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001-es BibID:BIBFORM073611
035-os BibID:(WoS)000428382400019 (Scopus)85044544121
Első szerző:Hegyi Bence (élettanász)
Cím:Complex electrophysiological remodeling in postinfarction ischemic heart failure / Bence Hegyi, Julie Bossuyt, Leigh G. Griffiths, Rafael Shimkunas, Zana Coulibaly, Zhong Jian, Kristin N. Grimsrud, Claus S. Sondergaard, Kenneth S. Ginsburg, Nipavan Chiamvimonvat, Luiz Belardinelli, András Varró, Julius G. Papp, Piero Pollesello, Jouko Levijoki, Leighton T. Izu, W. Douglas Boyd, Tamás Bányász, Donald M. Bers, Ye Chen-Izu
Dátum:2018
ISSN:0027-8424 1091-6490
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Proceedings Of The National Academy Of Sciences Of The United States Of America. - 115 : 13 (2018), p. E3036-E3044. -
További szerzők:Bossuyt, Julie Griffiths, Leigh G. Shimkunas, Rafael Coulibaly, Zana Jian, Zhong Grimsrud, Kristin N. Sondergaard, Claus S. Ginsburg, Kenneth S. Chiamvimonvat, Nipavan Belardinelli, Luiz Varró András (1954-) (farmakológus, klinikai farmakológus) Papp Gy. Julius (Szeged) Pollesello, Piero Levijoki, Jouko Izu, Leighton T. Boyd, W. Douglas Bányász Tamás (1960-) (élettanász) Bers, Donald M. Chen-Izu, Ye
Internet cím:DOI
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001-es BibID:BIBFORM079019
035-os BibID:(absztrakt azonosító)3076-Pos Board B284 (WoS)000430563300097
Első szerző:Jian, Zhong
Cím:Mechanical Load Effects on Cardiomyocyte Action Potential, Cacium Transient, and Contraction Revealed by using a Novel Patch-Clamp-in-Gel Technology / Zhong Jian, Yi-je Chen, Bence Hegyi, Tamas Banyasz, Zana Coulibaly, Rafael Shimkunas, Nipavan Chiamvimonvat, Kit S. Lam, Leighton T. Izu, Ye Chen-Izu
Dátum:2018
ISSN:0006-3495
Megjegyzések:Background: In every heartbeat, cardiomyocytes are under mechanical load when the heart pumps blood against peripheral resistance. However, because previous patch-clamp experiments are mostly done using load-free cells, the mechanical load effects on cells were precluded. Method: We have developed an innovative Patch-Clamp-in-Gel technique which enables us to patch-clamp cardiomyocytes while they are embedded in a viscoelastic 3-D hydrogel that imposes a controlled mechanical load on cells. Results: We performed Patch-Clamp-in-Gel experiments using mouse ventricular myocytes and discovered the following major results. (1) Compared to load-free cells, the myocytes in-gel under mechanical load show prolonged action potential (AP), early afterdepolarization (EAD), delayed afterdepolarization (DAD) and triggered AP, suggesting that mechanical load significantly increases arrhythmogenic AP activities. (2) Simultaneous triple-signal recordings of AP, Ca2+, and contraction further reveal that load-induced EAD occurs independently while load-induced DAD occurs in conjunction with spontaneous Ca2+ tide and contraction. (3) The above load-induced changes can be reversed by specific inhibition of the nitric oxide synthase 1 (NOS1 or nNOS), demonstrating a critical role of nNOS in mediating the mechanotransduction pathway. Conclusion: Our newly developed Patch-Clamp-in-Gel technology provides a powerful tool to control mechanical load on single cells for studying mechanotransduction effects on ion channels, action potential, Ca2+ signaling, and myocyte contraction. We found that mechanical load significantly affects the three dynamic systems - electrical, Ca2+ signaling, and contractile systems - that govern cardiac function and arrhythmogenic activities, and nNOS-NO signaling mediates the mechanotransduction in cardiomyocytes.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idézhető absztrakt
folyóiratcikk
Megjelenés:Biophysical Journal. - 114 : 3 (2018), p. 620a. -
További szerzők:Chen, Yi-Je Hegyi Bence (1987-) (élettanász) Bányász Tamás (1960-) (élettanász) Coulibaly, Zana Shimkunas, Rafael Chiamvimonvat, Nipavan Lam, Kit S. Izu, Leighton T. Chen-Izu, Ye
Internet cím:Szerző által megadott URL
DOI
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