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1.

001-es BibID:BIBFORM041173
Első szerző:Burch, Henry B.
Cím:Nucleotide and amino acid homology between the human thyrotropin receptor and the HIV-1 nef protein: Identification and functional analysis / Burch Henry B., Nagy Endre V., Lukes Yvonne G., Wartofsky Leonard, Burman Kenneth D.
Dátum:1991
ISSN:0006-291X
Megjegyzések:A comparison of the nucleotide sequence of the human thyrotropin receptor (hTSH-R) with that of HIV-1 revealed 61% homology between a 161 base pair region encoding a unique portion of the hTSH-R and an immunogenic HIV-1 regulatory protein, nef. Amino acid analysis of this region shows 27% homology, including a segment in which 7/10 consecutive amino acids are identical. Sera from rabbits successfully immunized with a 16 amino acid portion of the hTSH-R (352-367, p1) was assessed for reactivity against a partially homologous nef peptide (nef-1) by ELISA, with a finding five-fold higher post-immunization values compared to pre-immune sera. The specificity of this response was verified with Western blot, using recombinant nef protein. An ELISA using nef-1 gave 64% higher values with sera from Graves' disease patients than with normal controls. This homology and immunologic cross-reactivity suggests an avenue through which a shared immune response against an HIV-1 related retrovirus could play a role in the pathogenesis of Graves' disease.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Biochemical And Biophysical Research Communications. - 181 : 1 (1991), p. 498-505. -
További szerzők:Nagy Endre V. (1957-) (belgyógyász, endokrinológus) Lukes, Yvonne G. Wartofsky, Leonard Burman, Kenneth D.
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2.

001-es BibID:BIBFORM041176
Első szerző:Burch, Henry B.
Cím:Expression polymerase chain reaction for the in vitro synthesis and epitope mapping of autoantigen application to the human thyrotropin receptor / Burch Henry B., Nagy Endre V., Kain Kevin C., Lanar David E., Carr Frances E., Wartofsky Leonard, Burman Kenneth D.
Dátum:1993
ISSN:0022-1759
Megjegyzések:The clinical applicability of a newly described polymerase chain reaction directed protein expression system was assessed for the in vitro synthesis and partial epitope mapping of large radiolabeled human thyrotropin receptor (hTSH-R) protein segments. PCR amplification of targeted regions within the hTSH-R cDNA followed by in vitro transcription and translation permitted rapid synthesis of protein segments ranging in size from 18 to 62 kDa. Initial epitope mapping was directed at a 52 amino acid segment unique to the hTSH-R compared to otherwise homologous glycoprotein hormone receptors. Sera from Graves' disease patients known to have autoantibodies against the hTSH-R were used to immunoprecipitate two protein fragments differing only by the presence of the unique region in the larger fragment (E5) but not in the smaller fragment (E4). Dense precipitation bands were obtained using Graves' sera to immunoprecipitate E5 whereas little or no specific immunoprecipitation of E4 occurred. Normal sera gave only weak immunoprecipitation bands of E5. The technique provides significant advantages over conventional cloning methods and should have general applicability in the study of other protein targets of autoimmune disease.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Journal Of Immunological Methods. - 158 : 1 (1993), p. 123-130. -
További szerzők:Nagy Endre V. (1957-) (belgyógyász, endokrinológus) Kain, Kevin C. Lanar, David E. Carr, Frances E. Wartofsky, Leonard Burman, Kenneth D.
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3.

001-es BibID:BIBFORM041177
Első szerző:Burch, Henry B.
Cím:Thyrotropin receptor antisera for the detection of immunoreactive protein species in retroocular fibroblasts obtained from patients with Graves' ophthalmopathy / Burch, H. B., Sellitti, D., Barnes, S. G., Nagy, E. V., Bahn, R. S., Burman, K. D.
Dátum:1994
ISSN:0021-972X
Megjegyzések:Autoimmunity against the TSH receptor (hTSH-R) is known to be the proximate cause of thyroidal activation in Graves' disease, but has not been definitively linked to extrathyroidal manifestations of this disorder, such as ophthalmopathy and pretibial myxedema. In an effort to increase our knowledge concerning mechanisms responsible for Graves' ophthalmopathy, we used antiserum directed against a highly immunogenic portion of the hTSH-R (amino acids 352-367; P1) to assess the presence of this receptor or immunologically related protein in cultured human retroocular fibroblasts obtained from patients with Graves' ophthalmopathy. Immunoenzymatic and immunofluorescent studies revealed specific staining of both cytoplasmic and cell membrane-associated protein in discrete vesicles. To further evaluate the immunoreactive species present in these cells, immunoblotting experiments were performed using hTSH-R-specific antisera (anti-P1) and sera obtained from patients with Graves' disease. Several protein bands were identified using both anti-P1 and Graves' disease patient sera, including species at mol wt of 95, 71, and 18 kilodaltons, the possible significance of which is discussed. The results support the hypothesis that immunity against the hTSH-R or related proteins contributes to the ophthalmopathy of Graves' disease.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Journal Of Clinical Endocrinology & Metabolism. - 78 : 6 (1994), p. 1384-1391. -
További szerzők:Sellitti, D. Barnes, Stephen Nagy Endre V. (1957-) (belgyógyász, endokrinológus) Bahn, R. S. Burman, Kenneth D.
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4.

001-es BibID:BIBFORM041186
Első szerző:Burch, Henry B.
Cím:Immunodetection of manganese superoxide dismutase in cultured human retroocular fibroblasts using sera directed against the thyrotropin receptor / Burch, H. B., Barnes, S., Nagy, E. V., Sellitti, D., Burman, K. D., Bahn, R. S., Lahiri, S.
Dátum:1998
ISSN:0391-4097
Megjegyzések:The identification of antigenic targets in the retroocular autoimmune response of Graves' ophthalmopathy is likely to increase our understanding of mechanisms underlying this disorder. While a number of putative autoantigens have been identified on the basis of molecular weight or cell of origin, a determination of the significance of these antigens is contingent upon an identification of the amino acid sequence. Our group has previously identified immunoreactive retroocular fibroblast (ROF) proteins recognized by thyrotropin receptor (hTSH-R) antisera (anti-p1), at molecular weights of 95, 71, 41, and 14-25 kDa. In the present study, proteins detected by anti-p1 and visualized by Ponceau staining were isolated and processed for microsequencing. Ponceau staining revealed dense bands at molecular weights of 14 and 23 kDa, and a weak band at 41 kDa. N-terminal sequencing was performed on the prominent band at approximately 23 kDa, showing it to be manganese superoxide dismutase (MnSOD), a mitochondrial enzyme responsible for protection against oxygen free radical-associated cellular damage. Sequence comparison of MnSOD to the hTSH-R peptide, p1, revealed a linear segment of amino acid homology. Preincubation of anti-p1 with p1 blocked immunodetection of the 23 kDa band corresponding to MnSOD, and immunoprecipitation of ROF protein using anti-pi yielded protein recognized by anti-MnSOD. Autoimmunity against human recombinant MnSOD was further assessed by ELISA. Patients with Graves' disease (n = 53) had significantly higher ELISA indices than normal control subjects (n = 29), while patients with Hashimoto's thyroiditis had intermediate values. These results document MnSOD autoantibodies in patients with Graves' disease and suggest that this may result from an immune cross-reactivity between MnSOD and the TSH-receptor.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Journal Of Endocrinological Investigation. - 21 : 1 (1998), p. 48-55. -
További szerzők:Barnes, Stephen Nagy Endre V. (1957-) (belgyógyász, endokrinológus) Sellitti, D. Burman, Kenneth D. Bahn, R. S. Lahiri, Susanta
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5.

001-es BibID:BIBFORM019257
Első szerző:Galuska László (belgyógyász, izotópdiagnoszta)
Cím:Differences in SPET analysis of thyroid-associated orbitopathy / László Galuska, József Varga, Zsolt Szücs-Farkas, Endre V. Nagy, Kenneth D. Burman
Dátum:2004
ISSN:1619-7070
Tárgyszavak:Orvostudományok Klinikai orvostudományok szerkesztői levél
egyetemen (Magyarországon) készült közlemény
Megjelenés:European Journal Of Nuclear Medicine And Molecular Imaging 31 : 5 (2004), p. 793-795. -
További szerzők:Varga József (1955-) (fizikus) Szűcs-Farkas Zsolt Nagy Endre V. (1957-) (belgyógyász, endokrinológus) Burman, Kenneth D.
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6.

001-es BibID:BIBFORM017859
Első szerző:Gazdag Annamária (belgyógyász)
Cím:Improved endothelial function and lipid profile compensate for impaired hemostatic and inflammatory status in iatrogenic chronic subclinical hyperthyroidism of thyroid cancer patients on L-t4 therapy / Gazdag A., Nagy E. V., Burman K. D., Paragh Gy., Jenei Z.
Dátum:2010
Megjegyzések:We aimed to compare the changes of endothelial function and haemostatic, inflammatory and metabolic parameters of short-term iatrogenic hypothyroidism to the characteristics of subclinical hyperthyroidism in patients with differentiated thyroid cancer. DESIGN: Twenty four women (mean age 42.4+/-8.1 years) had undergone total thyroidectomy and radioiodine ablation in treatment for differentiated thyroid cancer. We measured serum thyroglobulin, thyroid function, plasma levels of lipid parameters, homocystine, C-reactive protein, fibrinogen, von Willebrandt factor activity (vWF), nitric oxide, as well as flow-mediated vasodilatation (FMD) and nitroglycerin-mediated vasodilatation of the brachial artery during iatrogenic hypothyroidism (TSH 89.82+/-29.36 mU/L) and again in the same patients during subclinical hyperthyroidism secondary to exogenous levothyroxine administration (TSH 0.24+/-0.11 mU/L). RESULTS: In hypothyroidism, FMD was markedly lower than in subclinical hyperthyroidism (6.79+/-4.44 vs. 14.37+/-8.33%, p<0.005). Total cholesterol (7.34+/-1.23 vs. 4.75+/-1.14 mmol/L, p<0.001), LDL-cholesterol (4.55+/-1.10 vs. 2.70+/-0.89 mmol/L, p<0.005) and homocystine (12.95+/-4.49 vs. 9.62+/-2.29 micromol/L, p<0.005) were significantly higher in hypothyroidism. There was no difference in nitroglycerin-mediated vasodilatation, blood pressure, serum triglyceride and HDL-cholesterol levels according to thyroid function. Fibrinogen (3.23+/-0.50 vs. 4.01+/-0.84 g/L, p<0.005), vWF (90.09+/-25.92 vs.130.63+/-29.97%, p<0.001), C-reactive protein (4.39+/-5.16 vs. 5.55+/-5.15 mg/L, p<0.001) and plasma nitric oxide (24.56+/-6.71 vs. 32.34+/-7.0 micromol/L, <0.005) values were significantly lower in hypothyroidism. FMD correlated in a positive manner with fibrinogen, vWF and nitrogen oxide. CONCLUSIONS: Chronic subclinical hyperthyroidism was associated with improved endothelial function and lipid profile, while haemostatic and inflammatory parameters were impaired. The two opposite mechanisms may well compensate for each other at the level of the vessel wall.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Experimental and Clinical Endocrinology & Diabetes 118 : 6 (2010), p. 381-387. -
További szerzők:Nagy Endre V. (1957-) (belgyógyász, endokrinológus) Burman, Kenneth D. Paragh György (1953-) (belgyógyász) Jenei Zoltán (1968-) (belgyógyász)
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7.

001-es BibID:BIBFORM041178
Első szerző:Mengistu, M.
Cím:TSH receptor gene expression in retroocular fibroblasts / Mengistu, M., Lukes, Y. G., Nagy, V. E., Burch, H. B., Carr, F. E., Lahiri, S., Burman, K. D.
Dátum:1994
ISSN:0391-4097
Megjegyzések:RNA was isolated from fibroblasts from the retroocular area, from endomysial fibroblasts obtained from orbital lateral rectus muscle, and from abdominal skin fibroblasts. The RNA was reverse transcribed into cDNA which was then used as a template for PCR with primers encompassing a portion (nucleotides 989-1235) of the extra-cellular domain of the human TSH receptor (hTSH-R). A definite 247 BP product was detected from fibroblast RNA by ethidium bromide staining, and was confirmed by hybridization with labelled hTSH-R cDNA. The product had homology with the known TSH-R cDNA. These studies indicate that human fibroblasts can express hTSH-R, and they suggest that a cross reactive immunologic response between anti-hTSH-R and these fibroblast TSH receptors may play a role in the genesis of Graves' ophthalmopathy.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Journal Of Endocrinological Investigation. - 17 : 6 (1994), p. 437-441. -
További szerzők:Lukes, Yvonne G. Nagy Endre V. (1957-) (belgyógyász, endokrinológus) Burch, Henry B. Carr, Frances E. Lahiri, Susanta Burman, Kenneth D.
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8.

001-es BibID:BIBFORM041290
035-os BibID:PMID:7901266
Első szerző:Nagy Endre V. (belgyógyász, endokrinológus)
Cím:Immunogenicity of a unique region of the human thyrotropin receptor / E. V. Nagy, H. B. Burch, Y. G. Lukes, F. E. Carr, S. Kosugi, L. D. Kohn, K. D. Burman
Dátum:1993
ISSN:0391-4097
Megjegyzések:Clarifying the role of the TSH receptor protein in the autoimmune process may be the key to understanding the development of Graves' disease. In the present study we used a 16 amino acid peptide of the human TSH receptor (hTSHR) to immunize rabbits. A comparable, but theoretically less immunogenic, peptide was injected into other rabbits. The antibody response against these and other peptides, as well as against solubilized human thyroid membrane (TM) and guinea pig fat cell membrane (GPF) proteins, was tested using ELISA and Western blots. The GPF and TM binding pattern of rabbits' sera was compared to that of Graves' patients' sera. We have identified an area of antigenic cross-reactivity between GPF and TM; a 63 kD protein was present in both GPF and TM, and this protein uniformly bound IgG-s of the rabbits' postimmunization sera and one of eight Graves' patient's serum. We have shown that i) a theoretically immunogenic 16 amino acid peptide was indeed highly immunogenic in rabbits, ii) antibodies binding to GPF and TM were detected after immunization, and iii) the peak of thyroid stimulating immunoglobulin activity of sera was followed by a transient elevation of serum triiodothyronine levels. Further studies investigating the immunogenic epitopes of the hTSHR as well as characterizing the 63 kD protein are indicated.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
külföldön készült közlemény
Megjelenés:Journal of Endocrinological Investigation. - 16 : 7 (1993), p. 485-493. -
További szerzők:Burch, Henry B. Lukes, Yvonne G. Carr, Frances E. Kosugi, S. Kohn, L. D. Burman, Kenneth D.
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9.

001-es BibID:BIBFORM041174
Első szerző:Nagy Endre V. (belgyógyász, endokrinológus)
Cím:Graves' IgG recognizes linear epitopes in the human thyrotropin receptor / Nagy Endre V., Burch Henry B., Mahoney Karen, Lukes Yvonne G., Morris John C., Burman Kenneth D.
Dátum:1992
ISSN:0006-291X
Megjegyzések:Twenty-nine peptides covering the full extracellular domain of the human thyrotropin receptor have been synthesized and tested for reactivity with Graves' patients' and normal sera in ELISA. Two peptides, amino acids 331-350 and the second extracellular loop of the transmembrane segment, bound IgG-s from 5 and 4 of 10 Graves' disease patients' sera, respectively. Neither of these two peptides showed enhanced binding to normal IgG. There were no apparent differences between the Graves' disease and normal group with respect to the other 27 peptides. We conclude that peptide 331-350 and the second extracellular loop carry important linear epitopes which may contribute to the disease process in selected Graves' patients.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Biochemical And Biophysical Research Communications. - 188 : 1 (1992), p. 28-33. -
További szerzők:Burch, Henry B. Mahoney, Karen Lukes, Yvonne G. Morris, John C. Burman, Kenneth D.
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10.

001-es BibID:BIBFORM041179
Első szerző:Nagy Endre V. (belgyógyász, endokrinológus)
Cím:Thyrotropin Receptor T Cell Epitopes in Autoimmune Thyroid Disease / Nagy V. E., Morris J. C., Burch H. B., Bhatia S., Salata K., Burman K. D.
Dátum:1995
ISSN:0090-1229
Megjegyzések:The human TSH receptor represents the primary target of thyroid-stimulating immunoglobulins responsible for the hyperthyroidism of Graves' disease. In the present series of investigations, the distribution of T cell epitopes has been mapped using synthetic peptides spanning the entire extracellular region of the human TSH receptor. In vitro proliferative responses of the mononuclear cells were measured using flow cytometric analysis of bromodeoxyuridine incorporation into nuclei. In 8 of 11 samples from patients with Graves' disease, at least one (and up to 9) regions of the human TSH receptor induced proliferation, with the mean stimulation index being 39.8 +/- 47.3. No single universal stimulatory peptide was identified. In contrast, stimulation was not observed in three control subjects, while one control subject showed minimal stimulation (index of 5.7) to peptides encompassing a limited area (amino acids 31-65). The immunodominant epitope of patients with recent-onset Graves' disease was localized between amino acids 271 and 365, whereas the immunodominant epitope of patients with disease duration greater than 1 year localized between amino acids 91 and 215. We conclude that the bromodeoxyuridine incorporation method is a useful and important tool for detecting antigen-induced lymphocyte proliferation. The TSH receptor-specific T cells from different Graves' disease patients recognize variable distinct sites within the extracellular region of the TSH receptor, and the immunodominant epitope apparently shifts toward the N-terminus of the receptor protein during the course of treated Graves' disease.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Clinical Immunology and Immunopathology. - 75 : 2 (1995), p. 117-124. -
További szerzők:Morris, John C. Burch, Henry B. Bhatia, Sudershan Salata, Kalman Burman, Kenneth D.
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11.

001-es BibID:BIBFORM041200
Első szerző:Nagy Endre V. (belgyógyász, endokrinológus)
Cím:Enzyme-Linked Immunosorbent Assays (ELISA) / Nagy V. E., Burman K. D.
Dátum:1993
Tárgyszavak:Orvostudományok Klinikai orvostudományok könyvfejezet
Megjelenés:Handbook of Endocrine Research Techniques / szerk. Flora de Pablo, Colin G. Scanes, Bruce D. Weintraub. - p. 55-65
További szerzők:Burman, Kenneth D.
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12.

001-es BibID:BIBFORM023976
Első szerző:Sámson L.
Cím:Addition of chlorine during water purification reduces iodine content of drinking water and contributes to iodine deficiency / Samson L., Czegeny I., Mezosi E., Erdei A., Bodor M., Cseke B., Burman K. D., Nagy E. V.
Dátum:2011
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
egyetemen (Magyarországon) készült közlemény
Megjelenés:Journal of Endocrinological Investigation 35 : 1 (2011), p. 21-24. -
További szerzők:Czégény Ildikó Mezősi Emese Erdei Annamária (1976-) (belgyógyász) Bodor Miklós (1969-) (belgyógyász, endokrinológus) Cseke Béla (1974-) (belgyógyász, endokrinológus) Burman, Kenneth D. Nagy Endre V. (1957-) (belgyógyász, endokrinológus)
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