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001-es BibID:	BIBFORM079713
035-os BibID:	(WoS)000486972404107
Első szerző:	Arianti, Rini (biokémikus)
Cím:	Identification of unique molecular signature ofbrowning in human primary adipocytes fromdeep and subcutaneous neck fat / Arianti Rini, Shaw Abhirup, Vámos Attila, Bartáné Tóth Beáta, Győry Ferenc, Póliska Szilárd, Kristóf Endre Károly, Fésüs László
Dátum:	2019
ISSN:	2211-5463
Megjegyzések:	There are two types of thermogenic adipocytes, classical brown and beige (BAT) which are UCP1-positive dissipating energy as heat. BAT markers have been well studied in rodents but detailed molecular studies are still lacking in humans where BAT is interspersed at several sites and may serve as a target of anti-obesity therapies. Our study aims to identify the unique signature of browning in human primary adipocytes from the different anatomical location by analyzing global gene expression patterns. Preadipocytes were obtained from subcutaneous (SC) and deep neck (DN) and differentiated to white and brown adipocytes. We analyzed differential gene expressions by total RNA sequencing, molecular pathways by KEGG Mapper, genetic constraint by ExAC and verified several genes of interest associated with adipocytes browning. We identified 37 genes which are closely clustered to UCP1. Out of those 13 genes have been already described to play a role in thermogenesis (CIDEA, CKMT1A/B), while the roles of the others are still unclear (ANO5, FAM151a). Several pathways were represented, such as retinoic acid biosynthesis which was upregulated (CPT1, CYP261B), while extracellular matrix organization pathways were among the downregulated ones (COL, ITGF). Mitochondrial creatine kinases, CKMT1a/b, are reported to play role in UCP1-independent thermogenesis; UCP1 and CKMT1a were expressed higher in DN, as compared to SC adipocytes and this was verified by RT-qPCR. Several transporters were expressed higher in DN, such as transporter of amino acids (SLC7A10), glutamate (SLC25A18) and pyruvate (SLC16A7). Our data proves that progenitors from DN fat can be differentiated to browning adipocytes at a greater extent than SC ones. We have started to investigate revealed molecular elements not linked yet to browning by deleting, inhibiting or overexpressing them.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok poszter folyóiratcikk
Megjelenés:	FEBS Open Bio. - 9 : S1 (2019), p. 289-290. -
További szerzők:	Shaw, Abhirup (1992-) Vámos Attila (1991-) (gyógyszer-biotechnológus) Bartáné Tóth Beáta (1970-) (molekuláris biológus) Győry Ferenc (1969-) (kardiológus) Póliska Szilárd (1978-) (biológus) Kristóf Endre (1987-) (általános orvos) Fésüs László (1947-) (orvos biokémikus)
Pályázati támogatás:	GINOP-2.3.2-15-2016-00006 GINOP
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM078217
035-os BibID:	(Wos)000437674103108
Első szerző:	Klusóczki Ágnes (biotechnológus)
Cím:	SGBS preadipocyte cell line can serve for human beige type of thermogenic browning adipocyte differentiation / A. Klusóczki, E. Kristóf, P. Fischer-Posovszky, M. Wabitsch, Z. Bacsó, L. Fésüs
Dátum:	2018
ISSN:	2211-5463
Tárgyszavak:	Orvostudományok Elméleti orvostudományok poszter folyóiratcikk
Megjelenés:	FEBS Open Bio. - 8 : S1 (2018), p. 241-242. -
További szerzők:	Kristóf Endre (1987-) (általános orvos) Fischer-Posovszky Pamela Wabitsch, Martin Bacsó Zsolt (1963-) (biofizikus) Fésüs László (1947-) (orvos biokémikus)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM102713
Első szerző:	Shaw, Abhirup
Cím:	Irisin stimulates the release of CXCL1 via upregulation of NFkB pathway from human neck derived differentiating adipocytes / Shaw Abhirup, B. Tóth Beáta, Győry Ferenc, Fésüs László, Kristóf Endre
Dátum:	2022
ISSN:	2211-5463
Megjegyzések:	Obesity is at present a global epidemic. Brown and beige adipocytes function in utilizing excess fat to produce heat (thermogenesis), thereby counteracting obesity. Recent studies in rodents and humans indicated that these adipocytes also release cytokines, which may play a vital role in maintaining whole body energy homeostasis. Irisin is primarily released by myocytes during exercise and functions as a polypeptide regulator of beige adipocytes. We intended to characterize the effect of irisin on differentiating adipocytes derived from human subcutaneous neck (SC) and deep neck (DN) adipose tissue depots. Preadipocytes were isolated from SC and DN biopsies of the same donor, differentiated to adipocytes in the presence or absence of irisin. Global gene expression analysis was performed on nine independent donors. Irisin could not upregulate characteristic thermogenic genes, but upregulated genes related to several cytokines. Out of them,CXCL1 (the highest upregulated) was found to be released throughout the differentiation period, predominantly by SC and DN differentiated adipocytes. DN tissue biopsies showed a significant release of CXCL1 upon 24 hours irisin treatment. Geneexpression data indicated upregulation of the NFkB pathway upon irisin treatment, which was validated by an increase of p50 and decrease of IkBa protein level, respectively. Continuous blocking of the NFkB pathway by SN50 (cell permeable inhibitorof NFkB nuclear translocation) significantly reduced the release of CXCL1. The released CXCL1 exerted a positive effect on the adhesion capability of endothelial cells. Together, our findings demonstrate that irisin stimulates the release of a novel adipokine, CXCL1, via upregulation of NFkB pathway in human neck area derived differentiating adipocytes, which plays an important role in improving tissue vascularization (Previously published in:Shaw A et al. (2021) Front Cell Dev Biol 9:737872).
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idézhető absztrakt folyóiratcikk
Megjelenés:	FEBS Open Bio. - 12 : S1 (2022), p. 208. -
További szerzők:	Bartáné Tóth Beáta (1970-) (molekuláris biológus) Győry Ferenc (1964-) (sebész) Fésüs László (1947-) (orvos biokémikus) Kristóf Endre (1987-) (általános orvos)
Pályázati támogatás:	FK131424 OTKA GINOP-2.3.2-15-2016-00006 GINOP
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM095719
035-os BibID:	(WoS)000668898600016
Első szerző:	Shaw, Abhirup
Cím:	Mitophagy is downregulated upon thermogenic stimulus in human beige adipocytes / Shaw Abhirup, Szatmári-Tóth Mária, Csomós István, Mocsár Gábor, Balajthy Zoltán, Győry Ferenc, Kristóf Endre Károly, Fésüs László
Dátum:	2021
ISSN:	2211-5463
Megjegyzések:	Adipocytes are classi?ed into white, brown and beige. Brown and beige adipocytes are important targets to combat obesity, as they are capable to dissipate energy in the form of heat, while the white adipocytes are primarily for energy storage. UCP1, an inner mitochondrial membrane protein mediates thermogenesis by uncoupling the mitochondrial respiratory chain from ATP synthesis. Hence, mitochondria are important for the thermogenic and metabolic functions of adipocytes. UCP1+ mitochondria in human adipocytes are mostly fragmented (Pisani et al, 2017). Mitophagy plays a vital role in beige to white adipocyte transition in mouse (Altshuler-Keylin et al, 2016). We intend to characterize the role of mitophagy in the thermogenic activation of primary human abdominal subcutaneous adipocytes and SGBS cells. Isolated preadipocytes were differentiated into white and browning adipocytes, which were treated with dibutyryl-cAMP (6, 10 and 14 hours). Genes related to parkin-dependent and independent mitophagy were downregulated upon thermogenic stimulus; the parkin dependent ones being the most downregulated. The lipidated form of LC3, LC3-II is recruited on the outer membrane of the autophagosome which indicates ongoing autophagy. TOM20 is an outer mitochondrial membrane protein and marks the mitochondria. LC3 and TOM20 immunostaining were performed, followed by quanti?cation of LC3 punctae,which was high in untreated control adipocytes but decreased signi?cantly upon thermogenic stimulus, suggesting repressed autophagy/mitophagy. Colocalization of TOM20 and LC3 can indicate mitophagy. Decreased colocalization was observed upon thermogenic stimulus, which further proved repressed mitophagy.TOM20 quanti?cation showed increased number of fragmented mitochondria upon thermogenic stimulus suggesting prompt inhibition of mitophagy, thereby protecting many fragmented mitochondria from degradation and boosting thermogenesis.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idézhető absztrakt folyóiratcikk
Megjelenés:	FEBS Open Bio. - 11 : S1 (2021), p. 9. -
További szerzők:	Szatmári-Tóth Mária (1987-) (molekuláris biológus) Csomós István (1983-) (molekuláris biológus) Mocsár Gábor (1981-) (biofizikus) Balajthy Zoltán (1957-) (biokémikus, sejtbiológus) Győry Ferenc (1964-) (sebész) Kristóf Endre (1987-) (általános orvos) Fésüs László (1947-) (orvos biokémikus)
Pályázati támogatás:	GINOP-2.3.2-15-2016-00006 GINOP
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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