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001-es BibID:	BIBFORM020764
Első szerző:	Kis-Tóth Katalin (immunológus)
Cím:	Voltage-Gated Sodium Channel Nav1.7 Maintains the Membrane Potential and Regulates the Activation and Chemokine-Induced Migration of a Monocyte-Derived Dendritic Cell Subset / Katalin Kis-Toth, Peter Hajdu, Ildiko Bacskai, Orsolya Szilagyi, Ferenc Papp, Attila Szanto, Edit Posta, Peter Gogolak, Gyorgy Panyi, Eva Rajnavolgyi
Dátum:	2011
Megjegyzések:	Expression of CD1a protein defines a human dendritic cell (DC) subset with unique functional activities. We aimed to study the expression of the Nav1.7 sodium channel and the functional consequences of its activity in CD1a(-) and CD1a(+) DC. Single-cell electrophysiology (patch-clamp) and quantitative PCR experiments performed on sorted CD1a(-) and CD1a(+) immature DC (IDC) showed that the frequency of cells expressing Na(+) current, current density, and the relative expression of the SCN9A gene encoding Nav1.7 were significantly higher in CD1a(+) cells than in their CD1a(-) counterparts. The activity of Nav1.7 results in a depolarized resting membrane potential (-8.7 +/- 1.5 mV) in CD1a(+) IDC as compared with CD1a(-) cells lacking Nav1.7 (-47 +/- 6.2 mV). Stimulation of DC by inflammatory signals or by increased intracellular Ca(2+) levels resulted in reduced Nav1.7 expression. Silencing of the SCN9A gene shifted the membrane potential to a hyperpolarizing direction in CD1a(+) IDC, resulting in decreased cell migration, whereas pharmacological inhibition of Nav1.7 by tetrodotoxin sensitized the cells for activation signals. Fine-tuning of IDC functions by a voltage-gated sodium channel emerges as a new regulatory mechanism modulating the migration and cytokine responses of these DC subsets
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban ACTIVATION article Cells Electrophysiology Human Hungary immunology Sodium Tetrodotoxin
Megjelenés:	The Journal of Immunology. - 187 : 3 (2011), p. 1273-1280. -
További szerzők:	Hajdu Péter (1975-) (biofizikus) Bacskai Ildikó (1985-) (immunológus) Szilágyi Orsolya (1985-) (molekuláris biológus, biokémikus) Papp Ferenc (1979-) (biofizikus) Szántó Attila (1976-) (orvos, biokémikus) Feketéné Posta Edit (1986-) (reumatológus) Gogolák Péter (1968-) (biológus, immunológus) Panyi György (1966-) (biofizikus) Rajnavölgyi Éva (1950-) (immunológus)
Pályázati támogatás:	TÁMOP-4.2.1/B-09/1/KONV-2010-0007 TÁMOP Molekuláris immunológia TÁMOP-4.2.2-08/1-2008-0015 TÁMOP
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat Szerző által megadott URL
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001-es BibID:	BIBFORM037603
Első szerző:	Varga Zoltán (biofizikus, szakfordító)
Cím:	Potassium channel expression in human CD4(+) regulatory and naive T cells from healthy subjects and multiple sclerosis patients / Zoltan Varga, Tunde Csepany, Ferenc Papp, Akos Fabian, Peter Gogolak, Agnes Toth, Gyorgy Panyi
Dátum:	2009
ISSN:	0165-2478
Megjegyzések:	The membrane potential of human T cells is regulated by two potassium channels: the voltage-gatedKV1.3 and the Ca2+-activated KCa3.1. These two channels are essential for efficient antigenic activation andproliferation of T cells and are expressedat different levels in naïve, centralmemory and effectormemory Tcells. This provides the opportunity to inhibit the proliferation of the targeted subtype by channel-specificblocking compounds. Regulatory T cells (Tregs) also represent a unique subtype of T cells that performhighly specialized tasks in controlling immune responses, which raises the possibility that they too havea distinctive channel expression pattern. Using whole-cell patch-clamp we tested this hypothesis anddetermined the ion channel expression of CD4+CD25hiCD127lo regulatory and CD4+CD25loCD127hi naïveT cells from the peripheral blood of healthy volunteers and multiple sclerosis (MS) patients sorted by flowcytometry. We have found that naïve and Treg cells from healthy controls expressed equal numbers ofKV1.3 channels, while Tregs had a greater membrane surface as assessed by capacitance measurements,and consequentially lower channel density than naïve cells, indicating an "incomplete activation state"of Tregs. In contrast, Tregs from MS patients had fewer KV1.3 channels than naïve cells and there wasno difference in the membrane capacitance or channel density between the two subtypes of cells. Theexpression level of KCa3.1 channels was similar in all cell subsets. The observed differences in KV1.3channel expression density may contribute to the varying responses upon antigenic stimulation by thesecell types in health and disease.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Regulatory T cell Potassium channel Multiple sclerosis Cell capacitance Channel density egyetemen (Magyarországon) készült közlemény
Megjelenés:	Immunology Letters 124 : 2 (2009), p. 95-101. -
További szerzők:	Csépány Tünde (1956-) (neurológus, pszichiáter) Papp Ferenc (1979-) (biofizikus) Fábián Ákos István (1982-) (aneszteziológus) Gogolák Péter (1968-) (biológus, immunológus) Tóth Ágnes (1983-) (biofizikus) Panyi György (1966-) (biofizikus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat Szerző által megadott URL DOI
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