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1.

001-es BibID:BIBFORM002004
Első szerző:Csánky Eszter (tüdőgyógyász, klinikai immunológus, allergológus)
Cím:Monoclonal antibody proteomics : discovery and prevalidation of chronic obstructive pulmonary disease biomarkers in a single step / Eszter Csanky, Petra Olivova, Eva Rajnavolgyi, William Hempel, Nadege Tardieu, Elesne Toth Katalin, Anne Jullien, Carole Malderez-Bloes, Mariana Kuras, Manuel X. Duval, Laszlo Nagy, Beata Scholtz, William Hancock, Barry Karger, András Guttman, Laszlo Takacs
Dátum:2007
Megjegyzések:We define mAb proteomics as the global generation of disease specific antibodies that permit mass screening of biomarkers. An integrated, high-throughput, disease-specific mAb-based biomarker discovery platform has been developed. The approach readily provided new biomarker leads with the focus on large-scale discovery and production of mAb-based, disease-specific clinical assay candidates. The outcome of the biomarker discovery process was a highly specific and sensitive assay, applicable for testing of clinical validation paradigms, like response to treatment or correlation with other clinical parameters. In contrast to MS-based or systems biology-based strategies, our process produced prevalidated clinical assays as the outcome of the discovery process. By re-engineering the biomarker discovery paradigm, the encouraging results presented in this paper clearly demonstrate the efficiency of the mAb proteomics approach, and set the grounds for the next steps of studies, namely, the hunt for candidate biomarkers that respond to drug treatment.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
antibody
biomarker discovery
proteomics
Megjelenés:Electrophoresis 28 : 23 (2007), p. 4401-4406. -
További szerzők:Olivova, Petra Rajnavölgyi Éva (1950-) (immunológus) Hempel, William Tardieu, Nadège Élesné Tóth Katalin Jullien, Anne Malderez-Bloes, Carole Kuras, Mariana Duval, Manuel X. Nagy László Scholtz Beáta (1967-) (biokémikus, molekuláris biológus) Hancock, William Karger, Barry Guttman András (1954-) (vegyészmérnök) Takács László (1955-)
Internet cím:elektronikus változat
DOI
elektronikus változat
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2.

001-es BibID:BIBFORM023718
035-os BibID:(cikkazonosító)M111.010298 (WoS)000298290300017 (Scopus)83055168546
Első szerző:Guergova-Kuras, Mariana
Cím:Discovery of lung cancer biomarkers by profiling the plasma proteome with monoclonal antibody libraries / Mariana Guergova-Kuras, István Kurucz, William Hempel, Nadege Tardieu, János Kádas, Carole Malderez-Bloes, Anne Jullien, Yann Kieffer, Marina Hincapie, András Guttman, Eszter Csánky, Balázs Dezső, Barry L. Karger, László Takács
Dátum:2011
ISSN:1535-9476 1535-9484
Megjegyzések:A challenge in the treatment of lung cancer is the lack of early diagnostics. Here, we describe the application of monoclonal antibody (mAb) proteomics for discovery of a panel of biomarkers for early detection (stage I) of non small cell lung cancer (NSCLC). We produced large monoclonal antibody libraries directed against the natural form of protein antigens present in the plasma of NSCLC patients. Plasma biomarkers associated with the presence of lung cancer were detected via high throughput ELISA. Differential profiling of plasma proteomes of four clinical cohorts, totalling 301 patients with lung cancer and 235 healthy controls, identified 13 lung cancer associated (p<0.05) monoclonal antibodies. The mAbs recognize five different cognate proteins identified using immunoprecipitation followed by mass spectrometry. Four of the five antigens were present in non-small cell lung cancer cells in-situ. The approach is capable of generating independent antibodies against different epitopes of the same proteins, allowing fast translation to multiplexed sandwich assays. Based on these results, we have verified in two independent clinical collections a panel of five biomarkers for classifying patient disease status with a diagnostics performance of 77% sensitivity and 87% specificity. Combining CYFRA, an established cancer marker, with the panel resulted in a performance of 83 % sensitivity at 95 % specificity for stage I NSCLC.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Molecular and Cellular Proteomics. - 10 : 12 (2011), p. 1-14. -
További szerzők:Kurucz István Hempel, William Tardieu, Nadège Kádas János (1976-) (molekuláris biológus, biokémikus, kertészmérnök) Malderez-Bloes, Carole Jullien, Anne Kieffer, Yann Hincapie, Marina Guttman András (1954-) (vegyészmérnök) Csánky Eszter (1959-) (tüdőgyógyász, klinikai immunológus, allergológus) Karger, Barry Takács László (1955-) Dezső Balázs (1951-) (pathológus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
DOI
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3.

001-es BibID:BIBFORM069407
Első szerző:Guttman András (vegyészmérnök)
Cím:Biomarker Discovery by Monoclonal Antibody Proteomics / A. Guttman, B. L. Karger, W. S. Hancock, L. Takacs
Dátum:2006
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Molecular & Cellular Proteomics. - 5 : 10 (2006), 86. p. -
További szerzők:Karger, Barry Hancock, William Takács László (1955-)
Internet cím:Intézményi repozitóriumban tárolt változat
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4.

001-es BibID:BIBFORM069405
Első szerző:Hempel, William
Cím:Biomarker Discovery and Validation in One Step : combination of High Throughput Monoclonal Antibody (MAB) and Mass Spectrometry Technologies / W. Hempel, P. Olivova, E. Rajnavolgyi, B. Karger, W. Hancock, L. Takacs
Dátum:2005
ISSN:1535-9476
Tárgyszavak:Orvostudományok Elméleti orvostudományok idézhető absztrakt
Megjelenés:Molecular & Cellular Proteomics. - 4 : 8 (2005), 366. p. -
További szerzők:Olivova, Petra Rajnavölgyi Éva (1950-) (immunológus) Karger, Barry Hancock, William Takács László (1955-)
Internet cím:Intézményi repozitóriumban tárolt változat
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5.

001-es BibID:BIBFORM082285
Első szerző:Takács László
Cím:Non-small cell lung cancer biomarkers : discovery using mab proteomics / L. Takacs, M. Guergova-Kuras, I. Kurucz, N. Tardieu, J. Kadas, C. Malderez-Bloes, A. Jullien, Y. Kieffer, B. Dezso, B. Karger
Dátum:2011
ISSN:0732-183X 1527-7755
Megjegyzések:Background: A challenge in the treatment of lung cancer is the lack of early, pre-symptomatic diagnosis. Here, we describe the application of monoclonal antibody proteomics approach to profile the natural plasma proteome for the discovery of lung cancer biomarkers. Methods: We produced large monoclonal antibody (mAb) libraries directed against the natural form of complex mixtures of protein antigens present in the plasma of non-small cell lung cancer (NSCLC) patients. Lung cancer specific biomarkers in the plasma were detected via high throughput ELISA with mAbs. Antigen identification and specificity of the selected antibodies was determined using immunoprecipitation followed by mass spectrometric analysis. The presence of the biomarkers in non-small cell lung cancer tissues was validated by immunohistochemistry. Results: Differential profiling of plasma proteomes of four clinical collections, totalling 301 patients with lung cancer and 235 healthy controls, identified twenty four monoclonal antibodies recognizing protein biomarkers specific for NSCLC. The majority of the selected mAbs detect antigens present in non-small cell lung cancer cells in-situ. Our study confirms previously reported circumstantial evidences for the association with lung cancer for four of the identified biomarkers and provides an independent validation in larger multi-centric clinical collection for the association of their plasma concentrations to the presence of lung cancer. Multivariate analysis of the biomarker results generated with one of these collections (214 NSCLC cases of which 128 in stage I and 169 healthy controls) yielded a five biomarkers classifier that could discriminate NSCLC cases from healthy controls with 77% sensitivity and 87% specificity. The performance of the classifier was validated in an independent collection and combination with well known a NSCLC biomarkers showed additive effect and combined performance of 84 % sensitivity at 95 % specificity. Conclusions: Using the mAb proteomics approach we have identified a panel of monoclonal antibodies associated with specific protein biomarkers that could be readily transferred to a simple screening test for the early detection of non-small cell lung cancer.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idézhető absztrakt
folyóiratcikk
Megjelenés:Journal of Clinical Oncology. - 29 : 15 suppl. (2011), p. 10561-10561. -
További szerzők:Guergova-Kuras, Mariana Kurucz István Tardieu, Nadège Kádas János (1976-) (molekuláris biológus, biokémikus, kertészmérnök) Malderez-Bloes, Carole Jullien, Anne Kieffer, Yann Dezső Balázs (1951-) (pathológus) Karger, Barry
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
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6.

001-es BibID:BIBFORM069419
Első szerző:Takács László
Cím:Monoclonal antibody based biomarker discovery and development platform / Laszlo Takacs, Andras Guttman, William S. Hancock, Barry L. Karger, Manuel Duval, Patrick Berna
Dátum:2013
Megjelenés:USA, 2013
Terjedelem:19 p.
Tárgyszavak:Orvostudományok Elméleti orvostudományok szabadalom
További szerzők:Guttman András (1954-) (vegyészmérnök) Hancock, William Karger, Barry Duval, Manuel X. Berna, Patrick
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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7.

001-es BibID:BIBFORM069265
Első szerző:Wang, Dongdong
Cím:Antigen Identification and Characterization of Lung Cancer Specific Monoclonal Antibodies Produced by mAb Proteomics / Wang Dongdong, Hincapie Marina, Guergova-Kuras Mariana, Kadas Janos, Takacs Laszlo, Karger Barry L.
Dátum:2010
ISSN:1535-3893
Megjegyzések:A mass spectrometric (MS)-based strategy for antigen (Ag) identification and characterization of globallyproduced monoclonal antibodies (mAbs) is described. Mice were immunized with a mixture of nativeglycoproteins, isolated from the pooled plasma of patients with nonsmall cell lung cancer (NSCLC), togenerate a library of IgG-secreting hybridomas. Prior to immunization, the pooled NSCLC plasma wassubjected to 3-sequential steps of affinity fractionation, including high abundant plasma proteindepletion, glycoprotein enrichment, and polyclonal antibody affinity chromatography normalization.In this paper, to demonstrate the high quality of the globally produced mAbs, we selected 3 mAbs ofhigh differentiating power against a matched, pooled normal plasma sample. After production of largequantities of the mAbs from ascites fluids, Ag identification was achieved by immunoaffinity purification,SDS-PAGE, Western blotting, and MS analysis of in-gel digest products. One antigen was found to becomplement factor H, and the other two were mapped to different subunits of haptoglobin (Hpt). The2 Hpt mAbs were characterized in detail to assess the quality of the mAbs produced by the globalstrategy. The affinity of one of the mAbs to the Hpt native tetramer form was found to have a KD ofroughly 10-9 M and to be 2 orders of magnitude lower than the reduced form, demonstrating thepower of the mAb proteomics technology in generating mAbs to the natural form of the proteinsin blood. The binding of this mAb to the -chain of haptoglobin was also dependent on glycosylationon this chain. The characterization of mAbs in this work reveals that the global mAb proteomicsprocess can generate high-quality lung cancer specific mAbs capable of recognizing proteins intheir native state.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
monoclonal antibody
mAb proteomics
antibody characterization
glycoprotein
haptoglobin
conformational epitope
lung cancer
Megjelenés:Journal Of Proteome Research. - 9 : 4 (2010), p. 1834-1842. -
További szerzők:Hincapie, Marina Guergova-Kuras, Mariana Kádas János (1976-) (molekuláris biológus, biokémikus, kertészmérnök) Takács László (1955-) Karger, Barry
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
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