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001-es BibID:	BIBFORM058666
Első szerző:	Bacskai Ildikó (immunológus)
Cím:	Mesenchymal stromal cell-like cells set the balance of stimulatory and inhibitory signals in monocyte-derived dendritic cells / Ildikó Bacskai, Anett Mázló, Katalin Kis-Tóth, Attila Szabó, György Panyi, Balázs Sarkadi, Ágota Apáti, Éva Rajnavölgyi
Dátum:	2015
ISSN:	1547-3287
Megjegyzések:	The major reservoir of human multipotent mesenchymal stem/stromal cells (MSC) is the bone marrow (BM) with the capability to control hematopoietic stem cell (HSC) development. The regenerative potential of MSC is associated with enhanced endogenous repair and healing mechanisms that modulate inflammatory responses. Our previous results revealed that MSC-like (MSCl) cells derived from pluripotent human embryonic stem cells resemble BM-derived MSC in morphology, phenotype and differentiating potential. Here we investigated the effects of MSCl cells on the phenotype and functions of dendritic cells (DC). To assess how anti-viral immune responses could be regulated by intracellular pattern recognition receptors (PRR) of DC in the presence of MSCl cells we activated DC with the specific ligands of retinoic acid-inducible gene I (RIG-I) helicases and found that activated DC co-cultured with MSCl cells exhibited reduced expression of CD1a and CD83 cell surface molecules serving as phenotypic indicators of DC differentiation and activation, respectively. However, RIG-I-mediated stimulation of DC via specific ligands in the presence of MSCl cells resulted in significantly higher expression of the co-stimulatory molecules CD80 and CD86 than in the presence of BM-MSC. In line with these results the concentration of IL-6, IL-10 and CXCL8 was increased in the supernatant of the DC-MSCl co-cultures, while the secretion of TNF-?, CXCL10, IL-12 and IFN? was reduced. Furthermore, the concerted action of mechanisms involved in the regulation of DC migration resulted in the blockade of cell migration indicating altered DC functionality mediated by MSCl cell-derived signals and mechanisms resulting in a suppressive microenvironment.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban mesenchymal stromal cell dendritic cell immunsuppression RIG-like receptors matrix metalloproteinases
Megjelenés:	Stem Cells And Development. - 24 : 15 (2015), p. 1805-1816. -
További szerzők:	Türk-Mázló Anett (1989-) (molekuláris biológus) Kis-Tóth Katalin (1975-) (immunológus) Szabó Attila (1981-) (molekuláris biológus, immunológus, filozófus) Panyi György (1966-) (biofizikus) Sarkadi Balázs Apáti Ágota Rajnavölgyi Éva (1950-) (immunológus)
Pályázati támogatás:	TÁMOP 4.2.4. A/2-11-1-2012-0001 TÁMOP TÁMOP 4.2.2.A-11/1/KONV-2012-0023 TÁMOP OTKA NK 101538 OTKA
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM049592
035-os BibID:	PMID:23870824
Első szerző:	Kis-Tóth Katalin (immunológus)
Cím:	Monocyte-derived dendritic cell subpopulations use different types of matrix metalloproteinases inhibited by GM6001 / Katalin Kis-Toth, Ildiko Bacskai, Peter Gogolak, Anett Mazlo, Istvan Szatmari, Eva Rajnavolgyi
Dátum:	2013
ISSN:	0171-2985
Megjegyzések:	Matrix metalloproteinases (MMPs) are endopeptidases with the potential to cleave extracellular matrix, support tissue renewal and regulate cell migration. Functional activities of MMPs are regulated by tissue inhibitors of MMPs (TIMPs) and disruption of the MMP-TIMP balance has pathological consequences. Here we studied the expression and secretion of MMPs and TIMPs in CD1a(-) and CD1a(+) monocyte-derived dendritic cell (DC) subpopulations. Our results showed that monocytes express TIMPs but lack MMPs, whereas upon differentiation to moDCs and in response to activation signals the expression of MMPs is increased and that of TIMPs is decreased. MMP-9 is expressed dominantly in the CD1a(-) subpopulation, while MMP-12 is preferentially expressed in CD1a(+) cells. Experiments performed with the synthetic MMP inhibitor GM6001 revealed that this drug efficiently inhibits the migration of moDCs through inactivation of MMPs. We conclude that modulation of MMP activity by GM6001 emerges as a novel approach to manipulate DC migration under inflammatory conditions. (C) 2013 Elsevier GmbH. All rights reserved.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban CD1a Dendritic cell Immunotherapy Matrix metalloproteinase Tissue inhibitor of matrix Metalloproteinase
Megjelenés:	Immunobiology. - 218 : 11 (2013), p. 1361-1369. -
További szerzők:	Bacskai Ildikó (1985-) (immunológus) Gogolák Péter (1968-) (biológus, immunológus) Türk-Mázló Anett (1989-) (molekuláris biológus) Szatmári István (1971-) (biológus) Rajnavölgyi Éva (1950-) (immunológus)
Pályázati támogatás:	TÁMOP-4.2.2.A-11/1/KONV-2012-0023-"VÉD-ELEM" TÁMOP NK 101538 OTKA
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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