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001-es BibID:	BIBFORM041252
035-os BibID:	PMID:22654828
Első szerző:	Hrabovszky Erik
Cím:	Sexual dimorphism of kisspeptin and neurokinin B immunoreactive neurons in the infundibular nucleus of aged men and women / Erik Hrabovszky, Csilla S. Molnár, Máté T. Sipos, Barbara Vida, Philippe Ciofi, Beáta A. Borsay, László Sarkadi, László Herczeg, Stephen R. Bloom, Mohammad A. Ghatei, Waljit S. Dhillo, Imre Kalló, Zsolt Liposits
Dátum:	2011
Megjegyzések:	The secretory output of gonadotropin-releasing hormone (GnRH) neurons is critically influenced by peptidergic neurons synthesizing kisspeptins (KP) and neurokinin B (NKB) in the hypothalamic infundibular nucleus (Inf). These cells mediate negative feedback effects of sex steroids on the reproductive axis. While negative feedback is lost in postmenopausal women, it is partly preserved by the sustained testosterone secretion in aged men. We hypothesized that the different reproductive physiology of aged men and women is reflected in morphological differences of KP and NKB neurons. This sexual dimorphism was studied with immunohistochemistry in hypothalamic sections of aged human male (?50?years) and female (>55?years) subjects. KP and NKB cell bodies of the Inf were larger in females. The number of KP cell bodies, the density of KP fibers, and the incidence of their contacts on GnRH neurons were much higher in aged women compared with men. The number of NKB cell bodies was only slightly higher in women and there was no sexual dimorphism in the regional density of NKB fibers and the incidence of their appositions onto GnRH cells. The incidences of NKB cell bodies, fibers, and appositions onto GnRH neurons exceeded several-fold those of KP-IR elements in men. More NKB than KP inputs to GnRH cells were also present in women. Immunofluorescent studies identified only partial overlap between KP and NKB axons. KP and NKB were colocalized in higher percentages of afferents to GnRH neurons in women compared with men. Most of these sex differences might be explained with the lack of estrogen negative feedback in aged women, whereas testosterone can continue to suppress KP, and to a lesser extent, NKB synthesis in men. Overall, sex differences in reproductive physiology of aged humans were reflected in the dramatic sexual dimorphism of the KP system, with significantly higher incidences of KP-IR neurons, fibers and inputs to GnRH neurons in aged females vs. males.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban külföldön készült közlemény
Megjelenés:	Frontiers in endocrinology [electronic resource]. - 2 (2011), p. 80. -
További szerzők:	Molnár Csilla S. (Budapest) Sipos Máté T. Vida Barbara Ciofi, Philippe Borsay Beáta Á. (1982-) (igazságügyi orvosszakértő) Sarkadi László Herczeg László (1954-) (igazságügyi orvosszakértő) Bloom, Stephen R. Ghatei, Mohammad A. Dhillo, Waljit S. Kalló Imre Liposits Zsolt
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001-es BibID:	BIBFORM041254
035-os BibID:	PMID:22510271
Első szerző:	Hrabovszky Erik
Cím:	Glutamatergic and GABAergic innervation of human gonadotropin-releasing hormone-I neurons / Erik Hrabovszky, Csilla S. Molnár, Robert Nagy, Barbara Vida, Beáta Á. Borsay, Kálmán Rácz, László Herczeg, Masahiko Watanabe, Imre Kalló, Zsolt Liposits
Dátum:	2012
ISSN:	0013-7227
Megjegyzések:	Amino acid (aa) neurotransmitters in synaptic afferents to hypothalamic GnRH-I neurons are critically involved in the neuroendocrine control of reproduction. Although in rodents the major aa neurotransmitter in these afferents is ?-aminobutyric acid (GABA), glutamatergic axons also innervate GnRH neurons directly. Our aim with the present study was to address the relative contribution of GABAergic and glutamatergic axons to the afferent control of human GnRH neurons. Formalin-fixed hypothalamic samples were obtained from adult male individuals (n = 8) at autopsies, and their coronal sections processed for dual-label immunohistochemical studies. GABAergic axons were labeled with vesicular inhibitory aa transporter antibodies, whereas glutamatergic axons were detected with antisera against the major vesicular glutamate transporter (VGLUT) isoforms, VGLUT1 and VGLUT2. The relative incidences of GABAergic and glutamatergic axonal appositions to GnRH-immunoreactive neurons were compared quantitatively in two regions, the infundibular and paraventricular nuclei. Results showed that GABAergic axons established the most frequently encountered type of axo-somatic apposition. Glutamatergic contacts occurred in significantly lower numbers, with similar contributions by their VGLUT1 and VGLUT2 subclasses. The innervation pattern was different on GnRH dendrites where the combined incidence of glutamatergic (VGLUT1 + VGLUT2) contacts slightly exceeded that of the GABAergic appositions. We conclude that GABA represents the major aa neurotransmitter in axo-somatic afferents to human GnRH neurons, whereas glutamatergic inputs occur somewhat more frequently than GABAergic inputs on GnRH dendrites. Unlike in rats, the GnRH system of the human receives innervation from the VGLUT1, in addition to the VGLUT2, subclass of glutamatergic neurons.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban egyetemen (Magyarországon) készült közlemény
Megjelenés:	Endocrinology. - 153 : 6 (2012), p. 2766-2776. -
További szerzők:	Molnár Csilla S. (Budapest) Nagy Róbert (Budapest) Vida Barbara Borsay Beáta Á. (1982-) (igazságügyi orvosszakértő) Rácz Kálmán (1981-) (igazságügyi rezidens) Watanabe, Masahiko Kalló Imre Liposits Zsolt Herczeg László (1954-) (igazságügyi orvosszakértő)
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001-es BibID:	BIBFORM041250
Első szerző:	Kalló Imre
Cím:	A Novel Pathway Regulates Thyroid Hormone Availability in Rat and Human Hypothalamic Neurosecretory Neurons / Imre Kalló, Petra Mohácsik, Barbara Vida, Anikó Zeöld, Zsuzsanna Bardóczi, Ann Marie Zavacki, Erzsébet Farkas, Andrea Kádár, Erik Hrabovszky, Rafael Arrojo e Drigo, Liping Dong, László Barna, Miklós Palkovits, Beáta A. Borsay, László Herczeg, Ronald M. Lechan, Antonio C. Bianco, Zsolt Liposits, Csaba Fekete, Balázs Gereben
Dátum:	2012
ISSN:	1932-6203
Megjegyzések:	Hypothalamic neurosecretory systems are fundamental regulatory circuits influenced by thyroid hormone. Monocarboxylate-transporter-8 (MCT8)-mediated uptake of thyroid hormone followed by type 3 deiodinase (D3)-catalyzed inactivation represent limiting regulatory factors of neuronal T3 availability. In the present study we addressed the localization and subcellular distribution of D3 and MCT8 in neurosecretory neurons and addressed D3 function in their axons. Intense D3-immunoreactivity was observed in axon varicosities in the external zone of the rat median eminence and the neurohaemal zone of the human infundibulum containing axon terminals of hypophysiotropic parvocellular neurons. Immuno-electronmicroscopy localized D3 to dense-core vesicles in hypophysiotropic axon varicosities. N-STORM-superresolution-microscopy detected the active center containing C-terminus of D3 at the outer surface of these organelles. Double-labeling immunofluorescent confocal microscopy revealed that D3 is present in the majority of GnRH, CRH and GHRH axons but only in a minority of TRH axons, while absent from somatostatin-containing neurons. Bimolecular-Fluorescence-Complementation identified D3 homodimers, a prerequisite for D3 activity, in processes of GT1-7 cells. Furthermore, T3-inducible D3 catalytic activity was detected in the rat median eminence. Triple-labeling immunofluorescence and immuno-electronmicroscopy revealed the presence of MCT8 on the surface of the vast majority of all types of hypophysiotropic terminals. The presence of MCT8 was also demonstrated on the axon terminals in the neurohaemal zone of the human infundibulum. The unexpected role of hypophysiotropic axons in fine-tuned regulation of T3 availability in these cells via MCT8-mediated transport and D3-catalyzed inactivation may represent a novel regulatory core mechanism for metabolism, growth, stress and reproduction in rodents and humans.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban külföldön készült közlemény
Megjelenés:	PLoS One (electronic resource). - 7 : 6 (2012), p. e37860. -
További szerzők:	Mohácsik Petra Vida Barbara Zeöld Anikó Bardóczi Zsuzsanna Zavacki Ann Marie Farkas Erzsébet Kádár Andrea Hrabovszky Erik Arrojo e Drigo, Rafael Dong, Liping Barna László Palkovits Miklós Borsay Beáta Á. (1982-) (igazságügyi orvosszakértő) Lechan, Ronald M. Bianco, Antonio C. Liposits Zsolt Fekete Csaba Gereben Balázs Herczeg László (1954-) (igazságügyi orvosszakértő)
Pályázati támogatás:	K81226 OTKA K81845 OTKA K69127 OTKA
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