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001-es BibID:BIBFORM033451
Első szerző:Daoud, Rosette
Cím:Ischemia induces a translocation of the splicing factor tra2-beta 1 and changes alternative splicing patterns in the brain / Daoud R., Mies G., Smialowska A., Oláh L., Hossmann K. A., Stamm S.
Dátum:2002
Megjegyzések:Alternative splice-site selection is regulated by the relative concentration of individual members of the serine-arginine family of proteins and heterogeneous nuclear ribonucleoproteins. Most of these proteins accumulate predominantly in the nucleus, and a subset of them shuttles continuously between nucleus and cytosol. We demonstrate that in primary neuronal cultures, a rise in intracellular calcium concentration induced by thapsigargin leads to a translocation of the splicing regulatory protein tra2-beta1 and a consequent change in splice-site selection. To investigate this phenomenon under physiological conditions, we used an ischemia model. Ischemia induced in the brain causes a cytoplasmic accumulation and hyperphosphorylation of tra2-beta1. In addition, several of the proteins binding to tra2-beta1, such as src associated in mitosis 68 and serine/arginine-rich proteins, accumulate in the cytosol. Concomitant with this subcellular relocalization, we observed a change in alternative splice-site usage of the ICH-1 gene. The increased usage of its alternative exons is in agreement with previous studies demonstrating its repression by a high concentration of proteins with serine/arginine-rich domains. Our findings suggest that a change in the calcium concentration associated with ischemia is part of a signaling event, which changes pre-mRNA splicing pathways by causing relocalization of proteins that regulate splice-site selection.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:The Journal of Neuroscience. - 22 : 14 (2002), p. 5889-5899. -
További szerzők:Mies, Günter Smialowska, Agata Oláh László (1967-) (neurológus) Hossmann, Konstantin-Alexander Stamm, Stefan
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2.

001-es BibID:BIBFORM033462
Első szerző:Maeda, Keiichiro
Cím:Quantitative measurement of local cerebral blood flow in the anesthetized mouse using intraperitoneal [14C]iodoantipyrine injection and final arterial heart blood sampling / Keiichiro Maeda, Gunter Mies, Laszlo Olah, Konstantin-Alexander Hossmann
Dátum:2000
Megjegyzések:Autoradiographic measurement of local cerebral blood flow (CBF) with [14C]iodoantipyrine (IAP) is limited in mice by the difficulty in cannulating vessels and the blood loss for repeated blood sampling. The authors modified and validated the method to measure local CBF with [14C]IAP in mice by combining intraperitoneal tracer application with a single blood sampling from the heart at the end of the experiment. Experiments were carried out in male SV129 mice under halothane anesthesia. After intraperitoneal administration of 15 microCi [14C]IAP, arterial blood samples were collected repeatedly and anesthetized animals were immersed in liquid nitrogen. In addition, frozen blood from the heart was sampled to obtain the final blood [14C]radioactivity. Correlation analysis between the sampling time and [14C]radioactivity of the arterial blood revealed a highly significant linear relationship (P < 0.001, r = 0.978) and a lag time of the [14C]tracer in arterial blood of 3.3 +/- 0.6 seconds. [14C]radioactivity of the final arterial blood sample (444 +/- 264 nCi/mL) was almost equal to that of the heart blood (454 +/- 242 nCi/mL), and the absolute difference in each animal was 3.3 +/- 4.2% (mean +/- SD). The convolution integrals for the CBF calculation were determined either by integrating the radioactivity of individual arterial blood samples or by assuming a linear rise from [14C]tracer lag time after intraperitoneal [14C]IAP injection to the value measured in the blood sample from the frozen heart. Regional flow values calculated by the two methods differed by less than 11% (not significant). This method allows the quantitative measurement of local CBF in anesthetized mice without any vessel catheterization and will make mutant mice a more powerful tool to elucidate the molecular mechanisms of brain injuries by combining flow studies with molecular-biological methods.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Journal of Cerebral Blood Flow and Metabolism. - 20 : 1 (2000), p. 10-14. -
További szerzők:Mies, Günter Oláh László (1967-) (neurológus) Hossmann, Konstantin-Alexander
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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3.

001-es BibID:BIBFORM033450
Első szerző:Trapp, Torsten
Cím:GTPase RhoB : an early predictor of neuronal death after transient focal ischemia in mice / Trapp T., Oláh L., Hölker I., Besselmann M., Tiesler C., Maeda K., Hossmann K. A.
Dátum:2001
ISSN:1044-7431
Megjegyzések:Applying the recently developed DNA array technique to a murine stroke model, we found that the gene coding for RhoB, a member of the family of GTPases that regulate a variety of signal transduction pathways, is upregulated in ischemia-damaged neurons. RhoB immunoreactivity precedes DNA single-strand breaks and heralds the evolving infarct, making it an early predictor of neuronal death. Expression of RhoB colocalized with drastic rearrangement of the actin cytoarchitecture indicates a role for Rho in postischemic morphological changes. Apoptosis in a murine hippocampal cell line was also associated with an early increase in RhoB protein. Activation of caspase-3, a crucial step in apoptosis, could be inhibited by cytochalasin D, a substance that counteracts the actin-modulating activity of Rho GTPases, indicating that Rho proteins may have impact on injury-initiated neuronal signal transduction. Our findings make Rho GTPases potential targets for the development of drugs aimed at limiting neuronal death following brain damage.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Molecular And Cellular Neuroscience. - 17 : 5 (2001), p. 883-894. -
További szerzők:Oláh László (1967-) (neurológus) Hölker, Irmgard Besselmann, Michael Tiesler, Christine Maeda, Keiichiro Hossmann, Konstantin-Alexander
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4.

001-es BibID:BIBFORM033453
Első szerző:van Dorsten, Ferdi A.
Cím:Dynamic changes of ADC, perfusion, and NMR relaxation parameters in transient focal ischemia of rat brain / van Dorsten F. A., Oláh L., Schwindt W., Grüne M., Uhlenküken U., Pillekamp F., Hossmann K. A., Hoehn M.
Dátum:2002
ISSN:0740-3194
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Magnetic Resonance In Medicine. - 47 : 1 (2002), p. 97-104. -
További szerzők:Oláh László (1967-) (neurológus) Schwindt, Wolfram Grüne, Matthias Uhlenküken, U. Pillekamp, F. Hossmann, Konstantin-Alexander Hoehn, Mathias
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