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001-es BibID:	BIBFORM048835
Első szerző:	Dózsa Anikó (Ph.D hallgató, orvos)
Cím:	PPARγ-Mediated and Arachidonic Acid-Dependent Signaling is Involved in Differentiation and Lipid Production of Human Sebocytes / Aniko Dozsa, Balazs Dezso, Balazs I. Toth, Attila Bacsi, Szilard Poliska, Emanuela Camera, Mauro Picardo, Christos C. Zouboulis, Tamás Bíró, Gerd Schmitz, Gerhard Liebisch, Ralph Rühl, Eva Remenyik, Laszlo Nagy
Dátum:	2014
ISSN:	0022-202X
Megjegyzések:	The transcriptional basis of sebocyte differentiation and lipid production is mostly unclear. Peroxisome proliferator-activated receptor gamma (PPAR?), a lipid activated transcription factor, has been implicated in differentiation and lipid metabolism of various cell types. Here, we show that PPAR? is differentially expressed in normal and pathological human sebocytes, and appears to have roles in their differentiation and lipid production. We used laser microdissected normal and pathologic human sebaceous glands (SG) and SZ95 cells (immortalized sebocyte cell line) analyzed by RT-qPCR and immunohistochemistry. Lipids were analyzed by quantitative fluorimetry and mass spectrometry based approaches. We have observed that PPAR? and its target genes, ADRP and PGAR, are expressed in sebocytes and show association with their level of differentiation. Also, PPAR? is present in normal and hyperplastic SG, whereas its expression levels are decreased in SG adenoma and SG carcinoma cells, reflecting a maturation-linked expression pattern. Furthermore, in SZ95 sebocytes, naturally occurring lipids including arachidonic acid and arachidonic acid keto-metabolites (eg 5-KETE, 12-KETE) appear to regulate PPAR? signaling pathways, which in turn modulate phospholipid biosynthesis and induce neutral lipid synthesis. Collectively, our findings highlight the importance of endogenous ligand-activated PPAR? signaling in human sebocyte biology and suggest that PPAR? might be a promising candidate for the clinical management of SG disorders.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Calcium (Ca2+) signalling Ion channel Membrane transport Molecular mechanisms of disease Skin TRP channels
Megjelenés:	Journal of Investigative Dermatology. - 134 : 4 (2014), p. 910-920. -
További szerzők:	Dezső Balázs (1951-) (pathológus) Tóth István Balázs (1978-) (élettanász) Bácsi Attila (1967-) (immunológus) Póliska Szilárd (1978-) (biológus) Camera, Emanuela Picardo, Mauro Zouboulis, Christos C. (1960-) (bőrgyógyász) Bíró Tamás (1968-) (élettanász) Schmitz, Gerd Liebisch, Gerhard Rühl, Ralph (1969-) (vegyész) Remenyik Éva (1956-) (bőrgyógyász) Nagy László (1966-) (molekuláris sejtbiológus, biokémikus)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM113710
035-os BibID:	(cikkazonosító)3315 (WoS)001046283500001 (Scopus)85167680789
Első szerző:	Kovács Dóra (Biológus)
Cím:	Linoleic Acid Induced Changes in SZ95 Sebocytes-Comparison with Palmitic Acid and Arachidonic Acid / Dóra Kovács, Emanuela Camera, Szilárd Póliska, Alessia Cavallo, Miriam Maiellaro, Katalin Dull, Florian Gruber, Christos C. Zouboulis, Andrea Szegedi, Dániel Törőcsik
Dátum:	2023
ISSN:	2072-6643
Megjegyzések:	Linoleic acid (LA) is an essential omega-6 polyunsaturated fatty acid (PUFA) derived from the diet. Sebocytes, whose primary role is to moisturise the skin, process free fatty acids (FFAs) to produce the lipid-rich sebum. Importantly, like other sebum components such as palmitic acid (PA), LA and its derivative arachidonic acid (AA) are known to modulate sebocyte functions. Given the different roles of PA, LA and AA in skin biology, the aim of this study was to assess the specificity of sebocytes for LA and to dissect the different roles of LA and AA in regulating sebocyte functions. Using RNA sequencing, we confirmed that gene expression changes in LA-treated sebocytes were largely distinct from those induced by PA. LA, but not AA, regulated the expression of genes related to cholesterol biosynthesis, androgen and nuclear receptor signalling, keratinisation, lipid homeostasis and differentiation. In contrast, a set of mostly down-regulated genes involved in lipid metabolism and immune functions overlapped in LA- and AA-treated sebocytes. Lipidomic analyses revealed that the changes in the lipid profile of LA-treated sebocytes were more pronounced than those of AA-treated sebocytes, suggesting that LA may serve not only as a precursor of AA but also as a potent regulator of sebaceous lipogenesis, which may not only influence the gene expression profile but also have further specific biological relevance. In conclusion, we have shown that sebocytes are able to respond selectively to different lipid stimuli and that LA-induced effects can be both AA-dependent and independent. Our findings allow for the consideration of LA application in the therapy of sebaceous gland-associated inflammatory skin diseases such as acne, where lipid modulation and selective targeting of AA metabolism are potential treatment options.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Nutrients. - 15 : 15 (2023), p. 1-20. -
További szerzők:	Camera, Emanuela Póliska Szilárd (1978-) (biológus) Cavallo, Alessia Maiellaro, Miriam Dull Katalin (1983-) (molekuláris biológus, genetikus) Gruber, Florian Zouboulis, Christos C. (1960-) (bőrgyógyász) Szegedi Andrea (1964-) (bőrgyógyász) Töröcsik Dániel (1979-) (bőrgyógyász)
Pályázati támogatás:	FK-132296 OTKA ANN 139589 OTKA K-128250 OTKA Italian Ministry of Health Egyéb FWF Austrian Science Fund; I 5627-B Egyéb
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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