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1.

001-es BibID:BIBFORM029089
Első szerző:Ádány Róza (megelőző orvostan és népegészségtan szakorvos)
Cím:Factor XIII of blood coagulation as a nuclear crosslinking enzyme / Ádány R., Bárdos H., Antal M., Módis L., Sárváry A., Szűcs S., Balogh I.
Dátum:2001
Megjegyzések:Intracellular localization and distribution of Factor XIII subunit A (FXIIIA) was investigated in association with monocyte-macrophage differentiation in a long term culture of human monocytes by light- and electron microscopical as well as biochemical and immunobiochemical techniques. To allow the detection of FXIIIA in cells with well-preserved ultrustructure, immunosera against glutaraldehyde-derivatized recombinant FXIIIA were developed in rabbits, then characterized and used in this study. In the early phase of macrophage differentiation intranuclear accumulation of FXIIIA was detected as a transient phenomenon in cells of the 2nd day culture by optical sectioning with 0,7 microm steps in laser scanning confocal microscopy and immunoblotting technique. FXIIIA could be detected by immunoelectron microscopic postembedding staining over electrodense DNA-containing areas. Fluoresceinated monodansylcadaverine incorporation assay was used to demonstrate that FXIIIA is not only present in the nuclei, but also expresses its transglutaminase activity. Our finding of the nuclear accumulation of FXIIIA in differentiating human macrophages is also unique in that a blood clotting factor has, for the first time, been localized in nuclei and has been shown to be an intracellular crosslinking enzyme. The possible role of nuclear FXIIIA in association with cellular processes involving chromatin structure remodeling, such as cell death, cell differentiation or cellular proliferation requires further in-depth investigation.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Thrombosis and Haemostasis. - 85 : 5 (2001), p. 845-851. -
További szerzők:Bárdos Helga (1969-) (megelőző orvostan és népegészségtan szakorvos) Antal Miklós (1951-) (orvos, anatómus) Módis László (1939-) (anatómus, kötőszövetbiológus) Sárváry Attila (1971-) (népegészségtan szakorvos) Szűcs Sándor (1958-) (biokémikus, vegyész) Balogh Imre
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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2.

001-es BibID:BIBFORM041241
Első szerző:McKee, Martin
Cím:The Composition of Surrogate Alcohols Consumed in Russia / McKee Martin, Szűcs Sándor, Sárváry Attila, Ádány Róza, Kiryanov Nikolay, Saburova Ludmila, Tomkins Susannah, Andreev Evgeny, Leon David A.
Dátum:2005
ISSN:0145-6008
Megjegyzések:BACKGROUND: In the course of a case-control study examining determinants of premature death among working age men, it became clear that a significant percentage of the population (7.3%) were drinking a variety of surrogate alcohol products (products not legally sold for consumption). In this population, where there is a high death rate from alcohol-related causes, including acute alcohol poisoning, it was important to know what these products contained. METHODS: The identity of products being consumed was identified from the survey of controls. Representative samples were obtained and subjected to analysis using gas chromatography and mass spectrometry to determine their composition. RESULTS: Three broad groups of product were identified: samogon (home-produced spirits); medicinal compounds; and other spirits (mainly sold as aftershaves). Commercially produced vodkas were used for comparison. Samogon contained lower quantities of ethanol than vodka [mean, 39 vs. 44 volumetric percentage (v/v%), respectively] but in addition contained certain toxic long-chain alcohols. Medicinal compounds contained only ethanol, at a higher concentration that vodka (mean, 66 v/v%), while the other spirits, which were also essentially pure ethanol, contained a mean of 94 v/v%. CONCLUSIONS: A significant number of Russian men are drinking products that have either very high concentrations of ethanol or contaminants known to be toxic. These products are untaxed and thus much less expensive than vodka. There is an urgent need for policy responses that target their production and consumption.
Tárgyszavak:Orvostudományok Egészségtudományok idegen nyelvű folyóiratközlemény külföldi lapban
Russia
Surrogate Alcohol
Alcohol Poisoning
Megjelenés:Alcoholism-Clinical And Experimental Research. - 29 : 10 (2005), p. 1884-1888. -
További szerzők:Szűcs Sándor (1958-) (biokémikus, vegyész) Sárváry Attila (1971-) (népegészségtan szakorvos) Ádány Róza (1952-) (megelőző orvostan és népegészségtan szakorvos) Kiryanov, Nikolay Saburova, Ludmila Tomkins, Susannah Andreev, Evgeny Leon, David A.
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DOI
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3.

001-es BibID:BIBFORM006790
Első szerző:Sárváry Attila (népegészségtan szakorvos)
Cím:Possible role of factor XIII subunit A in Fcgamma and complement receptor-mediated phagocytosis / Sarvary, A., Szucs, S., Balogh, I., Becsky, A., Bardos, H., Kavai, M., Seligsohn, U., Egbring, R., Lopaciuk, S., Muszbek, L., Adany, R.
Dátum:2004
ISSN:0008-8749
Megjegyzések:Besides its traditional role in hemostasis, factor XIII subunit A (FXIII-A) is supposed to function as a cellular transglutaminase and to be involved in certain intracellular processes, including cytoskeletal remodeling. To investigate its intracellular role, the aim of the present study was to follow changes in FXIII-A production in combination with the receptor-mediated phagocytic activities of monocytes/macrophages and to examine the phagocytic functions of monocytes in patients with FXIII-A deficiency. Human blood monocytes were isolated from the buffy coats of healthy volunteers and cultured for 4 days. The FcgammaR-mediated phagocytosis of sensitized erythrocytes (EA) and the complement receptor (CR)-mediated phagocytosis of complement-coated yeast particles were studied during monocyte/macrophage differentiation. Changes in the gene expression of FXIII-A were detected by real-time quantitative RT-PCR. FXIII-A protein production was investigated with fluorescent image analysis at single cell level and Western immunoblot analysis. Both the FcgammaR and CR-mediated phagocytosis increased during culturing, which peaked on day 3. The phagocytic activity of the cells could be markedly inhibited with monodansylcadaverine, an inhibitor of the transglutaminase-induced crosslinking of proteins. The phagocytosis of EA, complement-coated and uncoated yeast particles was found to be strongly diminished in monocytes of FXIII-A deficient patients. The phagocytic functions of cultured cells showed a change in parallel with the alterations in FXIII-A mRNA expression, as well as with that in FXIII-A in protein synthesis detected by image and Western immunoblot analyses in concert. Our results suggest that FXIII-A plays a role in the Fcgamma and complement receptor-mediated phagocytic activities of monocytes/macrophages.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Blotting, Western
Cadaverine
Enzyme Inhibitors
Erythrocytes
Factor XIII
Female
Humans
Macrophages
Male
Microscopy, Fluorescence
Phagocytosis
RNA
Receptors, Complement 3b
Receptors, IgG
Reverse Transcriptase Polymerase Chain Reaction
Transglutaminases
Megjelenés:Cellular Immunology. - 228 : 2 (2004), p. 81-90. -
További szerzők:Szűcs Sándor (1958-) (biokémikus, vegyész) Balogh Imre Becsky Áron Bárdos Helga (1969-) (megelőző orvostan és népegészségtan szakorvos) Kávai Mária (1930-) (vegyész) Seligsohn, Uri Egbring, Rudolf Lopaciuk, Stanislaw Muszbek László (1942-) (haematológus, kutató orvos) Ádány Róza (1952-) (megelőző orvostan és népegészségtan szakorvos)
Internet cím:elektronikus változat
DOI
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4.

001-es BibID:BIBFORM042722
Első szerző:Szűcs Sándor (biokémikus, vegyész)
Cím:Single-cell measurement of granulocyte respiratory burst with digital imaging fluorescence microscopy / S. Szűcs, G. Vámosi, R. Póka, A. Sárváry, H. Bárdos, M. Balázs, L. Tóth, J. Kappelmayer, J. Szőllősi, R. Ádány
Dátum:1998
ISSN:0007-1048
Tárgyszavak:Orvostudományok Elméleti orvostudományok idézhető absztrakt
Megjelenés:British Journal of Haematology. - 102 : 1 (1998), p. 2-139. -
További szerzők:Vámosi György (1967-) (biofizikus) Póka Róbert (1960-) (szülész-nőgyógyász, klinikai onkológus) Sárváry Attila (1971-) (népegészségtan szakorvos) Bárdos Helga (1969-) (megelőző orvostan és népegészségtan szakorvos) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Tóth L. Kappelmayer János (1960-) (laboratóriumi szakorvos) Szöllősi János (1953-) (biofizikus) Ádány Róza (1952-) (megelőző orvostan és népegészségtan szakorvos)
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5.

001-es BibID:BIBFORM041289
Első szerző:Szűcs Sándor (biokémikus, vegyész)
Cím:Simultaneous determination of styrene, toluene, and xylene metabolites in urine by gas chromatography/mass spectrometry / Szűcs Sándor, Tóth László, Legoza József, Sárváry Attila, Ádány Róza
Dátum:2002
ISSN:0340-5761
Megjegyzések:Exposure to styrene, toluene, and xylene (STX) frequently occurs in various industrial settings leading to several adverse health effects. Therefore, the biological monitoring by determination of urinary mandelic acid (MA) and phenylglyoxylic acid (PGA), hippuric acid (HA), and 2-, 3-, and 4-methylhippuric acids (2-, 3-, and 4-MHAs), the metabolites of STX, is required or at least recommended in case of workers exposed by these agents. Considering the fact that co-exposure to STX frequently occurs, methods that have been described for the separate analysis of these compounds in urine samples cannot be used effectively for monitoring. Therefore, a reliable gas chromatographic/mass spectrometric (GC/MS) method was developed for the simultaneous identification and quantification of these metabolites. Following solid phase extraction of the urine samples, the extracts were silylated and analyzed by GC/MS using a HP-5MS capillary column. The method was evaluated for linearity, limits of detection and quantification, and specificity, as well as for precision, extraction efficiency, and stability at three different concentrations prepared in urine. The assay was linear up to 0.16 mg/ml for MA, and 0.32 mg/ml for PGA, HA, and 2-, 3- and 4-MHAs. The limits of detection and quantification of STX metabolites varied between 0.001 and 0.02 mg/ml, and from 0.01 to 0.04 mg/ml, respectively. The within-day and between-day precision, determined at low, medium, and high concentrations, ranged from 2 to 12% and 2 to 19%, respectively. The extraction efficiency was 70-80%. No degradation of the metabolites occurred in the urine samples under the possible working conditions. The method was applied for the analysis of the urine samples from exposed workers. The cost- and time-effectiveness, the technical advantages and validity parameters of this GC/MS analysis make it suitable for biological monitoring of mixed exposure to styrene, toluene and xylene.
Tárgyszavak:Orvostudományok Egészségtudományok idegen nyelvű folyóiratközlemény külföldi lapban
Styrene
Toluene
Xylene
Metabolites
Gas chromatography/mass spectrometry
Megjelenés:Archives Of Toxicology. - 76 : 10 (2002), p. 560-569. -
További szerzők:Tóth László (orvos) Legoza József Sárváry Attila (1971-) (népegészségtan szakorvos) Ádány Róza (1952-) (megelőző orvostan és népegészségtan szakorvos)
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6.

001-es BibID:BIBFORM041282
Első szerző:Szűcs Sándor (biokémikus, vegyész)
Cím:Could the high level of cirrhosis in central and eastern Europe be due partly to the quality of alcohol consumed? : an exploratory investigation / Szűcs Sándor, Sárváry Attila, McKee Martin, Ádány Róza
Dátum:2005
ISSN:0965-2140
Megjegyzések:BACKGROUND: The burden of alcohol-related diseases differs widely among countries. Since the 1980s, a band of countries in Central and Eastern Europe have experienced a steep rise in deaths from chronic liver diseases and cirrhosis. A possible risk factor is the consumption of illegally produced home-made spirits in these countries containing varying amounts of aliphatic alcohols and which may be hepatotoxic. However, little is known about the composition of such beverages. AIMS: To compare the concentration of short-chain aliphatic alcohols in spirits from illegal and legal sources in Hungary. DESIGN: Samples taken from commercial retailers and illegal sources were collected and their aliphatic patterns and alcohol concentrations were determined by gas chromatographic/mass spectrometric (GC/MS) analysis. FINDINGS: The concentrations of methanol, isobutanol, 1-propanol, 1-butanol, 2-butanol and isoamyl alcohol were significantly higher in home-made spirits than those of from commercial sources. CONCLUSIONS: The results suggest that the consumption of home-made spirits is an additional risk factor for the development of alcohol-induced cirrhosis and may have contributed to high level of liver cirrhosis mortality in Central and Eastern Europe. Restrictions on supply and sale of alcohol from illicit sources are needed urgently to reduce significantly the mortality from chronic liver disease.
Tárgyszavak:Orvostudományok Egészségtudományok idegen nyelvű folyóiratközlemény külföldi lapban
alcohol consumption
cirrhosis
quality of spirits
Megjelenés:Addiction. - 100 : 4 (2005), p. 536-542. -
További szerzők:Sárváry Attila (1971-) (népegészségtan szakorvos) McKee, Martin Ádány Róza (1952-) (megelőző orvostan és népegészségtan szakorvos)
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7.

001-es BibID:BIBFORM027791
Első szerző:Szűcs Sándor (biokémikus, vegyész)
Cím:Method validation for the simultaneous determination of fecal sterols in surface waters by gas chromatography-mass spectrometry / Sándor Szucs, Attila Sárváry, Terry Cain, Róza Ádány
Dátum:2006
Megjegyzések:Besides microbiological methods, fecal pollution of surface waters is estimated by gas chromatographic (GC) determination of sterols present in human and animal sewage effluents. The most frequently used biomarkers for the evaluation of contamination levels include coprostanol, cholesterol, dihydrocholesterol, stigmasterol, beta-sitosterol, and stigmastanol. Although several GC techniques are used to measure these compounds in aquatic systems, the analytical performance of GC-mass spectrometric (MS) determination of these sterols has not been systematically characterized. Therefore, the aim of this work is to validate a simple and rapid GC-MS method for the simultaneous analysis of six sterols, considering all parameters and requirements defined by Good Laboratory Practice. Following liquid-liquid extraction of spiked surface water samples, the extracts are silylated and analyzed by GC-MS. The method is evaluated for linearity and limits of detection and quantitation, as well as for precision, extraction efficiency, and stability. The assay is linear up to 160 ng; the limits of detection and quantitation are 5-10 ng and 20 ng, respectively. The within- and between-day precision ranged from 1% to 9% and 1% to 16%, respectively. The extraction efficiency was 65-80%. The stability studies indicate that the sterols in surface water samples begin to degrade after 24 h of refrigerated storage. However, three freeze/thaw cycles could be performed without their decomposition. The method is applied to the analysis of surface water and wastewater samples. The technical advantages make this GC-MS analysis suitable for routine environmental monitoring of fecal pollution in aquatic systems.
Tárgyszavak:Orvostudományok Egészségtudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Journal of Chromatographic Science. - 44 : 2 (2006), p. 70-76. -
További szerzők:Sárváry Attila (1971-) (népegészségtan szakorvos) Cain, Terry Ádány Róza (1952-) (megelőző orvostan és népegészségtan szakorvos)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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8.

001-es BibID:BIBFORM004640
Első szerző:Szűcs Sándor (biokémikus, vegyész)
Cím:Single-cell measurement of superoxide anion and hydrogen peroxide production by human neutrophils with digital imaging fluorescence microscopy / Sándor Szűcs, György Vámosi, Róbert Póka, Attila Sárváry, Helga Bárdos, Margit Balázs, János Kappelmayer, László Tóth, János Szöllősi, Róza Ádány
Dátum:1998
ISSN:0196-4763
Megjegyzések:Besides flow cytometry, fluorescence microscopy combined with computerized image analysis offers an alternative tool for assessing phagocyte oxidant generation at the single-cell level. This technique provides an opportunity for the direct visualization of cells and simultaneous measurement of cellular fluorescence intensity. Thus, we developed a simple method for the quantitative evaluation of intracellular superoxide anion and hydrogen peroxide production with image cytometry by using hydroethidine and dihydrorhodamine 123 dyes, respectively. Human neutrophils stimulated with phorbol dibutyrate and labeled by these fluorogenic substrates showed intense, well recognizable red or green fluorescence. The intensity of signals from individual granulocytes of cytospin preparations were quantitatively measured in digitized images. There was a great heterogeneity in response to the stimulus within the granulocyte population as shown by the integrated fluorescence intensity values. In agreement with the results of parallel flow cytometric experiments, this simple image analysis performed on cells of cytospin preparations was able to detect the defects in the oxidative metabolism of neutrophils from patients with cervix carcinoma. We demonstrated that even minor alterations in superoxide anion/hydrogen peroxide generation can be detected by image cytometry as efficiently as by flow cytometry. This result validates imaging microscopy as an alternative to flow cytometry in such experiments. In addition, the image cytometric technique allows the observation of the kinetics of free radical production in individual cell under adherent conditions. Therefore, we carried out image analysis of the oxidative burst of neutrophils adherent to uncoated glass and fibronectin- and type IV collagen-coated surfaces in response to stimulation with phorbol dibutyrate or N-formyl-methionyl-leucyl-phenylalanine. We elaborated a calibration technique for the quantitative measurement of the ethidium bromide generation mediated by superoxide anion within individual adherent granulocytes. The ethidium bromide production varied between 0.48 and 1.17 amol/cell/min.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
analysis
Anions
Carcinoma
Dyes
epidemiology
Ethidium
Flow Cytometry
Fluorescence
Human
Hungary
Hydrogen Peroxide
Image Cytometry
Image Processing
Computer-Assisted
Kinetics
metabolism
methods
Microscopy
Microscopy
Fluorescence
Neutrophils
Superoxides
Support
Non-U.S.Gov't
egyetemen (Magyarországon) készült közlemény
Megjelenés:Cytometry. - 33 : 1 (1998), p. 19-31. -
További szerzők:Vámosi György (1967-) (biofizikus) Póka Róbert (1960-) (szülész-nőgyógyász, klinikai onkológus) Sárváry Attila (1971-) (népegészségtan szakorvos) Bárdos Helga (1969-) (megelőző orvostan és népegészségtan szakorvos) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Kappelmayer János (1960-) (laboratóriumi szakorvos) Tóth László (orvos) Szöllősi János (1953-) (biofizikus) Ádány Róza (1952-) (megelőző orvostan és népegészségtan szakorvos)
Internet cím:DOI
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