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001-es BibID:	BIBFORM061542
Első szerző:	Razeghi, Peter
Cím:	Downregulation of metabolic gene expression in failing human heart before and after mechanical unloading / Razeghi P., Young M. E., Ying J., Depre C., Uray I. P., Kolesar J., Shipley G. L., Moravec C. S., Davies P. J. A., Frazier O. H., Taegtmeyer H.
Dátum:	2002
ISSN:	0008-6312
Megjegyzések:	Background: We have previously shown that several metabolic genes are downregulated in the failing human heart. We now tested the hypothesis that mechanical unloading might reverse this process. Methods: Clinical data and myocardial tissue were collected from 14 failing hearts paired for the time of implantation and explantation of a left ventricular assist device (LVAD) and compared to 10 non-failing hearts. Transcript levels for key regulators of energy metabolism and for atrial natriuretic factor (ANF) were measured by real-time quantitative RT-PCR. Results: The expression of the glucose transporter 1 and 4 (GLUT1, GLUT4), muscle carnitine palmitoyl transferase-1 (mCPT-1), and uncoupling protein 3 (UCP3) were downregulated by up to 80% in the failing heart. Although LVAD treatment improved clinical markers of heart failure (decrease of left ventricular diastolic dimension and normalization of serum sodium), only UCP3 expression reversed to non-failing transcript levels following mechanical unloading. Conclusions: LVAD treatment only partially reverses depressed metabolic gene expression in the failing human heart. Reversal of depressed UCP3 expression may be an important mechanism for reducing the formation of oxygen-derived free radicals. Further studies are necessary to define the effects of mechanical unloading on post-transcriptional mechanisms.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Cardiology. - 97 : 4 (2002), p. 203-209. -
További szerzők:	Young, Martin E. Ying, Jun Depre, Christophe Uray Iván Péter (1970-) (kutatóorvos) Kolesar, June Shipley, Gregory L. Moravec, Christine S. Davies, Peter J. A. Frazier, O. Howard Taegtmeyer, Heinrich
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