CCL

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001-es BibID:BIBFORM018657
035-os BibID:(WoS)000275379300019 (Scopus)77949790049
Első szerző:Memboeuf, Antony
Cím:Size Effect on Fragmentation in Tandem Mass Spectrometry / Antony Memboeuf, Andreas Nasioudis, Sergio Indelicato, Ferenc Pollreisz, Ákos Kuki, Sándor Kéki, Oscar F. van den Brink, Károly Vékey, László Drahos
Dátum:2010
ISSN:0003-2700
Megjegyzések:The collision energy or collision voltage necessary to obtain 50% fragmentation (characteristic collision energy/voltage, CCE or CCV) has been systematically determined for different types of molecules [poly(ethylene glycols) (PEG), poly(tetrahydrofuran) (PTHF), and peptides] over a wide mass (degrees of freedom) range. In the case of lithium-cationized PEGs a clear linear correlation (R(2) > 0.996) has been found between CCE and precursor ion mass on various instrument types up to 4.5 kDa. A similar linear correlation was observed between CCV and the mass-to-charge ratio. For singly and multiply charged polymers studied under a variety of experimental conditions and on several instruments, all data were plotted together and showed correlation coefficient R(2) = 0.991. A prerequisite to observe such a good linear correlation is that the energy and entropy of activation in a class of polymers is likely to remain constant. When compounds of different structure are compared, the CCV will depend not only on the molecular mass but the activation energy and entropy as well. This finding has both theoretical and practical importance. From a theoretical point of view it suggests fast energy randomization up to at least 4.5 kDa so that statistical rate theories are applicable in this range. These results also suggest an easy method for instrument tuning for high-throughput structural characterization through tandem MS: after a standard compound is measured, the optimum excitation voltage is in a simple proportion with the mass of any structurally similar analyte at constant experimental conditions.
Tárgyszavak:Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Surface-induced dissiciation
Protonated leucine-enkephalin
Electron-capture dissociation
Polyethylene-glycol
Internal energy
Collisional activation
Gas-phase
Covalent attachment
Nonergodic process
Small peptides
Megjelenés:Analytical Chemistry. - 82 : 6 (2010), p. 2294-2302. -
További szerzők:Nasioudis, Andreas Indelicato, Sergio Pollreisz Ferenc Kuki Ákos (1966-) (villamosmérnök) Kéki Sándor (1964-) (polimer kémikus) Brink, Oscar F. van den Vékey Károly Drahos László
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