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001-es BibID:BIBFORM016922
035-os BibID:(WoS)000283477500003 (Scopus)77956231195
Első szerző:Csiba László (neurológus, pszichiáter)
Cím:Visualization of the ischemic core on native human brain slices by potassium staining method / Csiba Laszlo, Farkas Szabolcs, Kollar Jozsef, Berenyi Ervin, Nagy Katalin, Bereczki Daniel
Dátum:2010
ISSN:0165-0270
Megjegyzések:The potassium staining method is based on the formation of potassiumcobaltnitrite crystals after the treatment by sodium cobaltnitrite ofbrain tissue. The degree of staining correlates with the distinctpotassium content of infracted and non-infarcted brain areas.The aim of the present study was to prove that potassium stainingtechnique is a reliable method for localization of ischemic core onnative whole hemisphere cryosections of stroke patients. Furthermore,potassium stained sections have been compated with appropriatepostmortem MRI images of respective brains.Brains of stroke patients were removed within 24 h after death andpostmortem MRI scanning was performed. Horizontal cryosections offrozen brains were taken and potassium staining was performed. Usingthe stained whole hemisphere sections as "map" tissue sampling has beenmade in order to determine water and potassium content.Potassium content of infarcted samples was significantly decreased incomparison with intact regions (0.7346 +/- 0.2142 mg/L and 1.928 +/-0.447 mg/L, respectively, p<0.01) (mean values +/-SD). Water content ofaffected areas (expressed in percents) has been found to be abovenon-infarcted regions (81.657% +/- 4.07 and 72.96% +/- 6.37,respectively, p<0.01).According to our results the potassium staining method of human wholehemisphere brain sections reliably differentiates focal ischemic areasfrom intact brain regions. In conclusion, the postmortem examination ofischemic brain could be started with making the potassium map ofinfarcted whole hemisphere cryosections providing guidance for targetedtissue sampling and base of comparison for further examinations. (C)2010 Elsevier B.V. All rights reserved.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Journal of Neuroscience Methods. - 192 : 1 (2010), p. 17-21. -
További szerzők:Farkas Szabolcs (1983-) (orvos) Kollár József (1950-) (radiológus) Berényi Ervin (1964-) (radiológus) Nagy Katalin (1960-) (orvosdiagnosztikai laboratóriumi analitikus) Bereczki Dániel (1960-) (neurológus)
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2.

001-es BibID:BIBFORM087867
035-os BibID:(Scopus)85091004594 (WOS)000565252800001
Első szerző:Fülesdi Béla (aneszteziológus)
Cím:Correlation between calcium, water contents and ultrasonographic appearance of atherosclerotic lesions of carotid artery lesions / Béla Fülesdi, Szabolcs Farkas, Zoltán Gyöngyösi, Péter Siró, Dániel Bereczki, József Bacsó, László Csiba
Dátum:2020
ISSN:2081-3856 2081-6936
Megjegyzések:Background We tested the hypothesis whether there is a correlation between the echogenicity and calcium and water contents of carotid plaques. Patients and methods Ninety carotid befurcations from 45 deceased patients were removed during autopsy. Thirty-four plaques were categorized as homogenous echolucent (HEL), homogenous echogenic (HEG) and heterogenous (HE) plaques based on premortem B-mode image. Water content was expressed in % of wet weight. Ca was determined by proton-induced X-ray emission and expressed in ppm. Relative optical density of the B-mode images was analyzed offline using a computer program. Results HEL plaques had lower Ca content (medians and IQRs: 6,145 [4,465?6,536?ppm]) compared to HEG (74,100 [15,300?1,44,500?ppm]), P ? 0.001). HE plaques showed an intermediate calcium content (7,310 [4,840?9,920?ppm]) that was statistically not different from echolucent plaques. Water content of HEG plaques was statistically not different from HEL and HE (HEG:53.5 [35.5?64%], HEL: 73.5 [69.7?78.5%], HE: 70.6 [67.4?73.9%]). HEG plaques had the highest relative optical densities (196 [188?217%]). HEL and HE had similar relative optical densities (HEL: 176 [164?187%], HE: 164 [144?188%], respectively). A significant positive correlation was found between the Ca content and relative optical density of plaques. Conclusions Echogenicity of carotid plaques increases along with their calcium content. Water content may be an important factor in differentiation of different plaques.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Ca and water contents
carotid plaque
B-mode ultrasound
ultrasonic tissue characterisation
Megjelenés:Translational Neuroscience. - 11 : 1 (2020), p. 269-276. -
További szerzők:Farkas Szabolcs (1983-) (orvos) Gyöngyösi Zoltán (1977-) (aneszteziológus) Siró Péter (1973-) (neurológus, aneszteziológus, intenzív terápiás szakorvos) Bereczki Dániel (1960-) (neurológus) Bacsó József Csiba László (1952-) (neurológus, pszichiáter)
Pályázati támogatás:2017-1.2.1-NKP-2017-00002
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Internet cím:Szerző által megadott URL
DOI
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