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001-es BibID:BIBFORM057197
Első szerző:Gyöngyösi Eszter (molekuláris biológus, mikrobiológus)
Cím:Transcriptional regulation of genes involved in keratinocyte differentiation by human papillomavirus 16 oncoproteins / Eszter Gyöngyösi, Anita Szalmás, Annamária Ferenczi, Szilárd Póliska, József Kónya, György Veress
Dátum:2015
ISSN:0304-8608
Megjegyzések:The life cycle of human papillomaviruses (HPV) is strictly linked to the differentiation of their natural host cells. The HPV E6 and E7 oncoproteins can delay the normal differentiation program of keratinocytes, however, the exact mechanisms responsible for this have not yet been identified. The goal of this study was to investigate the effects of HPV16 oncoproteins on the expression of genes involved in keratinocyte differentiation. Primary human keratinocytes transduced by LXSN (control) retroviruses or virus vectors expressing HPV16 E6, E7 or E6/E7 genes were subjected to gene expression profiling. The results of microarray analysis showed that HPV 16 E6 and E7 have the capacity to down-regulate the expression of several genes involved in keratinocyte differentiation. Quantitative real-time polymerase chain reaction (qRT-PCR) assays were performed to confirm microarray data. To investigate the effects of the HPV oncoproteins on the promoters of selected keratinocyte differentiation genes, luciferase reporter assays were performed. Our results suggest that the HPV 16 E6 and/or E7 oncogenes are able to down-regulate the expression of several genes involved in keratinocyte differentiation (such as desmocollin 1, keratin 4, S100 calcium-binding protein A8 and small proline-rich protein 1A), at least partially by down-regulating their promoter activity. This activity of the HPV oncoproteins may have a role in the productive virus life cycle, and also in virus induced carcinogenesis.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Archives of Virology. - 160 : 2 (2015), p. 389-398. -
További szerzők:Szalmás Anita (1978-) (biológus, mikrobiológus, klinikai mikrobiológus) Ferenczi Annamária (1986-) (molekuláris biológus, mikrobiológus) Póliska Szilárd (1978-) (biológus) Kónya József (1964-) (szakorvos, klinikai mikrobiológus) Veress György (1966-) (biológus, mikrobiológus)
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