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001-es BibID:BIBFORM041487
035-os BibID:PMID:18224288
Első szerző:Sipka Sándor (laboratóriumi szakorvos)
Cím:Adenosine inhibits the release of arachidonic acid and its metabolites (AAM) in activated human peripheral mononuclear cells / S. Sipka, I. Kovács, S. Szántó, Gy. Szegedi, L. Brugós, G. Bruckner, A. J. Szentmiklósi
Dátum:2007
ISSN:1023-3830
Megjegyzések:The effects of adenosine (Ado) and subtype-specific activators of adenosine receptors (A(1), A(2A), A(2B) and A(3)) were studied on the release of arachidonic acid (AA) and its metabolites (AAM) from human peripheral mononuclear cells (monocytes). MATERIALS AND METHOD: Adenosine and the selective agonists and antagonists of adenosine receptors were used. (3)H-AA and its metabolites released into the medium were determined by measurement of the total (3)H radioactivity released without separating the AAM. RESULTS: In the cells activated by protein kinase C specific phorbol ester (phorbol 12-myristate 13-acetate) and Ca(2+) ionophore (A23187), adenosine and two subtype-specific receptor agonists, CPA(A(1)) and CGS 21680 (A(2A)) induced concentration-dependent inhibition of the release of AAM, whereas stimulation of A(2B) or A(3) receptors was ineffective. The rank order of potency for the inhibition of AAM release was as follows: CGS 21680 = CPA > adenosine > NECA (in the presence of ZM 24185 and DPCPX as A(2A) and A(1) adenosine receptor antagonists, respectively) = IB-MECA. Adenosine inhibited the release of AAM only at and above the concentration of 100 muM, whereas the inhibitory effect of A(1) and A(2A) receptor specific agonists appeared at a concentration of 10(-7) M. CONCLUSIONS: It can be concluded that adenosine physiologically may not have a significant effect on the AAM release of circulating monocytes, but in pathological conditions, where the local Ado concentrations increases, this nucleoside, through activation of A(2A) and A(1) receptors can exert, at least in part, an antiinflammatory action by decreasing proinflammatory AAM production.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
adenosine
arachidonic acid
Adenosine/pharmacology
Arachidonic Acid/metabolism
Calcimycin/pharmacology
Calcium/metabolism
Cyclic AMP/metabolism
Humans
Ionophores/pharmacology
Monocytes/drug effects/metabolism
Protein Kinase C/metabolism
Purinergic P1 Receptor Agonists
Purinergic P1 Receptor Antagonists
Tetradecanoylphorbol Acetate/pharmacology
egyetemen (Magyarországon) készült közlemény
Megjelenés:Inflammation Research 56 : 11 (2007), p. 468-472. -
További szerzők:Kovács I. (orvos) Szántó Sándor (1968-) (belgyógyász, reumatológus) Szegedi Gyula (1936-2013) (belgyógyász, immunológus) Brugós László (1951-) (tüdőgyógyász, klinikai immunológus, allergológus) Bruckner Géza Szentmiklósi József András (1948-) (farmakológus, klinikai laboratóriumi szakorvos)
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