Találati lista | Tudóstér

001-es BibID:	BIBFORM028740
Első szerző:	Keserű Judit (molekuláris genetikus)
Cím:	Investigation of beta-Lactamases in clinical isolates of Staphylococcus aureus for further explanation of borderline methicillin resistance / Keserű Judit Szilvia, Gál Zsuzsanna, Barabás György, Benkő Ilona, Szabó István
Dátum:	2005
ISSN:	0009-3157
Megjegyzések:	The borderline methicillin resistance in Staphylococcus aureus is due to ?-lactamase overproduction and/or specific methicillinases.Methods: beta-lactamase activity in culture supernatants and in cytoplasmic membrane fractions were estimated by bioassay and by SDS-PAGE combined with nitrocefin assay.Results: During the investigation of borderline methicillin-resistant Staphylococcus aureus (BORSA) strains VU94 and 822 two beta-lactamases were detected in the membranes, with molecular weights of 13 and 30 kDa. The second one could be found in the culture supernatants as well. In the presence of globomycin this enzyme disappeared from the membrane, and the oxacillin-hydrolysing activity of the membrane decreased to the level of susceptible strains. Both beta-lactamases were detected in the studied methicillin-resistant Staphylococcus aureus (MRSA) strain, but the susceptible strains possessed only the first enzyme.Conclusions: The 30 kDa ?-lactamase proved to be a methicillinase, and it can be one of the main causes of the borderline phenotype of BORSA strains. The other enzyme is one of the smallest published beta-lactamases until recently.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Chemotherapy. - 51 : 6 (2005), p. 300-304. -
További szerzők:	Gál Zsuzsanna (1959-) (vegyész) Barabás György (1933-) (sejtbiológus, molekuláris genetikus) Benkő Ilona (1954-) (orvos, farmakológus) Szabó István (1950-) (sejtbiológus, molekuláris genetikus)
Pályázati támogatás:	ETT 395/KO/03 Egyéb ETT T-08-313/2003 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon:

001-es BibID:	BIBFORM039004
035-os BibID:	PMID:19851727
Első szerző:	Penyige András (molekuláris genetikus)
Cím:	Analysis and identification of ADP-ribosylated proteins of Streptomyces coelicolor M145 / András Penyige, Judit Keseru, Ferenc Fazakas, Iván Schmelczer, Krisztina Szirák, György Barabás, Sándor Biró
Dátum:	2009
ISSN:	1225-8873
Megjegyzések:	Mono-ADP-ribosylation is the enzymatic transfer of ADP-ribose from NAD(+) to acceptor proteins catalyzed by ADP-ribosyltransferases. Using m-aminophenylboronate affinity chromatography, 2D-gel electrophoresis, in-gel digestion and MALDI-TOF analysis we have identified eight in vitro ADP-ribosylated proteins in Streptomyces coelicolor, which can be classified into three categories: (i) secreted proteins; (ii) metabolic enzymes using NAD(+)/NADH or NADP(+)/NADPH as coenzymes; and (iii) other proteins. The secreted proteins could be classified into two functional categories: SCO2008 and SC05477 encode members of the family of periplasmic extracellular solute-binding proteins, and SCO6108 and SC01968 are secreted hydrolases. Dehydrogenases are encoded by SC04824 and SC04771. The other targets are GlnA (glutamine synthetase I., SC02198) and SpaA (starvation-sensing protein encoded by SC07629). SCO2008 protein and GlnA had been identified as ADP-ribosylated proteins in previous studies. With these results we provided experimental support for a previous suggestion that ADP-ribosylation may regulate membrane transport and localization of periplasmic proteins. Since ADP-ribosylation results in inactivation of the target protein, ADP-ribosylation of dehydrogenases might modulate crucial primary metabolic pathways in Streptomyces. Several of the proteins identified here could provide a strong connection between protein ADP-ribosylation and the regulation of morphological differentiation in S. coelicolor.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban protein ADP-ribosylation Streptomyces coelicolor MALDI-TOF 2-D PAGE egyetemen (Magyarországon) készült közlemény
Megjelenés:	Journal Of Microbiology. - 47 : 5 (2009), p. 549-556. -
További szerzők:	Fazakas Ferenc (1969-) (molekuláris biológus) Schmelczer Iván Keserű Judit (1976-) (molekuláris genetikus) Szirák Krisztina (1973-) (molekuláris genetikus) Barabás György (1933-) (sejtbiológus, molekuláris genetikus) Biró Sándor (1949-) (molekuláris genetikus)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon:

001-es BibID:	BIBFORM039003
035-os BibID:	PMID:22752904
Első szerző:	Szirák Krisztina (molekuláris genetikus)
Cím:	Disruption of SCO5461 gene coding for a mono-ADP-ribosyltransferase enzyme produces a conditional pleiotropic phenotype affecting morphological differentiation and antibiotic production in Streptomyces coelicolor / Krisztina Szirák, Judit Keserű, Sándor Biró, Iván Schmelczer, György Barabás, András Penyige
Dátum:	2012
ISSN:	1225-8873
Megjegyzések:	The SCO5461 gene of Streptomyces coelicolor A3(2) codes for an ADP-ribosyltransferase enzyme that is predicted to be a transmembrane protein with an extracellular catalytic domain. PCR-targeted disruption of the gene resulted in a mutant that differentiated normally on complex SFM medium; however, morphological differentiation in minimal medium was significantly delayed and this phenotype was even more pronounced on osmotically enhanced minimal medium. The mutant did not sporulate when it was grown on R5 medium, however the normal morphological differentiation was restored when the strain was cultivated beside the wild-type S. coelicolor M145 strain. Comparison of the pattern of ADP-ribosylated proteins showed a difference between the mutant and the wild type, fewer modified proteins were present in the cellular crude extract of the mutant strain. These results support our previous suggestions that protein ADP-ribosylation is involved in the regulation of differentiation and antibiotic production and secretion in Streptomyces.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban Streptomyces egyetemen (Magyarországon) készült közlemény mono-ADP-ribosyltransferase protein ADP-ribosylation morphological differentiation actinorhodin production and secretion
Megjelenés:	Journal of Microbiology. - 50 : 3 (2012), p. 409-418. -
További szerzők:	Schmelczer Iván Keserű Judit (1976-) (molekuláris genetikus) Biró Sándor (1949-) (molekuláris genetikus) Barabás György (1933-) (sejtbiológus, molekuláris genetikus) Penyige András (1954-) (molekuláris genetikus)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon: