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001-es BibID:	BIBFORM043609
Első szerző:	Albert Réka
Cím:	Cultivation and characterization of cornea limbal epithelial stem cells on lens capsule in animal material-free medium / Réka Albert, Zoltán Veréb, Krisztián Csomós, Morten C. Moe, Erik O. Johnsen, Ole Kristoffer Olstad, Bjørn Nicolaissen, Éva Rajnavölgyi, László Fésüs, András Berta, Goran Petrovski
Dátum:	2012
ISSN:	1932-6203
Megjegyzések:	A simple, reproducible, animal-material free method for cultivating and characterizing cornea limbal epithelial stem cells (LESCs) on human lens capsule (LC) was developed for future clinical transplantation. The limbal tissue explants (2 ? 2 ? 0.25 mm) were harvested from 77 cadavers and expanded ex vivo on either cell culture plates or LC in medium containing human serum as the only growth supplement. Cell outgrowth at the edge of the explants was observed within 24 hours of cultivation and achieved viable outgrowth (>97% viability as measured by MTT assay and flow cytometry) within two weeks. The outgrowing cells were examined by genome-wide microarray including markers of stemness (p63?, ABCG2, CK19, Vimentin and Integrin ?9), proliferation (Ki-67), limbal epithelial cells (CK 8/18 and 14) and differentiated cornea epithelial cells (CK 3 and 12). Immunostaining revealed the non-hematopoietic, -endothelial and -mesenchymal stem cell phenotype of the LESCs and the localization of specific markers in situ. Cell adhesion molecules, integrins and lectin-based surface carbohydrate profiling showed a specific pattern on these cells, while colony-formation assay confirmed their clonal potency. The LESCs expressed a specific surface marker fingerprint (CD117/c-kit, CXCR4, CD144/VE-Cadherin, CD146/MCAM, CD166/ALCAM, and surface carbohydrates: WGA, ConA, RCA, PNA and AIL) which can be used for better localization of the limbal stem cell niche. In summary, we report a novel method combining the use of a medium with human serum as the only growth supplement with LC for cultivating, characterizing and expanding cornea LESCs from cadavers or alternatively from autologous donors for possible treatment of LESC deficiency.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Limbal epithelial stem cell egyetemen (Magyarországon) készült közlemény
Megjelenés:	PLoS One. - 7 : 10 (2012), p. e47187. -
További szerzők:	Veréb Zoltán (1980-) (immunológus, mikrobiológus, molekuláris biológus) Csomós Krisztián (1981-) (molekuláris biológus) Moe, Morten C. Johnsen, Erik O. Olstad, Ole Kristoffer Nicolaissen, Bjorn Rajnavölgyi Éva (1950-) (immunológus) Fésüs László (1947-) (orvos biokémikus) Berta András (1955-) (szemész, gyermekszemész) Petrovski, Goran (1975-) (orvos)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM049103
Első szerző:	Johnsen, Erik O.
Cím:	Reply to comment on "Activation of neural progenitor cells in human eyes with proliferative vitreoretinopathy" by Johnsen et al. [Exp. Eye Res. 98 (2012) 28-36] / Erik O. Johnsen, Réka Albert, Bjørn Nicolaissen, Goran Petrovski, Morten C. Moe
Dátum:	2012
ISSN:	0014-4835
Tárgyszavak:	Orvostudományok Elméleti orvostudományok szerkesztői levél neural stem cell progenitor cell retina ciliary body epithelium proliferative vitreoretinopathy
Megjelenés:	Experimental Eye Research. - 105 (2012), p. 81-82. -
További szerzők:	Albert Réka (1986-) Nicolaissen, Bjorn Petrovski, Goran (1975-) (orvos) Moe, Morten C.
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001-es BibID:	BIBFORM037065
Első szerző:	Johnsen, Erik O.
Cím:	Activation of neural progenitor cells in human eyes with proliferative vitreoretinopathy / Johnsen E. O., Froen R. C., Albert R., Omdal B. K., Sarang Z., Berta A., Nicolaissen B., Petrovski G., Moe M. C.
Dátum:	2012
ISSN:	0014-4835
Megjegyzések:	In addition to the ability for self-renewal and functional differentiation, neural stem/progenitor cells (NSCs) can respond to CNS injuries by targeted migration. In lower vertebrates, retinal injury is known to activate NSCs in the ciliary marginal zone (CMZ). Cells expressing markers of NSCs are also present in the ciliary body epithelium (CE) and in Müller glia in the peripheral retina (PR) of the adult human eye. However, these cells seem to be quiescent in the adult human eye and recent reports have shown that CE cells have limited properties of NSCs. In order to further clarify whether NSCs exist in the adult human eye, we tested whether NSC-like cells could be activated in eyes with proliferative vitreoretinopathy (PVR). The PR and CE were studied for NSC-associated markers in human enucleated control eyes and eyes with confirmed PVR, as well as in a mouse model of PVR. Furthermore, cells isolated from vitreous samples obtained during vitrectomies for retinal detachment were directly fixed or cultured in a stem cell-promoting medium and compared to cells cultured from the post-mortem retina and CE. In situ characterization of the normal eyes revealed robust expression of markers present in NSCs (Nestin, Sox2, Pax6) only around peripheral cysts of the proximal pars plana region and the PR, the latter population also staining for the glial marker GFAP. Although there were higher numbers of dividing cells in the CE of PVR eyes than in controls, we did not detect NSC-associated markers in the CE except around the proximal pars plana cysts. In the mice PVR eyes, Nestin activation was also found in the CE. In human PVR eyes, proliferation of both non-glial and glial cells co-staining NSC-associated markers was evident around the ora serrata region. Spheres formed in 7/10 vitreous samples from patients with PVR compared to 2/15 samples from patients with no known PVR, and expressed glial - and NSC-associated markers both after direct fixation and repetitive passages. In conclusion, the adult human eye may harbor two different populations of neuroepithelial stem/progenitor cells; a non-glial population located in the proximal pars plana around peripheral cysts in addition to a population with Müller glia characteristics. Yet, we only found that the glial population was able to respond to retinal injury by targeted migration into the vitreous.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Experimental Eye Research. - 98 (2012), p. 28-36. -
További szerzők:	Froen, Rebecca C. Albert Réka (1986-) Omdal, Bente K. Sarang Zsolt (1976-) (mikrobiológus) Berta András (1955-) (szemész, gyermekszemész) Nicolaissen, Bjorn Petrovski, Goran (1975-) (orvos) Moe, Morten C.
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