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001-es BibID:BIBFORM051720
035-os BibID:PMID:23775590
Első szerző:Kristóf Endre (általános orvos)
Cím:Novel role of ICAM3 and LFA-1 in the clearance of apoptotic neutrophils by human macrophages / Endre Kristóf, Gábor Zahuczky, Klára Katona, Zoltán Doró, Éva Nagy, László Fésüs
Dátum:2013
ISSN:1360-8185
Megjegyzések:Apoptotic cells express eat-me signals which are recognized by several receptors mainly on professional phagocytes of the mononuclear phagocyte system. This "engulfment synapse" can define a safe and effective clearance of apoptotic cells in order to maintain tissue homeostasis in the entire body. We show that the expression of four genes related to apoptotic cell clearance is strongly up-regulated in human macrophages 30 min after administration of apoptotic neutrophils. Out of these the significant role of the up-regulated intercellular adhesion molecule 3 (ICAM3) in phagocytosis of apoptotic neutrophils could be demonstrated in macrophages by gene silencing as well as treatment with blocking antibodies. Blocking ICAM3 on the surface of apoptotic neutrophils also resulted in their decreased uptake which confirmed its role as an eat-me signal expressed by apoptotic cells. In macrophages but not in neutrophils silencing and blocking integrin alphaL and beta2 components of lymphocyte function-associated antigen 1 (LFA-1), which can strongly bind ICAM3, resulted in a decreased phagocytosis of apoptotic cells indicating its possible role to recognize ICAM3 on the surface of apoptotic neutrophils. Finally, we report that engulfing portals formed in macrophages during phagocytosis are characterized by accumulation of ICAM3, integrin alphaL and beta2 which show co-localization on the surface of phagocytes. Furthermore, their simultaneous knock-down in macrophages resulted in a marked deficiency in phagocytosis and a slight decrease in the anti-inflammatory effect of apoptotic neutrophils. We propose that ICAM3 and LFA-1 act as recognition receptors in the phagocytosis portals of macrophages for engulfment of apoptotic neutrophils.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Molekuláris Medicina
Doktori iskola
Megjelenés:Apoptosis. - 18 : 10 (2013), p. 1235-1251. -
További szerzők:Zahuczky Gábor (1975-) (molekuláris biológus, biokémikus, vegyész) Katona Klára (1976-) Doró Zoltán (1981-) (biotechnológus) Nagy Éva (1980-) (molekuláris biológus) Fésüs László (1947-) (orvos biokémikus)
Pályázati támogatás:NK 105046
OTKA
K 61868
OTKA
TÁMOP-4.2.1/B-09/1/KONV-2010-0007
TÁMOP
Az apoptózis molekuláris mechanizmusa
TÁMOP-4.2.2/B-10/1-2010-0024
TÁMOP
Molekuláris Sejt- és Immunbiológiai Doktori Iskola
TRANSCOM IAPP251506
FP7
TRANSPATH ITN 289964
FP7
NKTH NTP Schizo08
Egyéb
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