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001-es BibID:	BIBFORM069318
Első szerző:	Smith, Mark R.
Cím:	Direct evidence for an intracellular role for tumor necrosis factor-alpha 1 : microinjection of tumor necrosis factor kills target cells / Mark R. Smith, William E. Munger, Hsiang-Fu Kung, Laszlo Takacs, Scott K. Durum
Dátum:	1990
Megjegyzések:	TNF-alpha is a small peptide cytokine produced primarily by activated macrophages. One of the many biologic activities of TNF is the killing of diverse types of tumor cells. We considered the possibility that killing was mediated by TNF itself at an intracellular site, subsequent to receptor-mediated endocytosis. To test this hypothesis, we microinjected TNF into various murine normal cells and cell lines, some of which were killed by TNF given by the usual extracellular route, and others that were not. Cytotoxic effects of microinjected TNF were observed in several cell types 2 to 4 h after injection. L929 fibroblasts were killed by either extracellular or intracellular TNF. A TNF-resistant subline of L929 was insensitive to either extracellular or intracellular TNF. L6 fibroblasts were found to be resistant to high doses of TNF given either extracellularly or microinjected. Normal macrophages and the J774 macrophage-like cell line were not killed by extracellular TNF, but were rapidly killed by microinjected TNF. Thus, TNF, an extracellular peptide ligand, has an intracellular activity, suggesting that internalization of this ligand may have important intracellular biochemical roles.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	The Journal of Immunology 144 : 1 (1990), p. 162-169. -
További szerzők:	Munger, William E. Kung, Hsiang-Fu Takács László (1955-) Durum, Scott K.
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM069320
Első szerző:	Takács László
Cím:	Detection of IL-1 alpha and IL-1 beta gene expression by in situ hybridization : tissue localization of IL-1 mRNA in the normal C57BL/6 mouse / László Takács, Elizabeth J. Kovacs, Mark R. Smith, Howard A. Young, Scott K. Durum
Dátum:	1988
Megjegyzések:	IL-1 is a cytokine with a wide variety of effects on cells involved in inflammatory and immune responses, hemopoiesis, and bone formation. Many cell types have been shown to produce IL-1 in vitro; however, very little is known about the source and role of IL-1 in vivo. By using in situ hybridization, we examined the tissue distribution of cells containing IL-1 mRNA in normal C57BL/6 mice. The results show that many organs contain IL-1 mRNA-positive cells, but the highest frequency was found in lymphoid organs. The distribution and localization of these cells suggest that many of the IL-1 mRNA-producing cells are tissue macrophages. Organs exposed to environmental Ag and microbial products (lymph nodes, liver, intestine, lung, and uterus) had high frequencies of IL-1 mRNA-producing cells, suggesting that IL-1 is produced in local inflammatory or immune responses in vivo. The production of IL-1 mRNA in the thymus and in the bone marrow suggests that IL-1 is available to play physiologic roles in T cell differentiation and in hemopoiesis.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	The Journal of Immunology 141 : 9 (1988), p. 3081-3095. -
További szerzők:	Kovács Erzsébet J. Smith, Mark R. Young, Howard A. Durum, Scott K.
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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