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001-es BibID:BIBFORM069267
Első szerző:Melendez, Alirio J.
Cím:Human sphingosine kinase: molecular cloning, functional characterization and tissue distribution / Alirio J. Melendez, Estelle Carlos-Dias, Mark Gosink, Janet M. Allen, Laszlo Takacs
Dátum:2000
ISSN:0378-1119
Megjegyzések:Sphingosine-1-phosphate (SPP), the product of sphingosine kinase, is an important signaling molecule with intra- andextracellular functions. The cDNA for the mouse sphingosine kinase has recently been reported. In this paper we describe thecloning, expression and characterization of the human sphingosine kinase (huSPHK1). Sequence analysis comparison revealedthat this kinase is evolutionarily very conserved, having a high degree of homology with the murine enzyme, and presentingseveral conserved regions with bacteria, yeast, plant, and mammalian proteins. Expressed huSPHK1 cDNA specifically phosphorylatesD-erythro-sphingosine and, to a lesser extent, D,L-erythro-dihydrosphingosine, and not at all the ♭threo' isoforms ofdihydrosphingosine; hydroxy-ceramide or non-hydroxy-ceramide; diacylglycerol (DAG); phosphatidylinositol (PI ); phosphatidylinositol-4-phosphate (PIP); or phosphatidylinositol-4,5-bisphosphate (PIP2). huSPHK1 shows typical Michaelis?Menten kinetics(Vmax=56 mM and Km=5 mM). The kinase is inhibited by D,L-threo-dihydrosphingosine (Ki=3 mM), and by N,N-dimethylsphingosine(Ki=5 mM). Northern blots indicate highest expression in adult lung and spleen, followed by peripheral bloodleukocyte, thymus and kidney, respectively. It is also expressed in brain and heart. In addition, database searches with thestSG2854 sequence indicate that huSPHK1 is also expressed in endothelial cells, retinal pigment epithelium, and senescentfibroblasts. ? 2000 Elsevier Science B.V. All rights reserved.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Cloning
Human
Sphingosine kinase
Sphingosine-1-phosphate
Megjelenés:Gene. - 251 : 1 (2000), p. 19-26. -
További szerzők:Carlos-Dias, Estelle Gosink, Mark Allen, Janet M. Takács László (1955-)
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