CCL

Összesen 3 találat.
#/oldal:
Részletezés:
Rendezés:

1.

001-es BibID:BIBFORM094352
035-os BibID:(cikkazonosító)5058 (scopus)85105433955 (wos)000662004700001
Első szerző:Géczi Dóra (biotechnológus)
Cím:Analysis of Circulating miRNA Profile in Plasma Samples of Glioblastoma Patients / Dóra Géczi, Bálint Nagy, Melinda Szilágyi, András Penyige, Álmos Klekner, Adrienn Jenei, József Virga, Zsuzsanna Birkó
Dátum:2021
ISSN:1661-6596 1422-0067
Megjegyzések:Background: Glioblastoma multiforme (GBM) is among the most aggressive cancers with a poor prognosis. Treatment options are limited, clinicians lack efficient prognostic and predictive markers. Circulating miRNAs?besides being important regulators of cancer development?may have potential as diagnostic biomarkers of GBM. (2) Methods: In this study, profiling of 798 human miRNAs was performed on blood plasma samples from 6 healthy individuals and 6 patients with GBM, using a NanoString nCounter Analysis System. To validate our results, five miRNAs (hsa-miR-433-3p, hsa-miR-362-3p, hsa-miR-195-5p, hsa-miR-133a-3p, and hsa-miR-29a-3p) were randomly chosen for RT-qPCR detection. (3) Results: In all, 53 miRNAs were significantly differentially expressed in plasma samples of GBM patients when data were filtered for FC 1 and FDR 0.1. Target genes of the top 39 differentially expressed miRNAs were identified, and we carried out functional annotation and pathway enrichment analysis of target genes via GO and KEGG-based tools. General and cortex-specific protein?protein interaction networks were constructed from the target genes of top miRNAs to assess their functional connections. (4) Conclusions: We demonstrated that plasma microRNA profiles are promising diagnostic and prognostic molecular biomarkers that may find an actual application in the clinical practice of GBM, although more studies are needed to validate our results.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
glioblastoma
circulating miRNA
blood plasma
network analysis
biomarker
NanoString
Megjelenés:International Journal Of Molecular Sciences. - 22 : 10 (2021), p. 1-20. -
További szerzők:Nagy Bálint (1956-) (molekuláris genetikus) Szilágyi Melinda (1984-) (biológus) Penyige András (1954-) (molekuláris genetikus) Klekner Álmos (1970-) (idegsebész) Jenei Adrienn (1978-) (biológus, kémikus) Virga József (1989-) Hádáné Birkó Zsuzsanna (1971-) (molekuláris genetikus)
Pályázati támogatás:2017-1.2.1-NKP-2017-00002
NKP
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

2.

001-es BibID:BIBFORM108635
035-os BibID:(cikkazonosító)140 (Scopus)85148964475
Első szerző:Márton Éva (biológus)
Cím:Comparative Analysis of Transcriptomic Changes including mRNA and microRNA Expression Induced by the Xenoestrogens Zearalenone and Bisphenol A in Human Ovarian Cells / Éva Márton, Alexandra Varga, András Penyige, Zsuzsanna Birkó, István Balogh, Bálint Nagy, Melinda Szilágyi
Dátum:2023
ISSN:2072-6651
Megjegyzések:Xenoestrogens are natural or synthetic compounds that mimic the effect of endogenous estrogens and might cause cancer. We aimed to compare the global transcriptomic response to zearalenone (ZEA; mycotoxin) and bisphenol A (BPA; plastic additive) with the effect of physiological estradiol (E2) in the PEO1 human ovarian cell line by mRNA and microRNA sequencing. Estrogen exposure induced remarkable transcriptomic changes: 308, 288 and 63 genes were upregulated (log2FC > 1); 292, 260 and 45 genes were downregulated (log2FC < ?1) in response to E2 (10 nM), ZEA (10 nM) and BPA (100 nM), respectively. Furthermore, the expression of 13, 11 and 10 miRNAs changed significantly (log2FC > 1, or log2FC < ?1) after exposure to E2, ZEA and BPA, respectively. Functional enrichment analysis of the significantly differentially expressed genes and miRNAs revealed several pathways related to the regulation of cell proliferation and migration. The effect of E2 and ZEA was highly comparable: 407 genes were coregulated by these molecules. We could identify 83 genes that were regulated by all three treatments that might have a significant role in the estrogen response of ovarian cells. Furthermore, the downregulation of several miRNAs (miR-501- 5p, let-7a-2-3p, miR-26a-2-3p, miR-197-5p and miR-582-3p) was confirmed by qPCR, which might support the proliferative effect of estrogens in ovarian cells.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
xenoösztrogén
zearalenon
mikotoxin
biszfenol A
petefészekrák
transzkriptomika
mikroRNS
RNS szekvenálás
Megjelenés:Toxins. - 15 : 2 (2023), p. 1-22. -
További szerzők:Beke-Varga Alexandra Edit (1994-) (molekuláris biológus) Penyige András (1954-) (molekuláris genetikus) Hádáné Birkó Zsuzsanna (1971-) (molekuláris genetikus) Balogh István (1972-) (molekuláris biológus, genetikus) Nagy Bálint (1956-) (molekuláris genetikus) Szilágyi Melinda (1984-) (biológus)
Pályázati támogatás:FK138021
OTKA
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

3.

001-es BibID:BIBFORM077294
Első szerző:Szilágyi Melinda (biológus)
Cím:Mutation in afsR Leads to A-Factor Deficiency in Streptomyces griseus B2682 / Melinda Szilágyi, Éva Márton, Dávid Lukács, Zsuzsanna Birkó, Zoltán Kele, Sándor Biró
Dátum:2018
ISSN:1464-1801
Megjegyzések:A-factor, a γ-butyrolactone autoregulator, in Streptomyces griseus is involved in the regulation of differentiation and antibiotic production. Here we studied the S. griseus B2682-AFN (A-factor negative) bald mutant that harbors a nonsense mutation in the afsR gene encoding a pleiotropic regulator. Our aim was to prove that this mutation is the cause of the A-factor deficiency in AFN. We also studied whether AfsR regulates A-factor production by AfsA, which is supposed to be the only specific key enzyme in A-factor biosynthesis. METHODS: Wild afsR was cloned to the pHJL401 shuttle vector and was transformed to the S. griseus AFN and B2682 strains. During phenotypic characterization, sporulation, antibiotic, protease, A-factor, and AfsA protein production were studied. RESULTS: Transformation of AFN by a wild afsR restored its phenotype including sporulation, antibiotic, extracellular protease, and A-factor production. Introduction of afsR to the B2682 wild-type strain resulted in antibiotic and extracellular protease overproduction that was accompanied with an elevated A-factor level. AfsA was detected both in AFN and B2682. CONCLUSIONS: AfsR has an effect on the regulation of A-factor production in S. griseus. The presence of AfsA is not sufficient for normal A-factor production. AfsR regulates A-factor biosynthesis independently of AfsA.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Journal Of Molecular Microbiology And Biotechnology. - 28 : 5 (2018), p. 216-224. -
További szerzők:Márton Éva (1992-) (biológus) Lukács Dávid Hádáné Birkó Zsuzsanna (1971-) (molekuláris genetikus) Kele Zoltán Biró Sándor (1949-) (molekuláris genetikus)
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Rekordok letöltése1