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001-es BibID:	BIBFORM037606
Első szerző:	Hajdu Péter (biofizikus)
Cím:	Answer to the "comment on functional consequences of Kv1.3 ion channel rearrangement into the immunological synapse" by Stefan Bittner et al. [Immunol. Lett. 125 (Aug 15 (2)) (2009) 156-157] / Hajdú, Péter, Szilágyi, Orsolya, Tóth, Ágnes, Krasznai, Zoltán, Pocsai, Krisztina, Panyi, György
Dátum:	2010
ISSN:	0165-2478
Tárgyszavak:	Orvostudományok Elméleti orvostudományok szerkesztői levél egyetemen (Magyarországon) készült közlemény
Megjelenés:	Immunology Letters. - 129 : 1 (2010), p. 47-49. -
További szerzők:	Szilágyi Orsolya (1985-) (molekuláris biológus, biokémikus) Tóth Ágnes (1983-) (biofizikus) Krasznai Zoltán (1950-) (biofizikus) Pocsai Krisztina (1978-) (élettanász) Panyi György (1966-) (biofizikus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat Szerző által megadott URL DOI
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001-es BibID:	BIBFORM037605
Első szerző:	Tóth Ágnes (biofizikus)
Cím:	Functional consequences of Kv1.3 ion channel rearrangement into the immunological synapse / Ágnes Tóth, Orsolya Szilágyi, Zoltán Krasznai, György Panyi, Péter Hajdú
Dátum:	2009
ISSN:	0165-2478
Megjegyzések:	Formation of immunological synapse (IS), the interface between T cells and antigen presenting cells, is a crucial step in T cell activation. This conjugation formation results in the rearrangement and segregation of a set of membrane bound and cytosolic proteins, including that of the T cell receptor, into membrane domains. It was showed earlier that Kv1.3, the dominant voltage-gated potassium channel of T cells redistributes into the IS on interaction with its specific APC. In the present experiments we investigated the functional consequences of the translocation of Kv1.3 channels into the IS formed between mouse helper T (T(h)2) and B cells. Biophysical characteristics of whole-cell Kv1.3 current in standalone cells (c) or ones in IS (IS) were determined using voltage-clamp configuration of standard whole-cell patch-clamp technique. Patch-clamp recordings showed that the activation of Kv1.3 current slowed (tau(a,1s) 2.36 +/- 0.13 ms (n = 7): tau(a,c) = 1.36 +/- 0.06 ms (n = 18)) whereas the inactivation rate increased (tau(i,1S) = 263 +/- 29 ms (n = 7): tau(i,c) = 365 +/- 27 ms (n = 17)) in cells being in IS compared to the standalone cells. The equilibrium distribution between the open and the closed states of Kv1.3 (voltage-dependence of steady-state activation) was shifted toward the depolarizing potentials in T cells engaged into IS (V-1/2,V-1S = -20.9 +/- 2 mV (n = 7). V-1/2,V-c = -26.4 +/- 115 mV (n = 12)). Thus, segregation of Kv1.3 channels into the IS modifies the gating properties of the channels. Application of protein kinase (PK) inhibitors (PKC: GF109203X, PKA: H89, p56Lck: damnacanthal) demonstrated that increase in the inactivation rate can be explained by the dephosphorylation of the channel protein. However, the slower activation kinetics of Kv1.3 in IS is likely to be the consequence of the redistribution of the channels into distinct membrane domains.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Cells immunological synapse Kinetics mouse Potassium Proteins egyetemen (Magyarországon) készült közlemény
Megjelenés:	Immunology Letters. - 125 : 1 (2009), p. 15-21. -
További szerzők:	Szilágyi Orsolya (1985-) (molekuláris biológus, biokémikus) Krasznai Zoltán (1950-) (biofizikus) Panyi György (1966-) (biofizikus) Hajdu Péter (1975-) (biofizikus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
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001-es BibID:	BIBFORM037603
Első szerző:	Varga Zoltán (biofizikus, szakfordító)
Cím:	Potassium channel expression in human CD4(+) regulatory and naive T cells from healthy subjects and multiple sclerosis patients / Zoltan Varga, Tunde Csepany, Ferenc Papp, Akos Fabian, Peter Gogolak, Agnes Toth, Gyorgy Panyi
Dátum:	2009
ISSN:	0165-2478
Megjegyzések:	The membrane potential of human T cells is regulated by two potassium channels: the voltage-gatedKV1.3 and the Ca2+-activated KCa3.1. These two channels are essential for efficient antigenic activation andproliferation of T cells and are expressedat different levels in naïve, centralmemory and effectormemory Tcells. This provides the opportunity to inhibit the proliferation of the targeted subtype by channel-specificblocking compounds. Regulatory T cells (Tregs) also represent a unique subtype of T cells that performhighly specialized tasks in controlling immune responses, which raises the possibility that they too havea distinctive channel expression pattern. Using whole-cell patch-clamp we tested this hypothesis anddetermined the ion channel expression of CD4+CD25hiCD127lo regulatory and CD4+CD25loCD127hi naïveT cells from the peripheral blood of healthy volunteers and multiple sclerosis (MS) patients sorted by flowcytometry. We have found that naïve and Treg cells from healthy controls expressed equal numbers ofKV1.3 channels, while Tregs had a greater membrane surface as assessed by capacitance measurements,and consequentially lower channel density than naïve cells, indicating an "incomplete activation state"of Tregs. In contrast, Tregs from MS patients had fewer KV1.3 channels than naïve cells and there wasno difference in the membrane capacitance or channel density between the two subtypes of cells. Theexpression level of KCa3.1 channels was similar in all cell subsets. The observed differences in KV1.3channel expression density may contribute to the varying responses upon antigenic stimulation by thesecell types in health and disease.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Regulatory T cell Potassium channel Multiple sclerosis Cell capacitance Channel density egyetemen (Magyarországon) készült közlemény
Megjelenés:	Immunology Letters 124 : 2 (2009), p. 95-101. -
További szerzők:	Csépány Tünde (1956-) (neurológus, pszichiáter) Papp Ferenc (1979-) (biofizikus) Fábián Ákos István (1982-) (aneszteziológus) Gogolák Péter (1968-) (biológus, immunológus) Tóth Ágnes (1983-) (biofizikus) Panyi György (1966-) (biofizikus)
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