CCL

Összesen 3 találat.
#/oldal:
Részletezés:
Rendezés:

1.

001-es BibID:BIBFORM062409
Első szerző:Fodor János (élettanász, biotechnológus)
Cím:The effect of follistatin on the calcium homeostasis and differentiation of the C2C12 skeletal muscle cells / Fodor János, Tóth Adrienn, Vincze János, Oláh Tamás, Dienes Beatrix, Zádor Ernő, Csernoch László
Dátum:2015
Megjegyzések:Myostatin (MSTN), a member of the transforming growth factor b superfamily has emerged as a negative regulator of skeletalmuscle growth. During embryogenesis, myostatin is exclusively expressed in skeletal muscle to control the differentiation and proliferation of the myoblasts. It mediates the cell signaling cascade through activin receptors in the muscle. Follistatin (FS) is a high affinity activin-binding protein that can act as an activin antagonist. In our experiments we applied FS and the effect of the elimination of activin A signaling pathway on the Ca2+-homeostasis and the expression pattern of the key proteins involved in the differentiation of C2C12 skeletal muscle cells was studied. Functional experiments were performed on differentiated C2C12 myotubes by measuring the changes in [Ca2+]i following the stimulation by KCl or caffeine. The amplitude of the caffeine-induced Ca2+-transients were significantly higher in FS-treated cells (378 ? 32 nM) as compared to control (241 ? 30; p = 0.004;) in the presence of normal [Ca2+]e.While the application of KCl did not modify the amplitude of the Ca2+-transients, the Ca2+-uptake capacity of the sarcoplasmic reticulum assessed as the uptake rate of the calciumpumps (SERCA) significantly increased (340 ? 16 lM/s vs. 280 ? 11 lM/s; p = 0.007 in FS-treated and control cells, respectively). Following FS treatment changes in expression of SERCA were also detected. Additionally, expression of MSTN, Akt and P-Akt were significantly altered during differentiation. Our results suggest that the application of FS increased the Ca2+-influx via store operated calcium entry when RyRs were activated with caffeine. Furthermore, the altered MSTN and P-Akt expression could be responsible for the increased muscle differentiation. This work was supported by Grants: OTKA NN-107765, TÁMOP-4.2.1/B-09/1/KONV-2010-0007 and TÁMOP-4.2.2/B-10/1-2010-0024, Bólyai Research Scholarship of the Hungarian Academy of Sciences to J.F.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idézhető absztrakt
Myostatin
C2C12 cell
Follistatin
Megjelenés:Journal of muscle research and cell motility 36 (2015), p. 131. -
További szerzők:Tóth Adrienn (1988-) (molekuláris biológus, élettanász) Vincze János (1947-) (biofizikus) Oláh Tamás (1983-) (élettanász) Dienes Beatrix (1972-) (élettanász, molekuláris biológus) Zádor Ernő (1954-) Csernoch László (1961-) (élettanász)
Pályázati támogatás:NN-107765
OTKA
TÁMOP-4.2.1/B-09/1/KONV-2010-0007
TÁMOP
A harántcsíkolt izom kontrakció molekuláris szabályozása
TÁMOP-4.2.2/B-10/1-2010-0024
TÁMOP
Molekuláris Orvostudomány Doktori Iskola
Internet cím:DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

2.

001-es BibID:BIBFORM062408
Első szerző:Oláh Tamás (élettanász)
Cím:CB1 cannabinoid receptors are involved in the regulation of excitation-contraction coupling in mammalian skeletal muscle / Oláh Tamás, Bodnár Dóra, Tóth Adrienn, Fodor János, Kovács Adrienn, Farkas Anna, Nádró Bíborka, Szentesi Péter, Csernoch László
Dátum:2015
Megjegyzések:The presence of CB1 cannabinoid receptors (CB1R) has been shown in skeletal muscle, but it is yet to be cleared whether they have any significance in the regulation of contractions. CB1-knockout (CB1-KO) mice showed hypoactivity, however, it is questionable whether this was solely due to effects on the central nervous system or impairment of muscle function also contributes. Our aim was to investigate the role of CB1R in mammalian skeletal muscle, and the effects of cannabinoid drugs on Ca2+ transients. Running ability of control and CB1-KO mice was studied by activity-wheel-tests while in vivo muscle force of the animals was measured by grip-tests and hang-tests. Ca2+ transients evoked by KCl-depolarization in the presence and absence of cannabinoid agonists were investigated on flexor digitorum brevis (FDB) fibers of control and CB1-KO mice. CB1-KO mice performed worse as compared to control in all behavior tests applied. In contrast, depolarization-evoked Ca2+ transients were significantly higher in FDB fibers isolated from CB1-KO mice (848 ? 98 nM, n = 47) compared to control (376 ? 60 nM, n = 32, p\0.01). When KCl-evoked Ca2+ transients were repeated on control FDB fibers in the absence and presence of the CB1 agonist WIN55,212 (WIN), the transients after WIN treatment were significantly smaller (44 ? 7 % of the first transient, n = 27) than in untreated (79 ? 5 % of the first transient, n = 32, p\0.01) fibers. Our observations suggest that CB1R-mediated signaling contributes to the regulation of excitation?contraction coupling and skeletal muscle contractions. Nevertheless, the effects mediated by the absence of CB1R in the central nervous system on the inferior muscle performance of CB1-KO mice in vivo cannot be ruled out. These results can contribute to the identification of the side effects of medically used cannabinoid drugs on skeletal muscle.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idézhető absztrakt
Skeletal muscle
CB1 cannabinoid receptor
Excitation?contraction coupling
Megjelenés:Journal of muscle research and cell motility. - 36 (2015), p. 129. -
További szerzők:Bodnár Dóra (1987-) (molekuláris biológus) Tóth Adrienn (1988-) (molekuláris biológus, élettanász) Fodor János (1973-) (élettanász, biotechnológus) Kovács Adrienn (1989-) (molekuláris biológus) Farkas Anna (1983-) Nádró Bíborka (1992-) (általános orvos) Szentesi Péter (1967-) (élettanász) Csernoch László (1961-) (élettanász)
Pályázati támogatás:TÁMOP-4.2.4.A/2-11-1-2012-0001
TÁMOP
Internet cím:DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

3.

001-es BibID:BIBFORM062410
Első szerző:Tóth Adrienn (molekuláris biológus, élettanász)
Cím:Investigation of the role of SERCA1b in the calcium homeostasis of C2C12 skeletal muscle cells / Tóth Adrienn, Fodor János, Vincze János, Oláh Tamás, Juhász Tamás, Zádor Ernő, Csernoch László
Dátum:2015
Megjegyzések:The neonatal isoform of the sarcoplasmic/endoplasmic reticulum Ca2+ ATPase 1 (SERCA1b) is a dominant Ca2+ pump in fibers of regenerating muscle. Our aim was to study the role of SERCA1b during skeletal muscle differentiation and to provide a detailed understanding of role in Ca2+ homeostasis. SERCA1b protein synthesis has been suppressed using a specific shRNA sequence. Clones with decreased SERCA 1b expression were selected for additional experiments. Scrambled shRNA transfected and parental cells were used as controls. Of the main regulatory proteins, expressions of phosphoprotein kinase B (P-Akt), myogenic differentiation factor (MyoD), stromal interaction molecule 1 (STIM1), calsequestrin (CSQ), and calcineurin were confirmed at the protein level, while myostatin and myocyte-enriched calcineurin-interacting protein (MCIP1.4) were identified at mRNA level. Quantitative analysis of the Western blots normalized to actin also confirmed the significant alterations in CSQ, STIM1, P-Akt, and calcineurin expression detected in the clones as compared to control. Furthermore, to examine the functional consequences of the decreased expression of SERCA1b, repeated Ca2+-transients were evoked by the applications of 120 mM KCl. Significantly higher [Ca2+]i was measured in the 20th and 40th seconds after the beginning of KCl application (112.3 ? 3.2 nM, and 110.4 ? 3.1 vs. 150.3 ? 6.5 nM and 134.9 ? 4.7, in control and in the clone C1 respectively) indicating decreased Ca2+ uptake capability of the SERCA pumps. This maximal pump rate was 453.6 ? 41.01 vs. 143.9 ? 24.01 lM/s, in control and in the clone C1. These results suggest that SERCA1b plays an essential role in the regulation of [Ca2+]i and ab ovo gene silencing results in decreased skeletal muscle differentiation. This work was supported by Grants: OTKA NN-107765, TÁMOP-4.2.1/B-09/1/KONV-2010-0007 and TÁMOP-4.2.2/B-10/1-2010-0024, Bólyai Research Scholarship of the Hungarian Academy of Sciences to J.F.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idézhető absztrakt
C2C12 cell
SERCA1b
Calcium homeostasis
Molekuláris Medicina
Doktori iskola
Megjelenés:Journal of muscle research and cell motility. - 36 (2015), p. 132. -
További szerzők:Fodor János (1973-) (élettanász, biotechnológus) Vincze János (1947-) (biofizikus) Oláh Tamás (1983-) (élettanász) Juhász Tamás (1976-) (biológus, orvosbiológus) Zádor Ernő (1954-) Csernoch László (1961-) (élettanász)
Pályázati támogatás:NN-107765
OTKA
TÁMOP-4.2.1/B-09/1/KONV-2010-0007
TÁMOP
A harántcsíkolt izom kontrakció molekuláris szabályozása
TÁMOP-4.2.2/B-10/1-2010-0024
TÁMOP
Molekuláris Orvostudomány Doktori Iskola
Bólyai Research Scholarship of the Hungarian Academy of Sciences to J.F
Egyéb
Internet cím:DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Rekordok letöltése1