninvasive In Vivo Positron Emission Tomographic Tracking of Percutaneously Intramyocardially Injected Autologous Porcine Mesenchymal Stem Cells Modified for Transgene Reporter Gene Expression / Gyöngyösi M., Blanco J., Márián T., Trón L., Hemetsberger R., Rodriguez J., Font G., Pavo I.J., Kertész I., Balkay L., Pavo N., Posa A., Emri M., Galuska L., Kraitchman D. L., Wojta J., Huber K., Glogar D.
Dátum:2008
Megjegyzések:Background: Porcine bone marrow-derived mesenchymal stem cells (MSCs) were stably transfected with a lentiviral vector for transgene expression of the trifusion protein renilla luciferase, red fluorescent protein and herpes simplex truncated thymidine kinase (LV-RL-RFP-tTK; positron emission tomography [PET] reporter gene) for in vivo noninvasive tracking of the intramyocardially delivered MSC fate. Methods and Results: A closed-chest, reperfused myocardial infarction was created in farm pigs. Sixteen days after myocardial infarction, LV-RL-RFP-tTK-MSCs were injected intramyocardially using electromechanical mapping guidance in the infarct border zone (n=7). PET-computed tomographic metabolic and perfusion imaging was performed after an intravenous injection of 10 mCi [18F]-FHBG and 13N-ammonia PET at 30?2 hours and 7 days after LV-RL-RFP-tTK-MSC treatment. Fusion imaging of the [18F]-FHBG PET-computed tomography with MRI was used to determine the myocardial location of the injected LV-RL-RFP-tTK-MSCs. Seven days after injections, [18F]-FHBG PET showed a decreased cardiac uptake with a mild increased pericardial and pleura uptake in the treated animals, which was confirmed by the measurement of luciferase activity. At 10 days, infarct size by MRI in the LV-RL-RFP-tTK-MSC-treated animals was smaller than controls (n=7) (23.3?1.5% versus 30.2?3.5%, P<0.005). The presence of the LV-RL-RFP-tTK-MSCs (5.8?1.1% of the injected cells) in the myocardium 10 days after intramyocardial delivery was confirmed histologically.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
cells
imaging
infarction
mapping
revascularization
Megjelenés:Circulation: Cardiovascular Imaging. - 1 : 2 (2008), p. 94-103. -
További szerzők:Blanco, Jeronimo Márián Teréz (1950-) (radiobiológus) Trón Lajos (1941-) (biofizikus) Hemetsberger, Rayyan Rodriguez, Julio Font Gusztáv Pávó Imre J. Kertész István (1966-) (vegyész) Balkay László (1963-) (biofizikus) Pavo, Noemi Pósa Anikó Emri Miklós (1962-) (fizikus) Galuska László (1946-) (belgyógyász, izotópdiagnoszta) Kraitchman, Dara L. Wojta, Johann Huber, Kurt Glogar, Dietmar
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