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001-es BibID:	BIBFORM042654
035-os BibID:	(scopus)19444380683 (wos)000229353200007
Első szerző:	Horváth Gábor (biofizikus)
Cím:	Selecting the right fluorophores and flow cytometer for fluorescence resonance energy transfer measurements / Gábor Horváth, Miklós Petrás, Gergely Szentesi, Ákos Fábián, John W. Park, György Vereb, János Szöllősi
Dátum:	2005
ISSN:	1552-4922 1552-4930
Megjegyzések:	BACKGROUND:Fluorescence resonance energy transfer applied in flow cytometry (FCET) is an excellent tool for determining supramolecular organization of biomolecules at the cell surface or inside the cell. Availability of new fluorophores and cytometers requires the establishment of fluorophore dye pairs most suitable for FCET measurements.METHODS:A gastric tumor cell line (N87) was labeled for major histocompatibility complex class I heavy chain and beta2-microglobulin with antibodies conjugated with fluorescein- and indocarbocyanine-like fluorophores and analyzed in FCET measurements on a cell-by-cell basis using three flow cytometers: FACSCalibur, FACSDiVa, and FACSArray.RESULTS:Normalized fluorescence intensity values were measured and normalized energy transfer efficiencies, spectral overlap integrals, and crucial dye- and instrument-dependent parameters were calculated for all matching pairs of seven fluorophores on the three commercial cytometers. The most crucial parameter in determining the applicability of the donor-acceptor pairs was the normalized fluorescence intensity and the least important one was the spectral overlap.CONCLUSIONS:On the basis of available laser lines, the optimal dye pair for all three cytometers is the Alexa546-Alexa647 pair, which produces high energy transfer efficiency values and has the best spectral characteristics with regard to laser excitation, detection of emission, and spectral overlap.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk fluorescence resonance energy transfer flow cytometry donor-acceptor pair egyetemen (magyarországon) készült közlemény
Megjelenés:	Cytometry. Part A. - 65A : 2 (2005), p. 148-157. -
További szerzők:	Petrás Miklós (1977-) (orvos) Szentesi Gergely (1976-) (kémia-fizika tanár) Fábián Ákos István (1982-) (aneszteziológus) Park, John W. Vereb György (1965-) (biofizikus, orvos) Szöllősi János (1953-) (biofizikus)
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001-es BibID:	BIBFORM042484
035-os BibID:	(scopus)23844475901 (wos)000231845600013
Első szerző:	Mocanu, Maria-Magdalena
Cím:	Associations of ErbB2, beta1-integrin and lipid rafts on Herceptin (Trastuzumab) resistant and sensitive tumor cell lines / Maria-Magdalena Mocanu, Zsolt Fazekas, Miklós Petrás, Péter Nagy, Zsolt Sebestyén, Jorma Isola, József Tímár, John W. Park, György Vereb, János Szöllősi
Dátum:	2005
ISSN:	0304-3835
Megjegyzések:	ErbB2-mediated transmembrane signaling is a key target of novel anticancer agents such as Herceptin. Our comparison of Herceptin resistant (JIMT-1, MKN-7) and sensitive (SKBR-3, N-87) cell lines demonstrates the importance of ErbB2 association patterns involving integrins and lipid rafts. Flow cytometric FRET and confocal microscopic measurements revealed colocalization and molecular proximity between b1-integrins and ErbB2, as well as their association with lipid rafts. A weak functional interaction between ErbB2 and b1-integrin and the fact that ErbB2 did not co-patch with b1-integrins uponcrosslinking imply that ErbB2 and b1-integrin define two distinct molecular association clusters from a functional point of view. Although Herceptin-sensitive cell lines expressed more ErbB2 and fewer b1-integrin molecules on their surface than their resistant counterparts, this finding probably does not explain the Herceptin resistant phenotype due to the weak interaction between b1-integrins and ErbB2. Our results imply that the true significance of the expression profile of proteins involved inoncogenesis can only be understood after characterizing their molecular interactions.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Antibodies Antibodies,Monoclonal Antigens,CD29 Biophysics Breast Neoplasms Cell Line Cell Membrane Drug Resistance,Neoplasm Humans Hungary Membrane Microdomains metabolism pathology pharmacology Phenotype physiology Proteins Receptor,erbB-2 Research Support Tumor Cells,Cultured egyetemen (Magyarországon) készült közlemény
Megjelenés:	Cancer Letters. - 227 : 2 (2005), p. 201-212. -
További szerzők:	Fazekas Zsolt (1971-) (biofizikus) Petrás Miklós (1977-) (orvos) Nagy Péter (1971-) (biofizikus) Sebestyén Zsolt Isola, Jorma Timár József Park, John W. Vereb György (1965-) (biofizikus, orvos) Szöllősi János (1953-) (biofizikus)
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