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001-es BibID:	BIBFORM038169
Első szerző:	Kecskés Szilvia (anatómus, neurobiológus)
Cím:	Three-dimensional reconstruction and quantitative morphometric analysis of pyramidal and giant neurons of the rat dorsal cochlear nucleus / Kecskes Szilvia, Kőszeghy Áron, Szücs Géza, Rusznák Zoltán, Matesz Clara, Birinyi András
Dátum:	2013
ISSN:	1863-2653
Megjegyzések:	Correct interpretation of functional data obtained from various cell types of the cochlear nucleus (CN), a structure involved in auditory information processing, necessitates reliable cell identification. Our aim was to perform a quantitative morphological characterization of giant and pyramidal cells of the rat CN and identify parameters that are suitable for their adequate classification. Neurons were labeled with biocytin, visualized with a fluorescent marker, and three-dimensionally reconstructed from confocal images. The size and shape of the soma and dendritic tree of each neuron were characterized by 17 morphometric parameters. The variables were subjected to multivariate statistical analysis to determine their importance while discriminating between giant and pyramidal cells. Our results provide a new battery of morphometric data, which could not be obtained earlier, improve the chances of correct cell identification, make modeling experiments easier and more reliable, and help us to understand both the functions of individual CN neurons and the network properties of this nucleus. In addition, we demonstrate that even partial labeling and/or incomplete reconstruction of neurons may be enough for their correct identification if selected parameters describing the cell bodies and the proximal portions of the dendritic trees are utilized. We propose that our findings have specific relevance to studies which attempt cell identification after functional experiments resulting in incomplete labeling of the investigated neurons.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Brain Structure & Function. - 218 : 5 (2013), p. 1279-1292. -
További szerzők:	Kőszeghy Áron (1983-) (Ph.D hallgató, élettanász) Szűcs Géza (1948-) (élettanász) Rusznák Zoltán (1965-) (élettanász) Matesz Klára (1949-) (anatómus, neurobiológus) Birinyi András (1960-) (anatómus, neurobiológus)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM060025
Első szerző:	Kőszeghy Áron (Ph.D hallgató, élettanász)
Cím:	Endocannabinoid signaling modulates neurons of the pedunculopontine nucleus (PPN) via astrocytes. / Áron Kőszeghy, Adrienn Kovács, Tamás Bíró, Péter Szűcs, János Vincze, Zoltán Hegyi, Miklós Antal, Balázs Pál
Dátum:	2015
Megjegyzések:	The pedunculopontine nucleus (PPN) is known as the cholinergic part of the reticular activating system (RAS) and it plays an important role in transitions of slow-wave sleep to REM sleep and wakefulness. Although both exogenous and endocannabinoids affect sleep, the mechanism of endocannabinoid neuromodulation has not been characterized at cellular level in the PPN. In this paper, we demonstrate that both neurons and glial cells from the PPN respond to cannabinoid type 1 (CB1) receptor agonists. The neuronal response can be depolarization or hyperpolarization, while astrocytes exhibit more frequent calcium waves. All these effects are absent in CB1 gene-deficient mice. Blockade of the fast synaptic neurotransmission or neuronal action potential firing does not change the effect on the neuronal membrane potential significantly, while inhibition of astrocytic calcium waves by thapsigargin diminishes the response. Inhibition of group I metabotropic glutamate receptors (mGluRs) abolishes hyperpolarization, whereas blockade of group II mGluRs prevents depolarization. Initially active neurons and glial cells display weaker responses partially due to the increased endocannabinoid tone in their environment. Taken together, we propose that cannabinoid receptor stimulation modulates PPN neuronal activity in the following manner: active neurons may elicit calcium waves in astrocytes via endogenous CB1 receptor agonists. Astrocytes in turn release glutamate that activates different metabotropic glutamate receptors of neurons and modulate PPN neuronal activity.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Endocannabinoid Pedunculopontine nucleus CB1 receptor Neuromodulation Astrocyte Metabotropic glutamate receptor
Megjelenés:	Brain structure and function 220 : 5 (2015), p. 3023-3041. -
További szerzők:	Kovács Adrienn (1989-) (molekuláris biológus) Bíró Tamás (1968-) (élettanász) Szűcs Péter (1974-) (kutatóorvos) Vincze János (1988-) (orvos) Hegyi Zoltán (1983-) (molekuláris biológus) Antal Miklós (1951-) (orvos, anatómus) Pál Balázs (1975-) (élettanász)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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