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001-es BibID:BIBFORM057197
Első szerző:Gyöngyösi Eszter (molekuláris biológus, mikrobiológus)
Cím:Transcriptional regulation of genes involved in keratinocyte differentiation by human papillomavirus 16 oncoproteins / Eszter Gyöngyösi, Anita Szalmás, Annamária Ferenczi, Szilárd Póliska, József Kónya, György Veress
Dátum:2015
ISSN:0304-8608
Megjegyzések:The life cycle of human papillomaviruses (HPV) is strictly linked to the differentiation of their natural host cells. The HPV E6 and E7 oncoproteins can delay the normal differentiation program of keratinocytes, however, the exact mechanisms responsible for this have not yet been identified. The goal of this study was to investigate the effects of HPV16 oncoproteins on the expression of genes involved in keratinocyte differentiation. Primary human keratinocytes transduced by LXSN (control) retroviruses or virus vectors expressing HPV16 E6, E7 or E6/E7 genes were subjected to gene expression profiling. The results of microarray analysis showed that HPV 16 E6 and E7 have the capacity to down-regulate the expression of several genes involved in keratinocyte differentiation. Quantitative real-time polymerase chain reaction (qRT-PCR) assays were performed to confirm microarray data. To investigate the effects of the HPV oncoproteins on the promoters of selected keratinocyte differentiation genes, luciferase reporter assays were performed. Our results suggest that the HPV 16 E6 and/or E7 oncogenes are able to down-regulate the expression of several genes involved in keratinocyte differentiation (such as desmocollin 1, keratin 4, S100 calcium-binding protein A8 and small proline-rich protein 1A), at least partially by down-regulating their promoter activity. This activity of the HPV oncoproteins may have a role in the productive virus life cycle, and also in virus induced carcinogenesis.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Archives of Virology. - 160 : 2 (2015), p. 389-398. -
További szerzők:Szalmás Anita (1978-) (biológus, mikrobiológus, klinikai mikrobiológus) Ferenczi Annamária (1986-) (molekuláris biológus, mikrobiológus) Póliska Szilárd (1978-) (biológus) Kónya József (1964-) (szakorvos, klinikai mikrobiológus) Veress György (1966-) (biológus, mikrobiológus)
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001-es BibID:BIBFORM094421
Első szerző:László Brigitta (molekuláris biológus, mikrobiológus)
Cím:Coordinated Action of Human Papillomavirus Type 16 E6 and E7 Oncoproteins on Competitive Endogenous RNA (ceRNA) network members in Primary Human Keratinocytes / Brigitta László, László Antal, Eszter Gyöngyösi, Anita Szalmás, Szilárd Póliska, György Veress, József Kónya
Dátum:2021
ISSN:1471-2407
Megjegyzések:Background: miRNAs and lncRNAs can regulate cellular biological processes both under physiological and pathological conditions including tumour initiation and progression. Interactions between differentially expressed diverse RNA species, as a part of a complex intracellular regulatory network (ceRNA network), may contribute also to the pathogenesis of HPV-associated cancer. The purpose of this study was to investigate the global expression changes of miRNAs, lncRNAs and mRNAs driven by the E6 and E7 oncoproteins of HPV16, and construct a corresponding ceRNA regulatory network of coding and non-coding genes to suggest a regulatory network associated with high-risk HPV16 infections. Furthermore, additional GO and KEGG analyses were performed to understand the consequences of mRNA expression alterations on biological processes. Methods: Small and large RNA deep sequencing were performed to detect expression changes of miRNAs, lncRNAs and mRNAs in primary human keratinocytes expressing HPV16 E6, E7 or both oncoproteins. The relationships between lncRNAs, miRNAs and mRNAs were predicted by using StarBase v2.0, DianaTools-LncBase v.2 and miRTarBase. The lncRNA-miRNA-mRNA regulatory network was visualized with Cytoscape v3.4.0. GO and KEEG pathway enrichment analysis was performed using DAVID v6.8. Results: We revealed that 85 miRNAs in 21 genomic clusters and 41 lncRNAs were abnormally expressed in HPV E6/E7 expressing cells compared with controls. We constructed a ceRNA network with members of 15 lncRNAs ? 43 miRNAs ? 358 mRNAs with significantly altered expressions. GO and KEGG functional enrichment analyses identified numerous cancer related genes, furthermore we recognized common miRNAs as key regulatory elements in biological pathways associated with tumorigenesis driven by HPV16. Conclusions: The multiple molecular changes driven by E6 and E7 oncoproteins resulting in the malignant transformation of HPV16 host cells occur, at least in part, due to the abnormal alteration in expression and function of non-coding RNA molecules through their intracellular competing network.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Bmc Cancer. - 21 : 1 (2021), p. 673. -
További szerzők:Antal László (1984-) (hidrobiológus, biológus-ökológus) Gyöngyösi Eszter (1983-) (molekuláris biológus, mikrobiológus) Szalmás Anita (1978-) (biológus, mikrobiológus, klinikai mikrobiológus) Póliska Szilárd (1978-) (biológus) Veress György (1966-) (biológus, mikrobiológus) Kónya József (1964-) (szakorvos, klinikai mikrobiológus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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