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001-es BibID:BIBFORM061520
035-os BibID:(WoS)000371800000009 (Scopus)84960539521
Első szerző:Csikós Ádám (molekuláris biológus)
Cím:Applicability and sensitivity of PCR-SSCP method for milk species identification in cheese / A. Csikós, A. Hodzic, E. Pasic-Juhas, A. Jávor, A. Hrkovic-Porobija, T. Goletic, G. Gulyás, L. Czeglédi
Dátum:2016
ISSN:0139-3006 1588-2535
Megjegyzések:Species identification in food has become a prominent issue in recent years as the importance of consumer protection has increased. DNA-based species identification methods were developed by researchers in the last two decades, as these are reliable, accurate, and low-cost techniques for species identification in raw and processed food products as well. In our study, universal primers were designed to conserved regions of mitochondrial 12S rRNA. Amplicons were heat-denatured and a PCR single strand conformation polymorphism (SSCP) method was developed to identify cattle, buffalo, sheep, and goat DNA. Sensitivity of this technique was tested on DNA mixtures of cattle-sheep, cattle-goat, and cattle-buffalo and the threshold limit of cattle DNA was 5%, 5%, and 3%, respectively. One hundred and five cheeses were purchased and collected from Bosnian and Hungarian farmers, retails, and supermarkets to reveal fraud, 32 percent of them (34 cheeses) were found to be mislabelled by species.
Tárgyszavak:Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény hazai lapban
folyóiratcikk
Megjelenés:Acta Alimentaria. - 45 : 1 (2016), p. 69-76. -
További szerzők:Hodzic, Aida Pasic-Juhas, Eva Jávor András (1952-) (agrármérnök) Hrkovic-Porobija, Amina Goletic, Teufik Gulyás Gabriella (1985-) (agrár) Czeglédi Levente (1977-) (agrármérnök)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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2.

001-es BibID:BIBFORM085927
Első szerző:Gulyás Gabriella (agrár)
Cím:Proteomic analysis of skeletal muscle at different live weights in Charolais bulls / Gulyás G., Czeglédi L., Béri B., Harangi S., Csősz E., Szabó Z.. Janáky T., Jávor A.
Dátum:2015
ISSN:0139-3006 1588-2535
Megjegyzések:The aim of this study was to investigate the differences in the proteome patterns of musculus longissimus dorsi between Charolais bulls slaughtered at 500 kg and 700 kg live weight using two-dimensional difference in gel electrophoresis (2D-DIGE) and liquid chromatography-mass spectrometry (LC-MS). Three hundred fi fty protein spots were visualised on gels of which 10 showed different expression levels (P<0.05) between groups. After mass spectrometric analysis of spots, beta-enolase (ENO3) in fi ve different spots, alpha-enolase (ENO1), triosephosphate isomerase (TPI1) in two different spots, alpha-actin (ACTA1), and heat shock protein beta-1 (HSPB1) were identifi ed. ENO3, ENO1, TPI1, and ACTA1 had higher expression levels in bulls of 700 kg live weight group. ENO3, ENO1, and TPI1 are involved in energy metabolism, while ACTA1 is a structure protein in skeletal muscle. Up-regulation of heat shock protein beta-1 (HSPB1), which protein is reported to have correlation with tenderness, was observed in 500 kg weight group. Our result demonstrates that proteomic tools are useful in identifying markers associated with muscle development.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény hazai lapban
folyóiratcikk
2D-DIGE
cattle
beef
slaughter weight
proteome
Megjelenés:Acta Alimentaria. - 44 : 1 (2015), p. 132-138. -
További szerzők:Czeglédi Levente (1977-) (agrármérnök) Béri Béla (1951-) (agrármérnök) Harangi Sándor (1982-) (agrármérnök) Csősz Éva (1977-) (biokémikus, molekuláris biológus) Szabó Zoltán (orvos, Szeged) Janáky Tamás Jávor András (1952-) (agrármérnök)
Pályázati támogatás:TÁMOP-4.2.1/B-09/1/KONV-2010-0007
TÁMOP
TÁMOP-4.2.2/B-10/1-2010-0024
TÁMOP
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