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1.

001-es BibID:BIBFORM021613
Első szerző:Csoma Eszter (molekuláris biológus, mikrobiológus)
Cím:Dominance of variant A in human Herpesvirus 6 viraemia after renal transplantation / Csoma E., Mészáros B., Gáll T., Asztalos L., Kónya J., Gergely L.
Dátum:2011
Megjegyzések:Human herpesvirus 6 (HHV-6), mostly variant B reactivation in renal transplant patients has been published by other authors, but the pathogenetic role of HHV-6 variant A has not been clarified. Our aims were to examine the prevalence of HHV-6, to determine the variants, and to investigate the interaction between HHV-6 viraemia, human cytomegalovirus (HCMV) infection and clinical symptoms. METHODS: Variant-specific HHV-6 nested PCR and quantitative real-time PCR were used to examine blood samples from renal transplant patients and healthy blood donors for the presence and load of HHV-6 DNA and to determine the variants. Active HHV-6 infection was proved by RT-PCR, and active HCMV infection was diagnosed by pp65 antigenaemia test. RESULTS: HHV-6 viraemia was significantly more frequent in renal transplant patients compared to healthy blood donors (9/200 vs. 0/200; p = 0.004), while prevalence of HHV-6 latency was not significantly different (13/200 vs. 19/200; p > 0.05). Dominance of variant A was revealed in viraemias (8/9), and the frequency of HHV-6A was significantly higher in active infections compared with latency in renal transplant patients (8/9 vs. 2/13; p = 0.0015). Latency was established predominantly by HHV-6B both in renal transplant patients and in healthy blood donors (11/13 and 18/19). There was no statistical significant difference in occurrence of HCMV and HHV-6 viraemia in renal transplant patients (7/200 vs. 9/200). Statistical analysis did not reveal interaction between HHV-6 viraemia and clinical symptoms in our study. CONCLUSIONS: Contrary to previous publications HHV-6A viraemia was found to be predominant in renal transplant patients. Frequency of variant A was significantly higher in cases of active infection then in latency.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Virology Journal [electronic resource]. - 8 (2011), p. 403. -
További szerzők:Mészáros Beáta (1985-) (molekuláris biológus, mikrobiológus) Gáll Tamás (1982-) (molekuláris biológus, mikrobiológus) Asztalos László (1951-) (sebész) Kónya József (1964-) (szakorvos, klinikai mikrobiológus) Gergely Lajos (1940-) (szakorvos, klinikai mikrobiológus)
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2.

001-es BibID:BIBFORM032221
Első szerző:Fehér Enikő (molekuláris biológus, mikrobiológus)
Cím:Investigation of the occurrence of torque tenovirus in malignant and potentially malignant disorders associated with human papillomavirus / Fehér Enikő, Gáll Tamás, Murvai Melinda, Kis Andrea, Boda Róbert, Sápy Tamás, Tar Ildikó, Gergely Lajos, Szarka Krisztina
Dátum:2009
ISSN:0146-6615
Megjegyzések:In a previous pilot study, a significantly poorer outcome of laryngeal cancer was found in patients co-infected with human papillomavirus (HPV) and genogroup 1 torque tenovirus (TTV). The present study aimed to collect data on the overall prevalence of TTVs on the prevalence of genogroup 1 TTV in two other malignancies associated with HPV,oralsquamouscell cancerandcervical cancer, and in oral and cervical premalignant lesions (oral lichen planus, oral leukoplakia, cervical atypia). Oral samples from all patients were accompanied with asamplefromthehealthymucosa.Theoverall prevalence of TTV was significantly higher both in oral squamous cell cancer and cervical cancer compared with other patient groups or with the respective controls.The prevalenceofgenogroup 1 TTV was significantly higher in lesions of oral squamous cell cancer and oral lichen planus, but not in lesions of oral leukoplakia (24.6%, 10.1%, and 4.5%, respectively), compared with the prevalence in the oral cavity of controls (1.4%). Co-infection rates with genogroup 1 TTV and HPV were significantly higher in oral squamous cell cancer than in controls, oral lichen planus or oral leukoplakia patients (12.3%, 0.0%, 6.7%, and 4.5%, respectively). The prevalence of genogroup 1 TTV in all cervical samples were comparable. These data suggest that genogroup 1 TTV may be associated specifically with some head and neck mucosal disorders, but disproves a (co)carcinogenic role inoral canceror cervical cancer aswell as an association with HPV or with malignancies associated with HPV.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
human papillomavirus
torque tenovirus
egyetemen (Magyarországon) készült közlemény
Megjelenés:Journal of Medical Virology. - 81 : 11 (2009), p. 1975-1981. -
További szerzők:Gáll Tamás (1982-) (molekuláris biológus, mikrobiológus) Murvai Melinda (1976-) (molekuláris biológus, mikrobiológus) Kis Andrea (1981-) (molekuláris biológus, mikrobiológus) Boda Róbert (1978-) (fogszakorvos) Sápy Tamás (1970-) (szülész-nőgyógyász) Tar Ildikó (1967-) (fogszakorvos) Gergely Lajos (1940-) (szakorvos, klinikai mikrobiológus) Szarka Krisztina (1971-) (molekuláris biológus, mikrobiológus)
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3.

001-es BibID:BIBFORM031960
Első szerző:Fehér Enikő (molekuláris biológus, mikrobiológus)
Cím:Comparison of diversity of torque teno virus 1 in different mucosal tissues and disorders / Fehér Enikő, Kardos Gábor, Gáll Tamás, Kis Andrea, Gergely Lajos, Szarka Krisztina
Dátum:2011
ISSN:1217-8950
Megjegyzések:Diversity of TTV1 was assessed in the head and neck region in patients with potentially malignant (oral lichen planus, oral leukoplakia) and malignant lesions (oral and laryngeal squamous cell cancers) and was compared to that found in the uterine cervix (cervical atypia and cervical cancer) by directly sequencing the NG061-063 segment of ORF1. These sequences were classified by the formerly used genogroupgenotype system as well as by the newly accepted species classification by aligning with the corresponding region of the type sequences of the 29 TTV species. All sequences obtained during the study clustered together with the TTV1 type sequence; to express diversity within TTV1, genotypes and subtypes of the former classification were used.The commonest subtypes were 2c followed by 2b, 1a and 1b. Subtypes 2b and 2c were evenly distributed among cervical samples; subtype 1a was more frequent in patients with cervical atypia or cancer. Subtypes 2c was more frequent than 2b in head and neck lesions. In conclusion, genotype and even subtype distribution may be important in association with diseases, therefore using this classification for characterization of intraspecies diversity of TTV1 is proposed.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény hazai lapban
Megjelenés:Acta Microbiologica et Immunologica Hungarica 58 : 4 (2011), p. 319-337. -
További szerzők:Kardos Gábor (1974-) (szakorvos, klinikai mikrobiológus) Gáll Tamás (1982-) (molekuláris biológus, mikrobiológus) Kis Andrea (1981-) (molekuláris biológus, mikrobiológus) Gergely Lajos (1940-) (szakorvos, klinikai mikrobiológus) Szarka Krisztina (1971-) (molekuláris biológus, mikrobiológus)
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4.

001-es BibID:BIBFORM047703
035-os BibID:PMID:23649705
Első szerző:Gáll Tamás (molekuláris biológus, mikrobiológus)
Cím:Genomic differences in the background of different severity in juvenile-onset respiratory papillomatoses associated with human papillomavirus type 11 / Tamás Gáll, Andrea Kis, Tímea Zsófia Tatár, Gábor Kardos, Lajos Gergely, Krisztina Szarka
Dátum:2013
ISSN:0300-8584
Megjegyzések:This study aimed to compare complete genome sequences of human papillomavirus (HPV) type 11 from two solitary papillomas (considered minimally aggressive), two moderately (six and nine episodes) and two highly aggressive (30 and 33 episodes) juvenile-onset respiratory papillomatoses. Genomic regions were sequenced using the Sanger method; sequences were compared to available GenBank genomes. Activity of the long control region (LCR) was assessed in HEp-2 cell line using luciferase assays and compared to that of the reference (GenBank Accession Number M14119). Site-directed mutagenesis was performed to confirm the association of polymorphisms with differences in LCR activity. Eleven alterations resulted in amino acid changes in different open reading frames. A72E in E1 and Q86K in E2 proteins were exclusively present in a moderately aggressive disease, L1 alterations A476V and S486F were unique to a severe papillomatosis. HPV11s in both solitary papillomas had identical LCRs containing a T7546C polymorphism, which strongly attenuated LCR activity, as confirmed by site-directed mutagenesis. This strong attenuator polymorphism was also present in the other four genomes showing significantly higher activities, but in these other alterations with demonstrable but statistically not significant attenuating (A7413C, 7509 T deletion) or enhancing (C7479T, T7904A) effect on transactivating potential (as demonstrated by site-directed mutagenesis) were also detected. LCR activities corresponded well to severity, excepting the highly aggressive papillomatosis with the L1 alterations. Presence of intratypic variants cannot explain differences in severity of respiratory papillomatoses associated with HPV11; virulence seems to be determined by the interaction of multiple genetic differences.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Medical Microbiology and Immunology. - 202 : 5 (2013), p. 353-363. -
További szerzők:Kis Andrea (1981-) (molekuláris biológus, mikrobiológus) Tatár Tímea Zsófia Kardos Gábor (1974-) (szakorvos, klinikai mikrobiológus) Gergely Lajos (1940-) (szakorvos, klinikai mikrobiológus) Szarka Krisztina (1971-) (molekuláris biológus, mikrobiológus)
Pályázati támogatás:OTKA 73145
OTKA
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5.

001-es BibID:BIBFORM024960
Első szerző:Gáll Tamás (molekuláris biológus, mikrobiológus)
Cím:Virological failure of intralesional cidofovir therapy in recurrent respiratory papillomatosis is not associated with genetic or epigenetic changes of HPV11 : complete genome comparison of sequential isolates / Gáll Tamás, Kis Andrea, Fehér Enikő, Gergely Lajos, Szarka Krisztina
Dátum:2011
ISSN:0166-3542
Megjegyzések:Five sequential human papillomavirus type 11 (HPV11) positive samples collected from an aggressive juvenile onset recurrent respiratory papillomatosis before, during and after intralesional cidofovir therapy leading to virological failure after initial response were analyzed. Sequencing of the complete genome as well as methylation analysis by bisulfate modification and sequencing of the long control region (LCR) were performed to seek for genetic and epigenetic changes as a possible background for therapy failure. Single-strand conformation polymorphism of E1, E2, E6, E7 and LCR was used to exclude the presence of multiple HPV11 infection. All five complete genomes were identical and all four E2 binding sites in the LCR were uniformly unmethylated in all five genomes. Thus the virological failure was not due to virological factors suggesting that cidofovir action may depend more heavily on the host.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Antiviral Research. - 92 : 2 (2011), p. 356-358. -
További szerzők:Kis Andrea (1981-) (molekuláris biológus, mikrobiológus) Fehér Enikő (1981-) (molekuláris biológus, mikrobiológus) Gergely Lajos (1940-) (szakorvos, klinikai mikrobiológus) Szarka Krisztina (1971-) (molekuláris biológus, mikrobiológus)
Pályázati támogatás:OTKA K73145
OTKA
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6.

001-es BibID:BIBFORM051684
035-os BibID:PMID:24710824
Első szerző:Kis Andrea (molekuláris biológus, mikrobiológus)
Cím:Frequency of Genetic and Epigenetic Alterations of p14ARF and p16INK4A in Head and Neck Cancer in a Hungarian Population / Andrea Kis, Tímea Zsófia Tatár, Tamás Gáll, Róbert Boda, Ildikó Tar, Tamás Major, Pál Redl, Lajos Gergely, Krisztina Szarka
Dátum:2014
ISSN:1219-4956 1532-2807
Megjegyzések:Occurrence of genetic and epigenetic alterations affecting p14ARF and p16INK4A were investigated in tumour samples of 37 oral (OSCC) and 28 laryngeal squamous cell cancer (LSCC) patients, and compared to exfoliated buccal epithelial cells of 68 healthy controls. Presence of deletions and mutations/polymorphisms affecting exons were examined using sequencing. Methylation status of promoters was assessed by methylation-specific PCR. Chi-square and Fisher's exact tests were used to compare frequency of events. Exon deletions were found in four controls, one OSCC and 22 LSCC patients; the latter significantly differed from controls (p<0.001). Only two mutations (T24610A and C24702A) were in p16 exon 1 of two OSCC patients. Polymorphisms G28575A (Ala140Thr), G31292C (C540G) and G28608A were found in both patient groups. The p14 promoter was unmethylated in 86.7 % of OSCC and in 85.7 % of LSCC patients; for the p16 promoter these rates were 69.0 % and 76.2 % for OSCC and LSCC patients, respectively. Combining the two patient groups, unmethylated promoter was significantly less frequent in case of both p14 and p16 (p=0.043 and p=0.001, respectively) compared to the control group. In summary, exon deletion may be important in LSCC, while promoter methylation was relatively frequent in both patient groups.
Tárgyszavak:Orvostudományok Egészségtudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Pathology & Oncology Research. - 20 : 4 (2014), p. 923-929. -
További szerzők:Tatár Tímea Zsófia Gáll Tamás (1982-) (molekuláris biológus, mikrobiológus) Boda Róbert (1978-) (fogszakorvos) Tar Ildikó (1967-) (fogszakorvos) Major Tamás (1973-) (fül-orr-gégész) Redl Pál (1953-) (szájsebész) Gergely Lajos (1940-) (szakorvos, klinikai mikrobiológus) Szarka Krisztina (1971-) (molekuláris biológus, mikrobiológus)
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7.

001-es BibID:BIBFORM008929
Első szerző:Kis Andrea (molekuláris biológus, mikrobiológus)
Cím:Epstein-Barr virus prevalence in oral squamous cell cancer and in potentially malignant oral disorders in an eastern Hungarian population / Kis Andrea, Fehér Enikő, Gáll Tamás, Tar Ildikó, Boda Róbert, D. Tóth Etelka, Méhes Gábor, Gergely Lajos, Szarka Krisztina
Dátum:2009
Megjegyzések:We tested 65, 44, and 116 patients with oral squamous cell cancer (OSCC), oral leukoplakia (OL), and oral lichen planus (OLP) against 68 age-matched controls for the presence of Epstein-Barr virus (EBV). Apparently healthy mucosa was simultaneously sampled and examined in all patients. Paraffin-embedded tissue sections of all EBV-positive patients with OSCC were examined for latent membrane protein-1 (LMP-1) expression (demonstrable in most EBV-associated malignancies) using immunohistochemistry. The prevalence of EBV in the controls and in OSCC, OL, and OLP lesions was 19.1%, 73.8%, 29.5%, and 46.6%, respectively, and 66.2%, 22.7%, and 31.9% in the healthy mucosa of patients, respectively. The prevalence of EBV in OSCC patients was significantly higher than in controls or in respective samples of the other two patient groups both in the lesion and in the healthy mucosa. Comparisons including only patients with EBV-negative lesions yielded similar results. Lesions of patients with OLP, but not of patients with OL, differed significantly from controls in EBV prevalence. In OSCC, LMP-1 expression was not detected, and EBV carriage was not significantly associated with any risk factors and did not influence the outcome. Although a high prevalence of EBV was found in OSCC, comparable carriage rates on healthy mucosa of patients indicated that an aetiological role of EBV is unlikely.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Epstein-Barr virus
egyetemen (Magyarországon) készült közlemény
aetiology
Megjelenés:European Journal of Oral Sciences. - 117 : 5 (2009), p. 536-540. -
További szerzők:Fehér Enikő (1981-) (molekuláris biológus, mikrobiológus) Gáll Tamás (1982-) (molekuláris biológus, mikrobiológus) Tar Ildikó (1967-) (fogszakorvos) Boda Róbert (1978-) (fogszakorvos) D. Tóth Etelka (1975-) (fogszakorvos) Méhes Gábor (1966-) (patológus) Gergely Lajos (1940-) (szakorvos, klinikai mikrobiológus) Szarka Krisztina (1971-) (molekuláris biológus, mikrobiológus)
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8.

001-es BibID:BIBFORM004816
Első szerző:Major Tamás (fül-orr-gégész)
Cím:Follow-up of HPV DNA copy number in cidofovir therapy of recurrent respiratory papillomatosis / Major Tamás, Sziklai István, Czeglédy Judit, Gáll Tamás, Gergely Lajos, Szarka Krisztina
Dátum:2008
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
human papillomavirus 11
cidofovir
Megjelenés:Anticancer Research. - 28 : 4B (2008), p. 2169-2174. -
További szerzők:Sziklai István (1954-) (fül-orr-gégész) Czeglédy Judit (1944-) (virológus) Gáll Tamás (1982-) (molekuláris biológus, mikrobiológus) Gergely Lajos (1940-) (szakorvos, klinikai mikrobiológus) Szarka Krisztina (1971-) (molekuláris biológus, mikrobiológus)
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9.

001-es BibID:BIBFORM008946
Első szerző:Szarka Krisztina (molekuláris biológus, mikrobiológus)
Cím:Progressive increase of human papillomavirus carriage rates in potentially malignant and malignant oral disorders with increasing malignant potential / Szarka Krisztina, Tar Ildikó, Fehér Enikő, Gáll Tamás, Kis Andrea, D. Tóth Etelka, Boda Róbert, Márton Ildikó, Gergely Lajos
Dátum:2009
Megjegyzések:We investigated the potential role of human papillomaviruses (HPVs) in potentially malignant oral disorders, oral leukoplakia (OL) and oral lichen planus (OLP), and in oral squamous cell cancer (OSCC) in an Eastern Hungarian population with a high incidence of OSCC. Methods: Excised tumor samples (65 OSCC patients) and exfoliated cells from potentially malignant lesions (from 44 and 119 patients with OL and OLP, respectively) as well as from healthy controls (72 individuals) were analysed. OLPs were classified based on clinical appearance, 61 patients had erosive?atrophic lesions (associated with higher malignancy risk, EA-OLP) and 58 had non-erosive non-atrophic lesions (with lower risk of becoming malignant, non-EA-OLP), respectively. Exfoliated cells collected from apparently healthy mucosa accompanied each lesion sample. HPV was detected by MY/GP polymerase chain reaction (PCR) and genotyped by restriction analysis of amplimers. Copy numbers in lesions were determined using real-time PCR. Prevalence rates, copy number distributions, and association with risk factors and diseases were analysed using chi-square test, t-test, and logistic regression, respectively. Results: We detected HPVs significantly more frequently in lesions than in controls (P ? 0.001 in all comparisons). HPV prevalence increased gradually with increasing severity of lesions (32.8, 40.9, and 47.7% in OLP, OL, and OSCC, respectively). Copy number distribution patterns roughly corresponded to prevalence rates, but OLP and OL were comparable. HPV prevalence differed significantly between EA-OLP and non-EA-OLP groups (42.6 vs. 22.4%); EA-OLP group showed a prevalence similar to that found in OL. Conclusion: HPVs may be involved in the development or progression of not only OSCC but also of potentially malignant oral lesions.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
human papillomavirus
oral carcinogenesis
Megjelenés:Oral Microbiology and Immunology 24 : 4 (2009), p. 314-318. -
További szerzők:Tar Ildikó (1967-) (fogszakorvos) Fehér Enikő (1981-) (molekuláris biológus, mikrobiológus) Gáll Tamás (1982-) (molekuláris biológus, mikrobiológus) Kis Andrea (1981-) (molekuláris biológus, mikrobiológus) D. Tóth Etelka (1975-) (fogszakorvos) Boda Róbert (1978-) (fogszakorvos) Márton Ildikó (1954-) (fogszakorvos) Gergely Lajos (1940-) (szakorvos, klinikai mikrobiológus)
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