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001-es BibID:	BIBFORM073605
Első szerző:	Zolnai A.
Cím:	Comparison of 3H-thymidine incorporation and CellTiter 96 aqueous colorimetric assays in cell proliferation of bovine mononuclear cells / Zolnai A., Tóth E. B., Wilson R. A., Frenyó V. L.
Dátum:	1998
ISSN:	0236-6290
Megjegyzések:	A rapid colorimetric non-radioactive assay for the determination of bovine mitogen-induced lymphocyte proliferation in cell culture was evaluated using a novel tetrazolium compound (MTS) and an electron coupling reagent (PMS) provided in the CellTiter 96? kit (Promega). The results of the new method were compared with those of the 3H-thymidine incorporation assay using parallels obtained from the same lymphocyte population. The concentrations used in the cell suspension of primary cultured lymphocytes resulted in a significant signal/background ratio when cells were prepared from peripheral blood, spleen or mesenteric lymph nodes. The same concentrations of thymocytes resulted in a weak signal even for the highest concentrations of mitogen. A good correlation was demonstrated between the results of the two methods. The non-radioactive method performed well in assays in bovine mononuclear cells derived from prolactin-treated or -untreated calves, showing a 50% lower responsiveness to mitogenic stimulation in prolactin-deprived animals.
Tárgyszavak:	Orvostudományok Gyógyszerészeti tudományok idegen nyelvű folyóiratközlemény hazai lapban
Megjelenés:	Acta Veterinaria Hungarica 46 : 2 (1998), p. 191-197. -
További szerzők:	Tóth E. Béla (1960-) (orvos) Wilson, R. A. Frenyó V. L.
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