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1.

001-es BibID:BIBFORM060508
Első szerző:Albert Réka
Cím:Triamcinolone regulated apopto-phagocytic gene expression patterns in the clearance of dying retinal pigment epithelial cells. A key role of Mertk in the enhanced phagocytosis / Réka Albert, Endre Kristóf, Gábor Zahuczky, Mária Szatmári-Tóth, Zoltán Veréb, Brigitta Oláh, Morten C. Moe, Andrea Facskó, László Fésüs, Goran Petrovski
Dátum:2015
ISSN:0304-4165
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Biochimica et Biophysica Acta (BBA). General Subjects. - 1850 : 2 (2015), p. 435-446. -
További szerzők:Kristóf Endre (1987-) (általános orvos) Zahuczky Gábor (1975-) (molekuláris biológus, biokémikus, vegyész) Szatmári-Tóth Mária (1987-) (molekuláris biológus) Veréb Zoltán (1980-) (immunológus, mikrobiológus, molekuláris biológus) Oláh Brigitta Moe, Morten C. Facskó Andrea (1953-) (szemész) Fésüs László (1947-) (orvos biokémikus) Petrovski, Goran (1975-) (orvos)
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2.

001-es BibID:BIBFORM043609
Első szerző:Albert Réka
Cím:Cultivation and characterization of cornea limbal epithelial stem cells on lens capsule in animal material-free medium / Réka Albert, Zoltán Veréb, Krisztián Csomós, Morten C. Moe, Erik O. Johnsen, Ole Kristoffer Olstad, Bjørn Nicolaissen, Éva Rajnavölgyi, László Fésüs, András Berta, Goran Petrovski
Dátum:2012
ISSN:1932-6203
Megjegyzések:A simple, reproducible, animal-material free method for cultivating and characterizing cornea limbal epithelial stem cells (LESCs) on human lens capsule (LC) was developed for future clinical transplantation. The limbal tissue explants (2 ? 2 ? 0.25 mm) were harvested from 77 cadavers and expanded ex vivo on either cell culture plates or LC in medium containing human serum as the only growth supplement. Cell outgrowth at the edge of the explants was observed within 24 hours of cultivation and achieved viable outgrowth (>97% viability as measured by MTT assay and flow cytometry) within two weeks. The outgrowing cells were examined by genome-wide microarray including markers of stemness (p63?, ABCG2, CK19, Vimentin and Integrin ?9), proliferation (Ki-67), limbal epithelial cells (CK 8/18 and 14) and differentiated cornea epithelial cells (CK 3 and 12). Immunostaining revealed the non-hematopoietic, -endothelial and -mesenchymal stem cell phenotype of the LESCs and the localization of specific markers in situ. Cell adhesion molecules, integrins and lectin-based surface carbohydrate profiling showed a specific pattern on these cells, while colony-formation assay confirmed their clonal potency. The LESCs expressed a specific surface marker fingerprint (CD117/c-kit, CXCR4, CD144/VE-Cadherin, CD146/MCAM, CD166/ALCAM, and surface carbohydrates: WGA, ConA, RCA, PNA and AIL) which can be used for better localization of the limbal stem cell niche. In summary, we report a novel method combining the use of a medium with human serum as the only growth supplement with LC for cultivating, characterizing and expanding cornea LESCs from cadavers or alternatively from autologous donors for possible treatment of LESC deficiency.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Limbal epithelial stem cell
egyetemen (Magyarországon) készült közlemény
Megjelenés:PLoS One. - 7 : 10 (2012), p. e47187. -
További szerzők:Veréb Zoltán (1980-) (immunológus, mikrobiológus, molekuláris biológus) Csomós Krisztián (1981-) (molekuláris biológus) Moe, Morten C. Johnsen, Erik O. Olstad, Ole Kristoffer Nicolaissen, Bjorn Rajnavölgyi Éva (1950-) (immunológus) Fésüs László (1947-) (orvos biokémikus) Berta András (1955-) (szemész, gyermekszemész) Petrovski, Goran (1975-) (orvos)
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3.

001-es BibID:BIBFORM049103
Első szerző:Johnsen, Erik O.
Cím:Reply to comment on "Activation of neural progenitor cells in human eyes with proliferative vitreoretinopathy" by Johnsen et al. [Exp. Eye Res. 98 (2012) 28-36] / Erik O. Johnsen, Réka Albert, Bjørn Nicolaissen, Goran Petrovski, Morten C. Moe
Dátum:2012
ISSN:0014-4835
Tárgyszavak:Orvostudományok Elméleti orvostudományok szerkesztői levél
neural
stem cell
progenitor cell
retina
ciliary body epithelium
proliferative vitreoretinopathy
Megjelenés:Experimental Eye Research. - 105 (2012), p. 81-82. -
További szerzők:Albert Réka (1986-) Nicolaissen, Bjorn Petrovski, Goran (1975-) (orvos) Moe, Morten C.
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4.

001-es BibID:BIBFORM037065
Első szerző:Johnsen, Erik O.
Cím:Activation of neural progenitor cells in human eyes with proliferative vitreoretinopathy / Johnsen E. O., Froen R. C., Albert R., Omdal B. K., Sarang Z., Berta A., Nicolaissen B., Petrovski G., Moe M. C.
Dátum:2012
ISSN:0014-4835
Megjegyzések:In addition to the ability for self-renewal and functional differentiation, neural stem/progenitor cells (NSCs) can respond to CNS injuries by targeted migration. In lower vertebrates, retinal injury is known to activate NSCs in the ciliary marginal zone (CMZ). Cells expressing markers of NSCs are also present in the ciliary body epithelium (CE) and in Müller glia in the peripheral retina (PR) of the adult human eye. However, these cells seem to be quiescent in the adult human eye and recent reports have shown that CE cells have limited properties of NSCs. In order to further clarify whether NSCs exist in the adult human eye, we tested whether NSC-like cells could be activated in eyes with proliferative vitreoretinopathy (PVR). The PR and CE were studied for NSC-associated markers in human enucleated control eyes and eyes with confirmed PVR, as well as in a mouse model of PVR. Furthermore, cells isolated from vitreous samples obtained during vitrectomies for retinal detachment were directly fixed or cultured in a stem cell-promoting medium and compared to cells cultured from the post-mortem retina and CE. In situ characterization of the normal eyes revealed robust expression of markers present in NSCs (Nestin, Sox2, Pax6) only around peripheral cysts of the proximal pars plana region and the PR, the latter population also staining for the glial marker GFAP. Although there were higher numbers of dividing cells in the CE of PVR eyes than in controls, we did not detect NSC-associated markers in the CE except around the proximal pars plana cysts. In the mice PVR eyes, Nestin activation was also found in the CE. In human PVR eyes, proliferation of both non-glial and glial cells co-staining NSC-associated markers was evident around the ora serrata region. Spheres formed in 7/10 vitreous samples from patients with PVR compared to 2/15 samples from patients with no known PVR, and expressed glial - and NSC-associated markers both after direct fixation and repetitive passages. In conclusion, the adult human eye may harbor two different populations of neuroepithelial stem/progenitor cells; a non-glial population located in the proximal pars plana around peripheral cysts in addition to a population with Müller glia characteristics. Yet, we only found that the glial population was able to respond to retinal injury by targeted migration into the vitreous.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Experimental Eye Research. - 98 (2012), p. 28-36. -
További szerzők:Froen, Rebecca C. Albert Réka (1986-) Omdal, Bente K. Sarang Zsolt (1976-) (mikrobiológus) Berta András (1955-) (szemész, gyermekszemész) Nicolaissen, Bjorn Petrovski, Goran (1975-) (orvos) Moe, Morten C.
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5.

001-es BibID:BIBFORM062340
Első szerző:Petrovski, Goran (orvos)
Cím:Herpes simplex virus types 1 and 2 modulate autophagy in SIRC corneal cells / Goran Petrovski, Kata Pásztor, László Orosz, Réka Albert, Edina Mencel, Morten C. Moe, Kai Kaarniranta, Andrea Facskó, Klára Megyeri
Dátum:2014
ISSN:0250-5991
Megjegyzések:Autophagy and apoptosis function as important early cellular defense mechanisms in infections and other diseases. The outcome of an infection is determined by a complex interplay between the pathogenic microorganism and these intracellular pathways. To better understand the cytopathogenicity of Herpes simplex virus types 1 and 2 (HSV-1 and - 2), we studied the effect of these viruses on the autophagic and apoptotic processes in the SIRC corneal cell line. Infection with the KOS strain of HSV-1 and a wild-type strain of HSV-2 enhanced autophagosome formation, triggered cytoplasmic acidification, increased LC3B lipidation and elevated the ratio of apoptotic cells. The autophagy inhibitor bafilomycin A1 triggered a significant increase in the apoptotic responses of HSV-1 and HSV-2-infected cells. Thus, both HSV types affect autophagy and apoptosis in a coordinated fashion, and autophagy plays cytoprotective role in HSV-infected cells via antagonizing apoptosis. Together these data implicate autophagy in the pathogenic mechanism of herpetic keratitis.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Apoptosis
autophagy
corneal cells
Herpes simplex virus
keratitis
Megjelenés:Journal Of Biosciences. - 39 : 4 (2014), p. 683-692. -
További szerzők:Pásztor Kata Orosz László (Szeged) Albert Réka (1986-) Mencel Edina Moe, Morten C. Kaarniranta, Kai (1972-) (szemész szakorvos) Facskó Andrea (1953-) (szemész) Megyeri Klára
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6.

001-es BibID:BIBFORM049105
Első szerző:Petrovski, Goran (orvos)
Cím:Autophagy in the eye : a double-edged sword / Goran Petrovski, Réka Albert, Kai Kaarniranta, Morten C. Moe, László Fésüs, András Berta, Dipak K. Das
Dátum:2012
Megjegyzések:Autophagy, a cytoplasmic recycling process active from early development throughout lifetime and aging has turned from largely a morphological phenomenon to a well described molecular mechanism in health and disease. The eye as an organ in which an interplay of three germ layers is responsible for achieving complex visual response, is the place where battles for cellular life or death takes place continuously ? an organ in which the highest dead cell turnover occurs in the human body. The role of autophagy in the different segments of the eye remains not so well understood or described, making it difficult to classify autophagy as a protective or disease causing process. This chaptersummarizes the current knowledge about the role of autophagy in eye health and disease as well as the present drug targets applying autophagy as a protective mechanism against ophthalmic neurodegeneration and aging.
ISBN:978 1 61942 266 7
Tárgyszavak:Orvostudományok Elméleti orvostudományok könyvfejezet
Autophagy
double-edged sword
Megjelenés:Autophagy: Principles, Regulation and Roles in Disease / szerk. Nikolai Gorbunov. - p. 157-180. -
További szerzők:Albert Réka (1986-) Kaarniranta, Kai (1972-) (szemész szakorvos) Moe, Morten C. Fésüs László (1947-) (orvos biokémikus) Berta András (1955-) (szemész, gyermekszemész) Das, Dipak Kumar
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7.

001-es BibID:BIBFORM064011
035-os BibID:(Cikkazonosító)26227 (WOS)000376370500001 (Scopus)84970028330
Első szerző:Veréb Zoltán (immunológus, mikrobiológus, molekuláris biológus)
Cím:Role of Human Corneal Stroma-Derived Mesenchymal-Like Stem Cells in Corneal Immunity and Wound Healing / Zoltán Veréb, Szilárd Póliska, Réka Albert, Ole Kristoffer Olstad, Anita Boratkó, Csilla Csortos, Morten C. Moe, Andrea Facskó, Goran Petrovski
Dátum:2016
ISSN:2045-2322
Tárgyszavak:Orvostudományok Egészségtudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Scientific Reports. - 6 : 26227 (2016), p. 1-17. -
További szerzők:Póliska Szilárd (1978-) (biológus) Albert Réka (1986-) Olstad, Ole Kristoffer Boratkó Anita (1985-) (biokémikus, molekuláris biológus) Csortos Csilla (1956-) (biokémikus) Moe, Morten C. Facskó Andrea (1953-) (szemész) Petrovski, Goran (1975-) (orvos)
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8.

001-es BibID:BIBFORM049100
Első szerző:Veréb Zoltán (immunológus, mikrobiológus, molekuláris biológus)
Cím:Comparison of upstream regulators in human ex vivo cultured cornea limbal epithelial stem cells and differentiated corneal epithelial cells / Zoltán Veréb, Réka Albert, Szilárd Póliska, Ole Kristoffer Olstad, Saeed Akhtar, Morten C. Moe, Goran Petrovski
Dátum:2013
ISSN:1471-2164
Megjegyzések:BACKGROUND:The surface of the human eye is covered by corneal epithelial cells (CECs) which regenerate from a small population of limbal epithelial stem cells (LESCs). Cell therapy with LESCs is a non-penetrating treatment for preventing blindness due to LESC deficiency or dysfunction. Our aim was to identify new putative molecular markers and upstream regulators in the LESCs and associated molecular pathways.RESULTS:Genome-wide microarray transcriptional profiling was used to compare LESCs to differentiated human CECs. Ingenuity-based pathway analysis was applied to identify upstream regulators and pathways specific to LESCs. ELISA and flow cytometry were used to measure secreted and surface expressed proteins, respectively. More than 2 fold increase and decrease in expression could be found in 1830 genes between the two cell types. A number of molecules functioning in cellular movement (381), proliferation (567), development (552), death and survival (520), and cell-to-cell signaling (290) were detected having top biological functions in LESCs and several of these were confirmed by flow cytometric surface protein analysis. Custom-selected gene groups related to stemness, differentiation, cell adhesion, cytokines and growth factors as well as angiogenesis could be analyzed. The results show that LESCs play a key role not only in epithelial differentiation and tissue repair, but also in controlling angiogenesis and extracellular matrix integrity. Some pro-inflammatory cytokines were found to be important in stemness-, differentiation- and angiogenesis-related biological functions: IL-6 and IL-8 participated in most of these biological pathways as validated by their secretion from LESC cultures.CONCLUSIONS:The gene and molecular pathways may provide a more specific understanding of the signaling molecules associated with LESCs, therefore, help better identify and use these cells in the treatment of ocular surface diseases.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Limbal epithelial stem cells
Corneal epithelial cells
Gene array
Upstream regulators
Cytokines
Cell adhesion
IL-6
IL-8
Angiogenesis
Megjelenés:BMC Genomics. - 14 : 1 (2013), p. [1-33]. -
További szerzők:Albert Réka (1986-) Póliska Szilárd (1978-) (biológus) Olstad, Ole Kristoffer Akhtar, Saeed (1949-) (molekuláris biológus) Moe, Morten C. Petrovski, Goran (1975-) (orvos)
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