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001-es BibID:	BIBFORM063083
Első szerző:	Lábadi Árpád
Cím:	Loss-of-Function Variants in a Hungarian Cohort Reveal Structural Insights on TSH Receptor Maturation and Signaling / Lábadi Árpád, Grassi, Elisa Stellaria, Gellén Balázs, Kleinau Gunnar, Biebermann Heike, Ruzsa Beáta, Gelmini, Giulia, Rideg Orsolya, Miseta Attila, Kovács Gábor L., Patócs Attila, Felszeghy Enikő, Nagy Endre V., Mezősi Emese, Persani, Luca
Dátum:	2015
ISSN:	0021-972X
Megjegyzések:	CONTEXT:Congenital hypothyroidism (CH) is one of the most common inborn endocrine disorders with genetic background. Despite the well-established newborn CH screening program in Hungary, no systematic examination of the underlying genetic alterations has been performed as yet.OBJECTIVE:We aimed to explore TSH receptor (TSHR) mutations in a cohort of Hungarian patients with CH.PATIENTS:Eighty-five unrelated patients with permanent primary CH, all diagnosed at newborn screening, were selected.MAIN OUTCOME MEASURES:Coding exons of the TSHR gene were sequenced and evaluated together with the thyroid-specific clinical parameters. Functional features of the novel mutations were experimentally examined, and their comparative molecular models were built.RESULTS:In four patients (one heterozygous and three compound heterozygous), seven TSHR mutations were identified. Among these, N432(1.50)D and P449(2.39)L are novel missense alterations. Importantly, the N432(1.50) residue is highly conserved among G protein-coupled receptors, and its function has not been examined yet in human glycoprotein hormone receptors. Our results indicate that the N432(1.50)D mutation disrupts important, architecture-stabilizing intramolecular interactions and ultimately leads to the complete intracellular retention of the receptor. On the other hand, P449(2.39) is located in the intracellular part of the receptor, which is important in G protein coupling. The P449(2.39)L mutation results in signaling impairment, with a more profound effect on the Gq/11 pathway.CONCLUSION:TSHR mutations are common among Hungarian patients with CH. The novel genetic alterations revealed an important structural role of the N432(1.50) and the P449(2.39) residues in receptor expression and signaling, respectively.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Congenital hypothyroidism TSH receptor newborn CH screening program
Megjelenés:	Journal Of Clinical Endocrinology & Metabolism. - 100 : 7 (2015), p. E1039-E1045. -
További szerzők:	Grassi, Elisa Stellaria Gellén Balázs Kleinau, Gunnar Biebermann, Heike Ruzsa Beáta Gelmini, Giulia Rideg Orsolya Miseta Attila Kovács Gábor L. (Szeged) Patócs Attila Felszeghy Enikő Noémi (1970-) (gyermekgyógyász) Nagy Endre V. (1957-) (belgyógyász, endokrinológus) Mezősi Emese Persani, Luca
Pályázati támogatás:	RF-2010-2309484 Egyéb KL2334/2-2 Egyéb BI 893/6-3 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM116667
035-os BibID:	(Scopus)85170843236 (WOS)001015187100005 (cikkazonosító)e220134
Első szerző:	Solymosi Tamás
Cím:	Considerable interobserver variation calls for unambiguous definitions of thyroid nodule ultrasound characteristics / Solymosi Tamas, Hegedűs Laszlo, Bonnema Steen J., Frasoldati Andrea, Jambor Laszlo, Karanyi Zsolt, Kovacs Gabor L., Papini Enrico, Rucz Karoly, Russ Gilles, Nagy Endre V.
Dátum:	2023
ISSN:	2235-0640 2235-0802
Megjegyzések:	Thyroid nodule ultrasound characteristics are used as an indication for fine-needle aspiration cytology, usually as the basis for Thyroid Imaging Reporting and Data System (TIRADS) score calculation. Few studies on interobserver variation are available, all of which are based on analysis of preselected still ultrasound images and often lack surgical confirmation.Methods: After the blinded online evaluation of video recordings of the ultrasound examinations of 47 consecutive malignant and 76 consecutive benign thyroid lesions, 7 experts from 7 thyroid centers answered 17 TIRADS-related questions. Surgical histology was the reference standard. Interobserver variations of each ultrasound characteristic were compared using Gwet's AC1 inter-rater coefficients; higher values mean better concordance, the maximum being 1.0.Results: On a scale from 0.0 to 1.0, the Gwet's AC1 values were 0.34, 0.53, 0.72, and 0.79 for the four most important features in decision-making, i.e. irregular margins, microcalcifications, echogenicity, and extrathyroidal extension, respectively. The concordance in the discrimination between mildly/moderately and very hypoechogenic nodules was 0.17. The smaller the nodule size the better the agreement in echogenicity, and the larger the nodule size the better the agreement on the presence of microcalcifications. Extrathyroidal extension was correctly identified in just 45.8% of the cases.Conclusions: Examination of video recordings, closely simulating the real-world situation, revealed substantial interobserver variation in the interpretation of each of the four most important ultrasound characteristics. In view of the importance for the management of thyroid nodules, unambiguous and widely accepted definitions of each nodule characteristic are warranted, although it remains to be investigated whether this diminishes observer variation.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk cytology thyroid cancer thyroid nodule TIRADS ultrasound
Megjelenés:	European Thyroid Journal. - 12 : 2 (2023), p. 1-11. -
További szerzők:	Hegedűs László Dávid (1992-) (PhD hallgató) Bonnema, Steen Joop Frasoldati, Andrea Jámbor László (1960-) (radiológus) Karányi Zsolt (1961-) (biostatisztikus, bioinformatikus) Kovács Gábor L. (Szeged) Papini, Enrico Rucz Károly Russ, Gilles Nagy Endre V. (1957-) (belgyógyász, endokrinológus)
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