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001-es BibID:BIBFORM094352
035-os BibID:(cikkazonosító)5058 (scopus)85105433955 (wos)000662004700001
Első szerző:Géczi Dóra (biotechnológus)
Cím:Analysis of Circulating miRNA Profile in Plasma Samples of Glioblastoma Patients / Dóra Géczi, Bálint Nagy, Melinda Szilágyi, András Penyige, Álmos Klekner, Adrienn Jenei, József Virga, Zsuzsanna Birkó
Dátum:2021
ISSN:1661-6596 1422-0067
Megjegyzések:Background: Glioblastoma multiforme (GBM) is among the most aggressive cancers with a poor prognosis. Treatment options are limited, clinicians lack efficient prognostic and predictive markers. Circulating miRNAs?besides being important regulators of cancer development?may have potential as diagnostic biomarkers of GBM. (2) Methods: In this study, profiling of 798 human miRNAs was performed on blood plasma samples from 6 healthy individuals and 6 patients with GBM, using a NanoString nCounter Analysis System. To validate our results, five miRNAs (hsa-miR-433-3p, hsa-miR-362-3p, hsa-miR-195-5p, hsa-miR-133a-3p, and hsa-miR-29a-3p) were randomly chosen for RT-qPCR detection. (3) Results: In all, 53 miRNAs were significantly differentially expressed in plasma samples of GBM patients when data were filtered for FC 1 and FDR 0.1. Target genes of the top 39 differentially expressed miRNAs were identified, and we carried out functional annotation and pathway enrichment analysis of target genes via GO and KEGG-based tools. General and cortex-specific protein?protein interaction networks were constructed from the target genes of top miRNAs to assess their functional connections. (4) Conclusions: We demonstrated that plasma microRNA profiles are promising diagnostic and prognostic molecular biomarkers that may find an actual application in the clinical practice of GBM, although more studies are needed to validate our results.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
glioblastoma
circulating miRNA
blood plasma
network analysis
biomarker
NanoString
Megjelenés:International Journal Of Molecular Sciences. - 22 : 10 (2021), p. 1-20. -
További szerzők:Nagy Bálint (1956-) (molekuláris genetikus) Szilágyi Melinda (1984-) (biológus) Penyige András (1954-) (molekuláris genetikus) Klekner Álmos (1970-) (idegsebész) Jenei Adrienn (1978-) (biológus, kémikus) Virga József (1989-) Hádáné Birkó Zsuzsanna (1971-) (molekuláris genetikus)
Pályázati támogatás:2017-1.2.1-NKP-2017-00002
NKP
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2.

001-es BibID:BIBFORM090169
035-os BibID:(cikkazonosító)9725 (scopus)85098209070 (wos)000603503300001
Első szerző:Márton Éva (biológus)
Cím:The Cell-Free Expression of MiR200 Family Members Correlates with Estrogen Sensitivity in Human Epithelial Ovarian Cells / Éva Márton, Alexandra Varga, Lajos Széles, Lóránd Göczi, András Penyige, Bálint Nagy, Melinda Szilágyi
Dátum:2020
ISSN:1661-6596 1422-0067
Megjegyzések:Exposure to physiological estrogens or xenoestrogens (e.g., zearalenone or bisphenol A) increases the risk for cancer. However, little information is available on their significance in ovarian cancer. We present a comprehensive study on the effect of estradiol, zearalenone and bisphenol A on the phenotype, mRNA, intracellular and cell-free miRNA expression of human epithelial ovarian cell lines. Estrogens induced a comparable effect on the rate of cell proliferation and migration as well as on the expression of estrogen-responsive genes (GREB1, CA12, DEPTOR, RBBP8) in the estrogen receptor ? (ER?)-expressing PEO1 cell line, which was not observable in the absence of this receptor (in A2780 cells). The basal intracellular and cell-free expression of miR200s and miR203a was higher in PEO1, which was accompanied with low ZEB1 and high E-cadherin expression. These miRNAs showed a rapid but intermittent upregulation in response to estrogens that was diminished by an ER?-specific antagonist. The role of ER? in the regulation of the MIR200B-MIR200A-MIR429 locus was further supported by publicly available ChIP-seq data. MiRNA expression of cell lysates correlated well with cell-free miRNA expression. We conclude that cell-free miR200s might be promising biomarkers to assess estrogen sensitivity of ovarian cells.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
ovarian cancer
estrogen
zeralenone
bisphenol
estrogen receptor
cell-free miRNA
miR200
miR203a
EMT
Megjelenés:International Journal of Molecular Sciences. - 21 : 24 (2020), p. 1-19. -
További szerzők:Beke-Varga Alexandra Edit (1994-) (molekuláris biológus) Széles Lajos (1971-) (molekuláris biológus) Göczi Loránd (informatikus) Penyige András (1954-) (molekuláris genetikus) Nagy Bálint (1956-) (molekuláris genetikus) Szilágyi Melinda (1984-) (biológus)
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Intézményi repozitóriumban (DEA) tárolt változat
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3.

001-es BibID:BIBFORM080828
035-os BibID:(cikkazonosító)4533 (scopus)85072531584 (wos)000489100500212
Első szerző:Penyige András (molekuláris genetikus)
Cím:Circulating miRNA Profiling in Plasma Samples of Ovarian Cancer Patients / András Penyige, Éva Márton, Beáta Soltész, Melinda Szilágyi-Bónizs, Róbert Póka, János Lukács, Lajos Széles, Bálint Nagy
Dátum:2019
ISSN:1661-6596 1422-0067
Megjegyzések:Ovarian cancer is one of the most common cancer types in women characterized by a high mortality rate due to lack of early diagnosis. Circulating miRNAs besides being important regulators of cancer development could be potential biomarkers to aid diagnosis. We performed the circulating miRNA expression analysis in plasma samples obtained from ovarian cancer patients stratified into FIGO I, FIGO III, and FIGO IV stages and from healthy females using the NanoString quantitative assay. Forty-five miRNAs were di erentially expressed, out of these 17 miRNAs showed significantly di erent expression between controls and patients, 28 were expressed only in patients, among them 19 were expressed only in FIGO I patients. Di erentially expressed miRNAs were ranked by the network-based analysis to assess their importance. Target genes of the di erentially expressed miRNAs were identified then functional annotation of the target genes by the GO and KEGG-based enrichment analysis was carried out. A general and an ovary-specific protein?protein interaction network was constructed from target genes. Results of our network and the functional enrichment analysis suggest that besides HSP90AA1, MYC, SP1, BRCA1, RB1, CFTR, STAT3, E2F1, ERBB2, EZH2, and MET genes, additional genes which are enriched in cell cycle regulation, FOXO, TP53, PI-3AKT, AMPK, TGF , ERBB signaling pathways and in the regulation of gene expression, proliferation, cellular response to hypoxia, and negative regulation of the apoptotic process, the GO terms have central importance in ovarian cancer development. The aberrantly expressed miRNAs might be considered as potential biomarkers for the diagnosis of ovarian cancer after validation of these results in a larger cohort of ovarian cancer patients.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
ovarian cancer
circulating miRNA
blood plasma
NanoString
network analysis
bimarker
Megjelenés:International Journal Of Molecular Sciences. - 20 : 18 (2019), p. E4533. -
További szerzők:Márton Éva (1992-) (biológus) Soltész Beáta (1987-) (molekuláris biológus) Szilágyi Melinda (1984-) (biológus) Póka Róbert (1960-) (szülész-nőgyógyász, klinikai onkológus) Lukács János (1975-) (szülész-nőgyógyász, genetikus) Széles Lajos (1971-) (molekuláris biológus) Nagy Bálint (1956-) (molekuláris genetikus)
Internet cím:DOI
Intézményi repozitóriumban (DEA) tárolt változat
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4.

001-es BibID:BIBFORM099574
035-os BibID:(cikkazonosító)8 (scopus)85121366077 (wos)000751087400001
Első szerző:Soltész Beáta (molekuláris biológus)
Cím:The Role of Exosomes in Cancer Progression / Soltész Beáta, Buglyó Gergely, Németh Nikolett, Szilágyi Melinda, Pös Ondrej, Szemes Tomas, Balogh István, Nagy Bálint
Dátum:2022
ISSN:1661-6596 1422-0067
Megjegyzések:Early detection, characterization and monitoring of cancer are possible by using extracellular vesicles (EVs) isolated from non-invasively obtained liquid biopsy samples. They play a role in intercellular communication contributing to cell growth, differentiation and survival, thereby affecting the formation of tumor microenvironments and causing metastases. EVs were discovered more than seventy years ago. They have been tested recently as tools of drug delivery to treat cancer. Here we give a brief review on extracellular vesicles, exosomes, microvesicles and apoptotic bodies. Exosomes play an important role by carrying extracellular nucleic acids (DNA, RNA) in cell-to-cell communication causing tumor and metastasis development. We discuss the role of extracellular vesicles in the pathogenesis of cancer and their practical application in the early diagnosis, follow up, and next-generation treatment of cancer patients.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:International Journal Of Molecular Sciences. - 23 : 1 (2022), p. 1-19. -
További szerzők:Buglyó Gergely (1980-) (genetikus) Németh Nikolett (1995-) (biológus) Szilágyi Melinda (1984-) (biológus) Pös, Ondrej (1990-) (biológus) Szemes, Tomas (1980-) (biológus) Balogh István (1972-) (molekuláris biológus, genetikus) Nagy Bálint (1956-) (molekuláris genetikus)
Pályázati támogatás:Operational Programme Integrated Infrastructure for the project ITMS2014+
Egyéb
313011V446 European Regional Development Fund
Egyéb
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Intézményi repozitóriumban (DEA) tárolt változat
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5.

001-es BibID:bibEBI00026276
035-os BibID:(cikkazonosító)6827 (scopus)85091140313 (wos)000579918800001
Első szerző:Szilágyi Melinda (biológus)
Cím:Circulating cell-free nucleic acids : main characteristics and clinical application / Melinda Szilágyi, Ondrej Pös, Éva Márton, Gergely Buglyó, Beáta Soltész, Judit Keserű, András Penyige, Tomas Szemes, Bálint Nagy
Dátum:2020
ISSN:1661-6596 1422-0067
Megjegyzések:Liquid biopsy recently became a very promising diagnostic method that has several advantages over conventional invasive methods. Liquid biopsy may serve as a source of several important biomarkers including cell-free nucleic acids (cf-NAs). Cf-DNA is widely used in prenatal testing in order to characterize fetal genetic disorders. Analysis of cf-DNA may provide information about the mutation profile of tumor cells, while cell-free non-coding RNAs are promising biomarker candidates in the diagnosis and prognosis of cancer. Many of these markers have the potential to help clinicians in therapy selection and in the follow-up of patients. Thus, cf-NA-based diagnostics represent a new path in personalized medicine. Although several reviews are available in the field, most of them focus on a limited number of cf-NA types. In this review, we give an overview about all known cf-NAs including cf-DNA, cf-mtDNA and cell-free non-coding RNA (miRNA, lncRNA, circRNA, piRNA, YRNA, and vtRNA) by discussing their biogenesis, biological function and potential as biomarker candidates in liquid biopsy. We also outline possible future directions in the field.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
cell-free nucleic acids
nDNA
mtDNA
miRNA
lncRNA
circRNA
exosomes
biological fluids
liquid biopsy
Megjelenés:International Journal of Molecular Sciences. - 21 : 18 (2020), p. 1-20. -
További szerzők:Pös, Ondrej (1990-) (biológus) Márton Éva (1992-) (biológus) Buglyó Gergely (1980-) (genetikus) Soltész Beáta (1987-) (molekuláris biológus) Keserű Judit (1976-) (molekuláris genetikus) Penyige András (1954-) (molekuláris genetikus) Szemes, Tomas (1980-) (biológus) Nagy Bálint (1956-) (molekuláris genetikus)
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