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001-es BibID:	BIBFORM069331
Első szerző:	Szél Ágoston
Cím:	Heterogeneity of chicken photoreceptors as defined by hybridoma supernatants : an immunocytochemical study / Á. Szél, L. Takács, É. Monostori, I. Vigh-Teichmann, P. Röhlich
Dátum:	1985
ISSN:	0302-766X
Megjegyzések:	Immune cells producing antibodies to chicken photoreceptor membranes were fused with myeloma cells and supernatants of the resulting hybridoma cells were used to test various types of photoreceptor cells in the chicken retina by means of immunocytochemistry. A polyclonal antibody raised against the protein component of bovine rhodopsin was also used. Outer segments of various photoreceptor cells were labelled by the following antibodies: rods were positive with the anti-rhodopsin antibody, both members of the double cones were stained by supernatant A1, while one type of single cones (designated as type A) was specifically labelled by supernatants A5, B3 and D6. The other type of single cones (type B) reacted with anti-rhodopsin and supernatant A1. The results indicate that there are distinct differences in the molecular structure of various photoreceptor outer segments.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Cell and Tissue Research 240 : 3 (1985), p. 735-741. -
További szerzők:	Takács László (1955-) Monostori Éva Vigh-Teichmann I. Röhlich Pál
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM069325
Első szerző:	Szél Ágoston
Cím:	Monoclonal antibody-recognizing cone visual pigment / Á. Szél, L. Takács, É. Monostori, T. Diamantstein, I. Vigh-Teichmann, P. Röhlich
Dátum:	1986
ISSN:	0014-4835
Megjegyzések:	Monoclonal antibodies were raised to a crude photoreceptor-membrane suspension from chicken retinas. Clones producing antibodies against cone outer segments were selected by screening with immunocytochemistry on semithin sections of the retina. One monoclonal antibody, called COS-1, specifically labelled outer segments of double cones and one type of single cones; outer segments of rods and several single cones were not stained. On immunoblots of retinal photoreceptor membranes, this antibody recognized a protein with an apparent molecular mass of 33,000. The visual pigment character of the 33,000 protein was indirectly established by another monoclonal antibody, OS-2, which labelled all outer segments on semithin sections and four bands (33,000-, 36,000-, 38,000-40,000- and a composite band between 66,000-72,000 MW) on immunoblots. Of these, the 36,000- and the 72,000 MW protein bands were identified with an anti-rhodopsin polyclonal antibody as rhodopsin monomer and dimer. Monoclonal antibody OS-2 is assumed therefore to represent an antibody against a common epitope of all visual pigments of the chicken. The monoclonal antibody COS-1 was found to bind to certain cone outer segments of many other vertebrate species as well.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Experimental Eye Research 43 : 6 (1986), p. 871-883. -
További szerzők:	Takács László (1955-) Monostori Éva Diamantstein Tibor Vigh-Teichmann I. Röhlich Pál
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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