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001-es BibID:BIBFORM077512
Első szerző:Kristóf Endre (általános orvos)
Cím:Interleukin-6 released from differentiating human beige adipocytes improves browning / Endre Kristóf, Ágnes Klusóczki, Roland Veress, Abhirup Shaw, Zsolt Sándor Combi, Klára Varga, Ferenc Győry, Zoltán Balajthy, Péter Bai, Zsolt Bacso, László Fésüs
Dátum:2019
ISSN:0014-4827
Megjegyzések:Brown and beige adipocytes contribute significantly to the regulation of whole body energy expenditure and systemic metabolic homeostasis not exclusively by thermogenesis through mitochondrial uncoupling. Several studies have provided evidence in rodents that brown and beige adipocytes produce a set of adipokines ("batokines") which regulate local tissue homeostasis and have beneficial effects on physiological functions of the entire body. We observed elevated secretion of Interleukin (IL)-6, IL-8 and monocyte chemoattractant protein (MCP)-1, but not tumor necrosis factor alpha (TNFα) or IL-1β pro-inflammatory cytokines, by ex vivo differentiating human beige adipocytes (induced by either PPARγ agonist or irisin) compared to white. Higher levels of IL-6, IL-8 and MCP-1 were released from human deep neck adipose tissue biopsies (enriched in browning cells) than from subcutaneous ones. IL-6 was produced in a sustained manner and mostly by the adipocytes and not by the undifferentiated progenitors. Continuous blocking of IL-6 receptor by specific antibody during beige differentiation resulted in downregulation of brown marker genes and increased morphological changes that are characteristic of white adipocytes. The data suggest that beige adipocytes adjust their production of IL-6 to reach an optimal level for differentiation in the medium enhancing browning in an autocrine manner.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Experimental Cell Research. - 377 : 1-2 (2019), p. 47-55. -
További szerzők:Klusóczki Ágnes (1991-) (biotechnológus) Veress Roland (1992-) (molekuláris biológus) Shaw, Abhirup (1992-) Combi Zsolt Varga Klára Győry Ferenc (1969-) (kardiológus) Balajthy Zoltán (1957-) (biokémikus, sejtbiológus) Bai Péter (1976-) (biokémikus) Bacsó Zsolt (1963-) (biofizikus) Fésüs László (1947-) (orvos biokémikus)
Pályázati támogatás:GINOP-2.3.2-15-2016-00006
GINOP
OTKA-NK105046
OTKA
OTKA-K123975
OTKA
ÚNKP-18-4
ÚNKP
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
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2.

001-es BibID:BIBFORM066378
Első szerző:Kristóf Endre (általános orvos)
Cím:Differentiating human beige adipocytes secrete cytokines ("batokines") / Endre Kristóf, Ágnes Klusóczki, Zsolt Combi, Roland Veress, Ferenc Győry, Szilárd Póliska, Zsolt Bacsó, László Fésüs
Dátum:2016
Megjegyzések:Several studies highlighted the strong negative correlation between obesity and metabolically active brown adipose tissue amount in adult humans. There are at least two types of thermogenic fat depots, classical brown and beige, which have different origins and tissue distribution. Beige adipocytes contribute to the regulation of systemic metabolic homeostasis not exclusively by thermogenesis and mitochondrial uncoupling. In the present study, we investigated the secretion of "batokines" by primary human brown and beige adipocytes and by human adipose tissue samples. Adipose-derived mesenchymal stem cells obtained from herniotomy (abdominal subcutaneous) or thyroid surgery ("deep neck" and cervical subcutaneous) and a human preadipocyte cell line (SGBS) were differentiated into white, brown (by BMP7 treatment) or beige (by irisin administration or by a previously described cocktail) adipocytes. Cytokine gene expression in browning adipocytes was determined by RNA sequencing. Conditioned differentiation media were collected during the replacement of the adipogenic cocktails and secreted IL-6, IL-8, TNF?, MCP-1 and IL1-? were measured by ELISA. IL-6, MCP-1 and IL-8 secretion was significantly higher by beige compared to white adipocytes. In contrast to BMP7 administration (when classical brown adipocyte differentiation occurs), irisin treatment (which induces beige adipocyte differentiation) resulted in an increased total IL-6, MCP-1 and IL-8 production. Media collected daily or after three day periods contained the same amount of IL-6 depending only on the phase of differentiation. This suggests that adipocytes adjusted their production of the cytokine to reach an optimal level in the medium. In the heterogeneous population of differentiating adipocytes, IL-6 containing vesicles could be visualized mostly in beige cells by Laser-scanning cytometry. In the future we intend to explore the functional significance of the produced "batokines".
Tárgyszavak:Orvostudományok Elméleti orvostudományok idézhető absztrakt
Megjelenés:Biokémia. - 40 : 3 (2016), p. 37. -
További szerzők:Klusóczki Ágnes (1991-) (biotechnológus) Combi Zsolt Veress Roland (1992-) (molekuláris biológus) Győry Ferenc (1969-) (kardiológus) Póliska Szilárd (1978-) (biológus) Bacsó Zsolt (1963-) (biofizikus) Fésüs László (1947-) (orvos biokémikus)
Pályázati támogatás:TÁMOP-4.2.2.A-11/1/KONV-2012-0023
TÁMOP
TÁMOP-4.2.2.A-11/1/KONV-2012-0025
TÁMOP
TRANSCOM IAPP 251506
FP7
MTA-DE
MTA
Apoptózis és Genomika Kutatócsoport
NK105046
OTKA
GINOP-2.3.2-15-2016-00006
GINOP
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
Borító:
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