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001-es BibID:	BIBFORM058347
Első szerző:	Guttmann Miklós
Cím:	Comparative glycoprofiling of HIV gp120 immunogens by capillary electrophoresis and MALDI mass spectrometry / Miklós Guttman, Csaba Váradi, Kelly K. Lee, András Guttman
Dátum:	2015
ISSN:	0173-0835
Megjegyzések:	The Human Immunodeficiency Virus (HIV) envelope glycoprotein (Env) is the primary antigenic feature on the surface of the virus and is of key importance in HIV vaccinology. Vaccine trials with the gp120 subunit of Env are ongoing with the recent RV144 trial showing moderate efficacy. gp120 is densely covered with N-linked glycans that are thought to help evade the host's humoral immune response. To assess how the global glycosylation patterns vary between gp120 constructs, the glycan profiles of several gp120s were examined by capillary electrophoresis with laser induced fluorescence detection and MALDI-MS. The glycosylation profiles were found to be similar for chronic vs. transmitter/founder isolates and only varied moderately between gp120s from different clades. This study revealed that the addition of specific tags, such as the gD tag used in the RV144 trial, had significant effects on the overall glycosylation patterns. Such effects are likely to influence the immunogenicity of various Env immunogens and should be considered for future vaccine strategies, emphasizing the importance of the glycosylation analysis approach described in this paper.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban HIV glycosylation
Megjelenés:	Electrophoresis 36 : 11-12 (2015), p. 1305-1313. -
További szerzők:	Váradi Csaba (1988-) (molekuláris biológus) Lee, Kelly K. Guttman András (1954-) (vegyészmérnök)
Pályázati támogatás:	NIH F32-GM097805 Egyéb
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM077852
035-os BibID:	(PMID)29330871 (Scopus)85041568961 (WOS)000429417500010
Első szerző:	Szekrényes Ákos (vegyészmérnök)
Cím:	Multi-site N-Glycan mapping study 2 : UHPLC / Ákos Szekrényes, SungAe Suhr Park, Eoin Cosgrave, Aled Jones, Ted Haxo, Michael Kimzey, Shiva Pourkaveh, Zoltán Szabó, Zoran Sosic, Peng Feng, Preeti Sejwal, Kelsey Dent, David Michels, Gordon Freckleton, Jun Qian, Catherine Lancaster, Toni Duffy, Melissa Schwartz, Jiann-Kae Luo, Jonathan van Dyck, Pui-King Leung, Marcell Olajos, Ronald Kowle, Kai Gao, Wenbo Wang, Jo Wegstein, Samnang Tep, Apolka Domokos, Csaba Váradi, András Guttman
Dátum:	2018
ISSN:	0173-0835 1522-2683
Megjegyzések:	In the first part of this publication, the results from an international study evaluating theprecision (i.e., repeatability and reproducibility) of N-glycosylation analysis using capillaryelectrophoresis of APTS-labeled N-glycans were presented. The corresponding resultsfrom ultra-high performance liquid chromatography (UHPLC) with fluorescence detectionare presented here from 12 participating sites. All participants used the same lot of samples,reagents, and columns to perform the assays. Elution time, peak area and peak areapercent values were determined for all peaks 0.1% peak area, and statistical analysis wasperformed following ISO 5725-2 guideline principles. The results demonstrated adequatereproducibility, within any given site as well across all sites, indicating that standardUHPLC-based N-glycan analysis platforms are appropriate for general use
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk 2-AB Biotherapeutics Intercompany study N-glycans UHPLC
Megjelenés:	Electrophoresis. - 39 : 7 (2018), p. 998-1005. -
További szerzők:	Park, SungAe Suhr Cosgrave, Eoin Jones, Aled Haxo, Ted Kimzey, Michael Pourkaveh, Shiva Szabó Zoltán (orvos) Sosic, Zoran Feng, Peng Sejwal, Preeti Dent, Kelsey Michels, David Freckleton, Gordon Qian, Jun Lancaster, Catherine Duffy, Toni Schwartz, Melissa Luo, Jiann-Kae van Dyck, Jonathan Leung, Pui-King Olajos Marcell Kowle, Ronald Gao, Kai Wang, Wenbo Wegstein, Jo Tep, Samnang Domokos Apolka Váradi Csaba (1988-) (molekuláris biológus) Guttman András (1954-) (vegyészmérnök)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM058339
Első szerző:	Váradi Csaba (molekuláris biológus)
Cím:	Combination of IgG N-glycomics and corresponding transcriptomics data to identify anti-TNFα treatment responders in inflammatory diseases / Csaba Váradi, Zsolt Holló, Szilárd Póliska, László Nagy, Zoltán Szekanecz, Andrea Váncsa, Károly Palatka, András Guttman
Dátum:	2015
ISSN:	0173-0835
Megjegyzések:	Prediction of responsiveness in biological therapies is an important and challenging issue in different diseases. Analyzing glycosylation pattern changes of key serum glycoproteins is one of the possible avenues to follow disease remission. The aim of this study was to investigate the changes of serum IgG glycoforms in Crohn's disease (CD) and rheumatoid arthritis patients in response to antitumor necrosis factor alpha (anti-TNF-?) treatment. IgG was isolated from patient serum samples using Protein A affinity pull-down, followed by the release of N-glycans with peptide-N-glycosidase F. The released glycans were fluorescently tagged with 8-aminopyrene-1,3,6-trisulfonate and analyzed by CGE with laser-induced fluorescent detection. Significant alterations were detected between responders and nonresponders in both disease groups. In CD patients, disease-specific alteration was found in response to anti-TNF-? therapy, which was also confirmed by transcriptomics data analysis of the corresponding glycosyltransferases and glycosidases.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban glikozilacio
Megjelenés:	Electrophoresis. - 36 : 11-12 (2015), p. 1330-1335. -
További szerzők:	Holló Zsolt Póliska Szilárd (1978-) (biológus) Nagy László (1966-) (molekuláris sejtbiológus, biokémikus) Szekanecz Zoltán (1964-) (reumatológus, belgyógyász, immunológus) Váncsa Andrea (1972-) (orvos) Palatka Károly (1961-) (belgyógyász, gasztroenterológus) Guttman András (1954-) (vegyészmérnök)
Pályázati támogatás:	MTA-PE Translational Glycomics MTA
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM043657
Első szerző:	Váradi Csaba (molekuláris biológus)
Cím:	Analysis of Haptoglobin N-glycome Alterations in Inflammatory and Malignant Lung Diseases by Capillary Electrophoresis / Cs. Varadi, S. Mittermayr, A. Szekrenyes, J. Kadas, L. Takacs, I. Kurucz, A. Guttman
Dátum:	2013
ISSN:	0173-0835
Megjegyzések:	A capillary electrophoresis based method was introduced to compare the N-glycosylation profile ofhaptoglobin in normal and pathologic conditions. To assess the biomarker potential of glycosylationchanges in various lung diseases, haptoglobin was isolated from plasma samples of healthy, pneumonia,chronic obstructive pulmonary disease (COPD) and lung cancer patients by means of two haptoglobinspecific monoclonal antibodies. Haptoglobin N-glycans were then enzymatically released, fluorescentlylabeled and profiled by capillary electrophoresis. Disease associated changes of core and antennaryfucosylation were identified by targeted exoglycosidase digestions and their levels were compared inthe different patient groups. Terms such as of core- and arm-fucosylation degree, as well as branchingdegreewere introduced for easier characterization of the changes and statistical analysis was used toexamine which structures are responsible for the observed differences. Increased level of ?1-6fucosylated tri-antennary glycans was found in all disease groups compared to the control. Elevatedamounts of core- and arm fucosylation on tetra-antennary glycans were detected in the lung cancergroup compared to the COPD group. A larger scale study is necessary to confirm and validate thesepreliminary findings in the glycosylation changes of haptoglobin, so could then be used as biomarkers inthe diagnosis of malignant and inflammatory lung diseases.
Tárgyszavak:	Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban biomarker capillary electrophoresis
Megjelenés:	Electrophoresis. - 34 : 16 (2013), p. 2287-2294. -
További szerzők:	Mittermayr, Stefan (1983-) (bioinformatikus) Szekrényes Ákos (1983-) (vegyészmérnök) Kádas János (1976-) (molekuláris biológus, biokémikus, kertészmérnök) Takács László (1955-) Kurucz István Guttman András (1954-) (vegyészmérnök)
Pályázati támogatás:	K-81839 OTKA
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
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