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001-es BibID:	BIBFORM005965
Első szerző:	Balázs Margit (sejtbiológus, molekuláris genetikus)
Cím:	Accessibility of cell surface thiols in human lymphocytes is altered by ionophores or OKT-3 antibody / Margit Balázs, János Matkó, János Szöllösi, László Mátyus, Mack J. Fulwyler, Sándor Damjanovich
Dátum:	1986
Megjegyzések:	The accessibility of cell surface sulfhydryl groups in human peripheral lymphocytes was investigated with 5,5'-dithiobis-(2-nitrobenzoic acid) in the presence and absence of ionophore antibiotics and the monoclonal antibody, OKT-3. Only a few accessible protein thiols have been found on the cells as demonstrated by labeling with a fluorescent non-penetrating thiol-marker, monobromotrimethyl-ammoniobimane and the subsequent gel electrophoretic analysis of the protein pattern. Difference spectrophotometric measurement of thiol-DTNB reaction revealed that ionophores altering the transmembrane potential induced an enhanced cell surface thiol-exposure on the minute time scale. The effect showed a dependence on the external concentration of the cations. The binding of monoclonal antibody, OKT-3, directed against T3 complexes, resulted in a similar, concentration-dependent increase of thiol-accessibility. These data are interpreted as early membrane-effects of ionophores and the specific antibody including changes in the conformational equilibrium or vertical displacements of certain membrane proteins. These events are likely to be coupled to the changes in the transmembrane potential of the lymphocytes.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Ammonium Compounds analysis Antibiotics Antibodies,Monoclonal blood Cell Membrane Dithionitrobenzoic Acid drug effects Electrophoresis,Polyacrylamide Gel Human Ionophores Lymphocytes Membrane Proteins metabolism pharmacology Spectrometry,Fluorescence Sulfhydryl Compounds Support,Non-U.S.Gov't Support,U.S.Gov't,P.H.S.
Megjelenés:	Biochemical and Biophysical Research Communications. - 140 : 3 (1986), p. 999-1006. -
További szerzők:	Matkó János (1952-) (biológus) Szöllősi János (1953-) (biofizikus) Mátyus László (1956-) (biofizikus) Fulwyler, Mack J. Damjanovich Sándor (1936-2017) (biofizikus)
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001-es BibID:	BIBFORM006044
Első szerző:	Matkó János (biológus)
Cím:	Biphasic effect of extracellular ATP on the membrane potential of mouse thymocytes / Matko J., Nagy P., Panyi G., Vereb G. Jr., Bene L., Matyus L., Damjanovich S.
Dátum:	1993
Megjegyzések:	Extracellular ATP induced changes in the membrane potential of thymocytes from BALB/c mice were analyzed. At concentrations below 0.1 mM, ATP hyperpolarizes the cell membrane on the time scale of development of the Ca(2+)-signal. After a longer time hyperpolarization turns to depolarization. ATP concentrations higher than 0.5 mM caused rapid depolarization without previous hyperpolarization. Verapamil, quinine or the absence of extracellular Ca2+ blocked the hyperpolarization by ATP. In Na(+)-free medium the magnitude of depolarization decreased. Our data suggest a contribution of Ca(2+)-activated K+ channels to the hyperpolarizing effect of ATP at lower concentrations. The direction of membrane potential changes is determined presumably by a sensitive balance of ATP-receptor mediated Ca(2+)- and Na(+)-influx and the Ca(2+)-activated K(+)-channel activity.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Adenosine Triphosphate Animal Biophysics Cell Membrane cytology drug effects Hungary Kinetics Membrane Potentials Mice Mice,Inbred BALB C pharmacology physiology Quinine Support,Non-U.S.Gov't Thymus Gland Verapamil
Megjelenés:	Biochemical and Biophysical Research Communications. - 191 : 2 (1993), p. 378-384. -
További szerzők:	Nagy Péter (1971-) (biofizikus) Panyi György (1966-) (biofizikus) Vereb György (1965-) (biofizikus, orvos) Bene László (1963-) (biofizikus) Mátyus László (1956-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus)
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001-es BibID:	BIBFORM006046
Első szerző:	Mátyus László (biofizikus)
Cím:	Voltage gating of Ca2(+)-activated potassium channels in human lymphocytes / Matyus, L., Pieri, C., Recchioni, R., Moroni, F., Bene, L., Tron, L., Damjanovich, S.
Dátum:	1990
Megjegyzések:	The effect of membrane potential on Ca2+ activated K+ channels was studied on human peripheral lymphocytes. Membrane potential was monitored using bisoxonol and flow cytometry. 1 mM Ca2+ in the presence of 2 microM ionomycin depolarized the control cell population, while 100 microM Ca2+ caused hyperpolarization. However 1 mM Ca2+ had a hyperpolarizing effect on previously partially depolarized cells. Potassium channel blockers did not influence the depolarization, while they inhibited the hyperpolarization. Based on the experimental evidence a voltage gating of Ca2+ activated K+ channels is suggested.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Biophysics Calcium drug effects Flow Cytometry Human Hungary In Vitro Ion Channel Gating Ionomycin Leukocytes,Mononuclear Lymphocytes Membrane Potentials pharmacology physiology Potassium Potassium Channel Blockers Potassium Channels Support,Non-U.S.Gov't
Megjelenés:	Biochemical and Biophysical Research Communications. - 171 : 1 (1990), p. 325-329. -
További szerzők:	Pieri, Carlo Recchioni, Rina Moroni, Fausto Bene László (1963-) (biofizikus) Trón Lajos (1941-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus)
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