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001-es BibID:BIBFORM075465
035-os BibID:(WoS)000427563000023 (Scopus)85042127210
Első szerző:Jančaříková, Gita
Cím:Synthesis of α-L-fucopyranoside-presenting glycoclusters and investigation of their interaction with Photorhabdus asymbiotica lectin (PHL) / Gita Jančaříková, Mihály Herczeg, Eva Fujdiarová, Josef Houser, Katalin E. Kövér, Anikó Borbás, Michaela Wimmerová, Magdolna Csávás
Dátum:2018
ISSN:0947-6539 1521-3765
Megjegyzések:Photorhabdus asymbiotica is gram-negative bioluminescent bacteria that is not only as effective an insect pathogen as other members of the genus, but also a bacterium that causes serious diseases in humans. The recently identified bangle lectin PHL from P. asymbiotica verifiably modulates an immune response of humans and insects, which supports the idea that the lectin might play an important role in the host-pathogen interaction. Dimeric PHL contains up to seven L-fucose specific binding sites per monomer, and in order to target multiple binding sites of PHL, alpha-L-fucoside-containing di-, tri- and tetravalent glycoclusters were synthesized. Methyl gallate and pentaerythritol were chosen as multivalent scaffolds, and the fucoclusters were built from the above-mentioned cores by coupling with different oligoethylene bridges and propargyl ?-L-fucosides using 1,3-dipolar azide-alkyne cycloaddition. The interaction between fucoside derivates and PHL was investigated by several biophysical and biological methods, ITC and SPR measurements, hemagglutination inhibition assay and an investigation of bacterial aggregation properties were carried out. Moreover, details of the interaction between PHL and propargyl alpha-L-fucoside as a monomer unit were revealed using X-ray crystallography. Besides this, the interaction with multivalent compounds was studied by NMR techniques. The newly synthesized multivalent fucoclusters proved to be up to several orders of magnitude better ligands than the natural ligand, L-fucose.
Tárgyszavak:Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
fucoclusters
lectin
multivalency
Photorhabdus asymbiotica
agglutination
Megjelenés:Chemistry-A European Journal. - 24 : 16 (2018), p. 4055-4068. -
További szerzők:Herczeg Mihály (1979-) (vegyész, biológia-kémia tanár) Fujdiarová, Eva Houser, Josef (1977-) (vegyész) Kövér Katalin, E. (1956-2023) (vegyész) Borbás Anikó (1965-) (vegyész) Wimmerová, Michaela Csávás Magdolna (1977-) (szénhidrátkémikus, vegyész)
Pályázati támogatás:NKFIH K119509
NKFIH
GINOP-2.3.2-15-2016-00008
GINOP
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
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2.

001-es BibID:BIBFORM082132
035-os BibID:(cikkazonosító)686 (WoS)000502267900047 (Scopus)85074535772
Első szerző:Malinovská, Lenka
Cím:Synthesis of [beta]-D-galactopyranoside-Presenting Glycoclusters, Investigation of Their Interactions with Pseudomonas aeruginosa Lectin A (PA-IL) and Evaluation of Their Anti-Adhesion Potential / Lenka Malinovská, Son Thai Le, Mihály Herczeg, Michaela Vasková, Josef Houser, Eva Fujdiarová, Jan Komárek, Petr Hodek, Anikó Borbás, Michaela Wimmerová, Magdolna Csávás
Dátum:2019
ISSN:2218-273X
Megjegyzések:Pseudomonas aeruginosa is an opportunistic human pathogen associated with cystic fibrosis. This bacterium produces, among other virulence factors, a soluble d-galactose-specific lectin PA-IL (LecA). PA-IL plays an important role in the adhesion to the host cells and is also cytotoxic. Therefore, this protein is an interesting therapeutic target, suitable for inhibition by carbohydrate-based compounds. In the current study, β-d-galactopyranoside-containing tri- and tetravalent glycoclusters were synthesized. Methyl gallate and pentaerythritol equipped with propargyl groups were chosen as multivalent scaffolds and the galactoclusters were built from the above-mentioned cores by coupling ethylene or tetraethylene glycol-bridges and peracetylated propargyl β-d-galactosides using 1,3-dipolar azide-alkyne cycloaddition. The interaction between galactoside derivatives and PA-IL was investigated by several biophysical methods, including hemagglutination inhibition assay, isothermal titration calorimetry, analytical ultracentrifugation, and surface plasmon resonance. Their ability to inhibit the adhesion of P. aeruginosa to bronchial cells was determined by ex vivo assay. The newly synthesized multivalent galactoclusters proved to be significantly better ligands than simple d-galactose for lectin PA-IL and as a result, two representatives of the dendrimers were able to decrease adhesion of P. aeruginosa to bronchial cells to approximately 32% and 42%, respectively. The results may provide an opportunity to develop anti-adhesion therapy for the treatment of P. aeruginosa infection.
Tárgyszavak:Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Pseudomonas aeruginosa
cystic fibrosis
lectin
d-galactosides
multivalency
Megjelenés:Biomolecules. - 9 : 11 (2019), p. 1-22. -
További szerzők:Le Thai, Son (1990-) (gyógyszerész) Herczeg Mihály (1979-) (vegyész, biológia-kémia tanár) Vaskova, Michaela Houser, Josef (1977-) (vegyész) Fujdiarová, Eva Komárek, Jan Hodek, Petr Borbás Anikó (1965-) (vegyész) Wimmerová, Michaela Csávás Magdolna (1977-) (szénhidrátkémikus, vegyész)
Pályázati támogatás:NKFIH K 119509
Egyéb
GINOP-2.3.2-15-2016-00008
GINOP
ÚNKP-19-4
Egyéb
PPD 461038
Egyéb
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
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