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001-es BibID:BIBFORM004837
Első szerző:Hajas György (biológus)
Cím:New phenotypic, functional and electrophysiological characteristics of KG-1 cells / György Hajas, Emese Zsiros, Tünde László, Péter Hajdú, Sándor Somodi, Bence Réthi, Péter Gogolák, Katalin Ludányi, György Panyi, Éva Rajnavölgyi
Dátum:2004
Megjegyzések:Myeloid dendritic cells (DC) are representatives of a rare and phenotypically diverse population of professional antigen presenting cells possessing high functional heterogeneity and flexibility. Here we studied the phenotypic, functional and electrophysiological characteristics of KG-1 cells, an erythroleukemia model cell line, which shares morphological and physiological similarities with immature and mature myeloid DC. We compared the expression of internalizing receptors and other cell surface molecules, antigen uptake and migration of unstimulated and activated KG-1 cells with the characteristics of immature and mature DC. Unstimulated KG-1 cells were less potent in capturing extracellular materials than immature DC. In contrast to monocyte-derived DC KG-1 cells stimulated by PMA and ionomycin ceased to migrate along the MIP-3beta chemokine gradient despite their high expression of CCR7 chemokine receptor and MDR, a transporter implicated in DC migration. Moreover, we determined the ion channel repertoire of KG-1 cells before and after treatment with PMA and ionomycin by using the patch-clamp technique. We found that both unstimulated and activated KG-1 cells expressed time- and voltage-independent, ChTx sensitive intracellular Ca(2+)-gated potassium conductance suggesting the presence of K(Ca) channels in their membranes. Based on our results we propose that KG-1 cells resemble myeloid DC but also possess unique phenotypic, functional and electrophysiological characteristics.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
analogs and derivatives
Animals
Calcium
Cell Line
Cell Movement
Cells
Dendritic Cells
Dextrans
Fluorescein
Fluorescein-5-isothiocyanate
Humans
Hungary
immunology
Ionomycin
Isoquinolines
Leukemia,Erythroblastic,Acute
metabolism
Patch-Clamp Techniques
physiology
Potassium
Research
Support
Tumor Cells,Cultured
Megjelenés:Immunology Letters. - 92 : 1-2 (2004), p. 97-106. -
További szerzők:Zsíros Emese (1980-) (orvos) László Tünde Hajdu Péter (1975-) (biofizikus) Somodi Sándor (1977-) (belgyógyász) Réthi Bence (1973-) (biológus, immunológus) Gogolák Péter (1968-) (biológus, immunológus) Ludányi Katalin (1975-) (immunológus) Panyi György (1966-) (biofizikus) Rajnavölgyi Éva (1950-) (immunológus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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2.

001-es BibID:BIBFORM062512
Első szerző:Pethő Zoltán (orvos)
Cím:The anti-proliferative effect of cation channel blockers in T lymphocytes depends on the strength of mitogenic stimulation / Zoltan Petho, Andras Balajthy, Adam Bartok, Krisztian Bene, Sandor Somodi, Orsolya Szilagyi, Eva Rajnavolgyi, Gyorgy Panyi, Zoltan Varga
Dátum:2016
ISSN:0165-2478
Megjegyzések:Ion channels are crucially important for the activation and proliferation of T lymphocytes, and thus, for the function of the immune system. Previous studies on the effects of channel blockers on T cell proliferation reported variable effectiveness due to differing experimental systems. Therefore our aim was to investigate how the strength of the mitogenic stimulation influences the efficiency of cation channel blockers in inhibiting activation, cytokine secretion and proliferation of T cells under standardized conditions. Human peripheral blood lymphocytes were activated via monoclonal antibodies targeting the TCR-CD3 complex and the co-stimulator CD28. We applied the blockers of Kv1.3 (Anuroctoxin), KCa3.1 (TRAM-34) and CRAC (2-Apb) channels of T cells either alone or in combination with rapamycin, the inhibitor of the mammalian target of rapamycin (mTOR). Five days after the stimulation ELISA and flow cytometric measurements were performed to determine IL-10 and IFN-? secretion, cellular viability and proliferation. Our results showed that ion channel blockers and rapamycin inhibit IL-10 and IFN-? secretion and cell division in a dose-dependent manner. Simultaneous application of the blockers for each channel along with rapamycin was the most effective, indicating synergy among the various activation pathways. Upon increasing the extent of mitogenic stimulation the anti-proliferative effect of the ion channel blockers diminished. This phenomenon may be important in understanding the fine-tuning of T cell activation.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Immune regulation
Rapamycin
ion channel
Cytokine secretion
T cells
Megjelenés:Immunology Letters 171 (2016), p. 60-69. -
További szerzők:Balajthy András (1988-) (általános orvos) Bartók Ádám (1984-) (biotechnológus) Bene Krisztián (1986-) (Biológus) Somodi Sándor (1977-) (belgyógyász) Szilágyi Orsolya (1985-) (molekuláris biológus, biokémikus) Rajnavölgyi Éva (1950-) (immunológus) Panyi György (1966-) (biofizikus) Varga Zoltán (1969-) (biofizikus, szakfordító)
Pályázati támogatás:KTIA NAP 13-2-2015-0009
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Internet cím:Szerző által megadott URL
DOI
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